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EMAGE:19444

Fbxo42 F-box protein 42 ( MGI:1924992)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19444 EMAGE:19444 EMAGE:19444 EMAGE:19444 EMAGE:19444
"Pseudo-wholemount" of euxassay_005149. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005149_01 euxassay_005149_02 euxassay_005149_03 euxassay_005149_04
EMAGE:19444 EMAGE:19444 EMAGE:19444 EMAGE:19444 EMAGE:19444
euxassay_005149_05 euxassay_005149_06 euxassay_005149_07 euxassay_005149_08 euxassay_005149_09
EMAGE:19444 EMAGE:19444 EMAGE:19444 EMAGE:19444 EMAGE:19444
euxassay_005149_10 euxassay_005149_11 euxassay_005149_12 euxassay_005149_13 euxassay_005149_14
EMAGE:19444 EMAGE:19444 EMAGE:19444 EMAGE:19444 EMAGE:19444
euxassay_005149_15 euxassay_005149_16 euxassay_005149_17 euxassay_005149_18 euxassay_005149_19
EMAGE:19444 EMAGE:19444 EMAGE:19444 EMAGE:19444 EMAGE:19444
euxassay_005149_20 euxassay_005149_21 euxassay_005149_22 euxassay_005149_23 euxassay_005149_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19444Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19444_wholemount_strong.wlz
19444_wholemount_moderate.wlz
19444_wholemount_weak.wlz
19444_wholemount_possible.wlz
19444_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19444_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
brain
moderate moderate
regionalmoderate expression: see section 09 10 weak expression: see section 02 03 04 05 06 07 08 11 12 13 14 15 16 17 18 19 20 21 22 23 24
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 08 20
trigeminal v ganglion
weak weak
regionalweak expression: see section 04 05 06 07 08 09 18 19 20 21 22 23
vestibulocochlear viii ganglion
weak weak
regionalweak expression: see section 06 07 08 09 18 19 20 21
spinal cord
moderate moderate
regionalmoderate expression: see section 09 10 weak expression: see section 11 12 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8820
Entity Detected:Fbxo42, F-box protein 42 ( MGI:1924992)
Sequence:sense strand is shown

>T8820
CGTCTCCCGGTCTGAGCAGCAGCGGCGGTGGCATCAGGGACGGCGGCGGCGGCGGTGGTAGCCGCGGCGG
CGTTTGAGTGGAGGAAGATGGCGGCTCCGGGCGACCCCGGGTTCCGCCAGCGGTGAAGATCCCCCGAGCA
CAGCAAGCACCGGCCTGAGGCAGAGCGGCTGTGCTCTGGCCCCGGCCCTGGCTCCTTCGCCACCGTCGCC
GGAGACGCGAAACCGAGAGCGCGGAGCGGTCTGGGCCAGAGCTTCGAGTGTGTCATGGCCAGCTCCTCGG
ACAGTGAAGATGACAGTGTCATGGCCGTGGACCAAGAAGAAACTGCACTGGAAGGGACAATGGAGCAAGA
TGAGGACCCCCACCCAGTGTTGGAGGTTGAGGAAACAAGACATAATAGGTCCATGTCTGAGCTGCCGGAA
GAGGTTTTGGAGTATATTCTGTCCTTCCTTTCACCATACCAGGAACACAAGACTGCAGCTCTGGTCTGCA
AACAGTGGTATCGACTTATCAAAGGTGTGGCCCACCAGTGTTACCATGGCTTCATGAAGGCTGTCCAGGA
AGGAAACATTCAGTGGGAGAGCCGGACTTACCCTTATCCGGGAACCCCTATCACTCAGCGCTTCTCCCAC
AGTGCGTGCTATTATGATGCCAATCAGTCTATGTATGTCTTTGGTGGCTGTACGCAGAGCAGTTGCAATG
CTGCCTTCAATGACCTCTGGAGGCTAGACCTAAATAGCAAGGAGTGGATTCGACCATTGGCCTCAGGGTC
CTATCCTTCCCCCAAAGCTGGGGCCACTCTGGTCGTGTACAAGGACTTGCTAGTGCTATTTGGTGGCTGG
ACAAGGCCAAGTCCCTATCCCTTACACCAGCCAGAGAGATTCTTTGATGAAATTCACACGTACTCGCCGT
CTAAAAACTGGTGGAACTGCATTGTCACCACGCATGGGCCTCCTCCCATGGCTGGCCATTCCTCCTGTGT
GATAGGTGATAAAATGATTGTCTTCGGTGGCTCCTTAGGATCCCGGCAGATGAGCAATGAAGTCTGGGTC
CTGGACCTGGAGCAGTGGGCGTGGTCCAAGCCGAACATCTCTGGCCCCAGTCCACACCCTCGAGGTGGCC
AGTCTCAGATTGTTATAGATGATACAACTCTTCTGATCCTTGGCGGGTGTGGCGGTCCCAATGCGCTGTT
CAAAGATGCCTGGCTGCTGCACATGCACCCAGGTCCCTGGGCCTGGCAGCCACTCAAGGTAGAAAATGAA
GACCATGGTGCCCCGGAGCTATGGTGTCATCCAGCCTGCCGGGTGGGACAGTGTGTGGTGGTCTTCAGCC
AGGCGCCAAGCGGGAGAGCGCCCCTCAGCCCCAGTTTGAACTCTCGCCCGTCACCTATCAGTGCCACTCC
TCCAGCCCTGGTTCCTGAGACCCGAGAGTACCGCTCCCAGTCCCCGGTGAGGAGTATGGACGAAGCGCCC
TGTGTTAATGGCCGCTGGGGAACACTGAGGCCCAGGGCTCAAAGGCAGACTCCCTCGGGTTCCCGGGAAG
GGAGCCTCTCCCCAGCCAGAGGAGATGGCTCTCCCATTCTCAATGGTGGAAATTTGTCTCCAGGGACAGT
AGCTGTCGGGGGTGCTTCCTTAGACAGCCCCGTACAGGTCGTGTCTCCAAGCACTCCATCTGCCTCTGAC
GGCTACGACCTAAAAGTAGGACTTTCTCTAGCTCCTCGACGAGGGTCTTTACCAGATCAGAAGGACCTGA
GGTTAAGTTCCATTGATCTGAATTGGGACCTGAAGTCTGCTTCCAGTAGTAGTCACGTGGATAGTATAGA
CAACAGGACAGTTGCAGGGAGTGTGAGACACCCTCCAGAACAGACCAATGGTGTTCACACCCCACCACAT
GTGGCCAGTGCCCTTGCAGGGGCTGTATCTCCCGGTGCCCTGCGTCGGAGTTTGGAAGCCATCAAAGCGA
TGTCATCCAAAGGCCCCTCAGCCTCGGCAGCACTAAGTCCTCCTCTTGGGTCTTCTCCAAGCTCCCCTGG
GAGTCAGAGCCTGAGCAGTGGAGAAACAGTGCCCAACTCCCGCCCAGGACCAGCCCAAGGAGATGGTCAT
TCCTTACCCCCGATTGCTCGGCGCCTGGGCCACCACCCTCCACAGTCCCTTAATGTGGGTAAACCCTTGT
ACCAGAGTATGAACTGCAAACCTATGCAGATGTATGTGCTAGACATTAAAGACACCAAGGAGAAGGGCCG
AGTCAAGTGGAAAGTGTTCACAAGCAGCTCGGTGGTCGGGCCTCCTGAGACCAGCCTGCACACGGTGGTG
CAGGGCCGAGGCGAGCTCATCGTATTTGGAGGGCTCATGGACAAGAAGCAGAATGTGAAGTACTACCCAA
AGACAAACGCCTTGTACTTTGTGCGCGCAAAGAGATAATGTGCTCAGCACCCCTTCCCTTCTGTGTCCTT
TAATTTGGAATTTTCCAGTGTGCAAACATTTGGACTGAGACTTGGGAAAACAATGCAACTCCCATAAACC
AAAACTCCAATTCCATGCCTAACTTGCTCCAGGCTGGGATTAAATCCCCACTAAGGAAAAGATTATATAT
ATACAGATATATTATATAGCACTCTGTTTACAATAAAGGCGAGATCTTCGTCCTGGTTCAGATCAGCTGT
TGTGGTCTTAGTGGAGGCTGTGCTGGCCTTTGTACTCTGCTGCTGACTAGACCGGGAAGTAAGGGGAGAG
ACCGCAGTAACTTCCCACAGGAAGCTGAAGAAGCGCCCCTGTGAGCTTTCTGTGGCCTTCTCAGAGTCAG
AAATGATGGTCTAGGCACTGAGGAAGAGGCTCTAGATGCCGCTGCAGGATCAGAGTCACAGAAGACAGCT
CTTCCCCGCCTCCTGTCAGCCACAGAGGACCCATGATGGCTCTGTAGAAGCGCCCTGTGCCTGGTCCCTT
CTGTCCTCTGTGATCCAGAGAAGTCACCCAACAGGAAACCCTGTTCCCCACGTGCTGGAGTCATGCTGAA
ACAATGTTTATAGAGCCGCCGTCCATCCGTCTGTCTGCCTCCTTCCTGTCCGTTGGTCTTTTTGAGTTCT
CCCTACATGTCTTAGCAAAACATTCAAATGTCTGATTAGGGAATTTTCCGACAATCGTGTTTGGTTGCAA
GTTGCCAAAAACCATAGTTGTTTTCCTTTTAGAACTGGGTCCTCAGAGGCCTCTCCTGTCTGCCGGCTGC
TGCCTGTCCTTCGGTGCTTTGCTTTGGGAGGTTTTCTTATACTTTTTGGTTTTTTTGGTTCATCTTTGTA
AAGCATCCGAACTTCCGAACTTCTTCCTCTCTGACAAAGCTCCAGTGTCCACAGAATTCAGAGCTGTTAG
AGCCTCCCGGCATCCTCATGGCCCGTCGGGACCCACCACAGTGTGCTGGTGCACGCCTGTGTCCTAGCAC
TCAGGGCAGACAGAGGCAGGATGACTTCAGGTTTGGGCTTAGCCTGAGTTATATGATGAGACTCTGTCTC
AACAAAACAGAGGACATCTGTAAAGCATCGAAAGACACCTTTTCCAGTTTTCTCTTTTTCTGAGGCAGTG
CCTCACTATGTACCTCACAGCGATCCTCCTGCCTCTGCCTGTCACTTGCGGCACAGCCCTCTGTGCTGTG
CAGTGCTGTGTCGTCTTTTTCTTGTGTCCCCCAAAGCAGAGCTTTCCTTGCTGCCTTCACTTCATTTCCT
AGAAGAGGTGCAGCCAACCAGGAGAGGGGTTGGTTGGTTGGTTGGTTGGTTTGTTTGTTCATAGAGAAAG
CTATAAATTATTGGTCTCTAAGCCACAGCCTAGACATAGGAGAAATCAAAAAAGGTTATTTCAAATGGCC
ATGCAACTATGGTTTGGTGGTTTCTCATCAGTGGATAAAGCATTTTTATTTTACCTTTAAAACTGAAATT
GAAAGGACAGCAGCGTGTTTCCCAGCGGCAGTCTCCCATTCCCCTGTCCCCGCATCTCCCAGGCACGAGC
CTGCTGCGGCTCGTCTGGCTCATGGCCAGCACTCACCTTCCGACAAGCGTGTCTCCTGCTCCATGCTCAG
TCTTTCCTTAGCCTTTAGCTTTGCTCTGCTGGTCTTGGCAAGAGAGCCCTCCTGTTTTGAAACTTGGAAA
AGGCCAACTCTAGAGGAGGGGTATTCGATCTGGAGCCGCTCCCGAGGGAGCACCCCTGTGCCTACTTCCT
GTGTGTCCCACTGCTCCAGCTGCCTGCTCTGTTGCTACTACCAGTCCTTTGGCCAAGACTGATCCTGTGG
GATCTTGACTCAAGAGATAAGGCCAGCAGATAAGCAGCCTAGCATTGCTGAACCCTCTCTGTGGTGTTTG
CGTGTGGATGGCGCCTCTGGATTTGGTCTCTACTGAAACATCAAGCATTCGGACACCTGAAAGTTAAGAA
TCTCTACTTTGTAGTCTTTCTCTTTCCCCTGTGGCCTTCCTAAGCTGGCTGTCAACTGTTGATTCTTCGA
CTTCTCCCAAGTAGACAGGCAACAGAAATCTTAACCTCTTAGAAAGCTATGGGTTCCTCTGCCTGAGATC
TCTGCAGTCCCTCCAGAGAAAACAGAATGAGCCTCGACTGCAACCAGCCATTTGGGACTTGATGGTCCCT
GAGTGCTGTAGATGTTGCTGTGGGAAGTGAATCAGCAGTGGAGACTGTGGTCTCCTCAGCCTCATGAGGC
TGTGTGCGTCCTACAACTTGCAGTACGCTAGCAGAGCTCAGTGTCCACAGAACACTGTCAGTTCAGATTT
AACCACCACCCCTTTTTTGACACGATACCAATTTTGTCTTAAAATTCACCAAGAGACATGAGACAAAATA
TTTTGTTTGAACCCTCAGGAGCACCTAAAGAAAATATTTATCAGTATTTAAGTATTTAAAATACATCTTG
TTTCTACTTGAAGCTTTAACCCTTTCCATTCCTGCAAGTGTGCCTTTGAGAGCCCTCGGTCATATTAACT
TCAGTCATTTGGTTGGCCTGTGAGAGTTCTGGGCGCTGCTCTGCTCCTGCACAGAGCTTCCTGGTCATGC
ATGCCACATAGTGTGCGGCAGAGAGCATTCGTTTCTCACTCCTGTTGCTGCCTTTCTTCCTTGTTGGGAC
GAGGGCTCCACAACCCCACCTGCCCCGTGTTTTGGGAGCACCAGTAAGGTAGAAGGAAACTGGAGTTTTG
TTGAAGGCAATGCTGGGCTTGCCCTGCCAGCCTTCTCATTTTAATGTCTGAGCTCTGGCGAGAACTGAAA
GCATGAACTGTGGAGGAACGGTCTCGGAACTCCCCTAACTTAAGAAGAGGAGAGAAGGCAGGATTGCAGG
AGGCTCAGCCTGGAAAGACAGACAGCCGCCGCAGTGGGACGAGGGGGCGCTCCCTTTACTTTGTCTTTAT
AAGTTCCTTCTGCCTCGCCTTTCCCTTCCTCTTCAGTCTGTCCATCTGTCTGTCCTTGTCCTCATGGTGA
CTCTGGCATGGTGACGTACTCTATTTGCATTATCTGTGGTCACCAGCAAAAATGACAGCGGGGAGGATCT
GAAGGAGTCCAGGGTAAGGAAGCCTGGCCTGGTGACTGCCTTAAGGCCTGCTCTGCTGTCATCTGCAGGT
GGCCTCTCAGCAGCCCCATCCTTGGGAATGAAAGCCAACCTCTGGCTCCACTGTGCTCGCTCTGCCCCCT
CCTACCAACCAGGGCTCACGTCGGTGCACGCCCACCGTGCCCTGACAAAGCTGGTGCTGTGAGTGATGTT
TCCCATACAACTCAGGGGTGCCAGGCGGCTCCCCCCATAGTCATTTTAGGCACATCACAGTGTGGGAATG
AAAATGGATTCCATGGATATTTAAATCTGTCCATTTCATTTTTGTTAATGTTTCCCCCGATGACTTTTTA
GCAGTTTAACAAATAAAACAACGGACTGAATTGTCAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6410012 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6410012 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19441 same embryo
 EMAGE:19442 same embryo
 EMAGE:19440 same embryo
 EMAGE:19443 same embryo
 EMAGE:19445 same embryo
 EurExpress:euxassay_005149 same experiment
 MGI:4824809 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS