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EMAGE:19446

Rpl31 ribosomal protein L31 ( MGI:2149632)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_005196_01 euxassay_005196_02 euxassay_005196_03 euxassay_005196_04 euxassay_005196_05
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euxassay_005196_06 euxassay_005196_07 euxassay_005196_08 euxassay_005196_09 euxassay_005196_10
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euxassay_005196_11 euxassay_005196_12 euxassay_005196_13 euxassay_005196_14 euxassay_005196_15
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euxassay_005196_21 euxassay_005196_22 euxassay_005196_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19446Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19446_wholemount_strong.wlz
19446_wholemount_moderate.wlz
19446_wholemount_weak.wlz
19446_wholemount_possible.wlz
19446_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19446_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8834
Entity Detected:Rpl31, ribosomal protein L31 ( MGI:2149632)
Sequence:sense strand is shown

>T8834
CGCGCCCTTCCTTTCCTACTTGGGCCCGGCAGAATGGCTCCCGCAAAGAAGGGTGGCGAGAAGAAGAAGG
GCCGTTCTGCCATCAACGAGGTGGTGACCCGAGAATACACCATCAACATTCACAAGCGCATCCATGGAGT
GGGCTTCAAGAAGCGTGCTCCTCGGGCACTCAAAGAAATTCGGAAGTTTGCCATGAAGGAAATGGGGACA
CCAGATGTGCGCATTGACACCAGGCTCAATAAAGCCGTCTGGGCCAAGGGAATAAGGAACGTTCCATATC
GCATCCGAGTACGCTTGTCCAGAAAACGTAATGAGGATGAGGATTCCCCAAACAAGCTCTACACGCTGGT
AACTTACGTGCCTGTTACCACATTCAAAAATCTACAGACGGTCAACGTGGATGAGAACTAACCTGCTCGT
GTCAAATAAAGTTGCAGAACTGCCTTCAGGGTTTGGTTTTCCTTTCTAAAAAAAAAAAAAAAAAAAAAAA
AA
Notes:The probe template was PCR amplified from IMAGE:6410850 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6410850 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19448 same embryo
 EMAGE:19451 same embryo
 EMAGE:19449 same embryo
 EMAGE:19447 same embryo
 EMAGE:19450 same embryo
 EurExpress:euxassay_005196 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS