EMAGE mouse gene expression spatial database

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EMAGE:19451

Ankle2 ankyrin repeat and LEM domain containing 2 ( MGI:1261856)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005145. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005145_01	euxassay_005145_02	euxassay_005145_03	euxassay_005145_04

euxassay_005145_05	euxassay_005145_06	euxassay_005145_07	euxassay_005145_08	euxassay_005145_09

euxassay_005145_10	euxassay_005145_11	euxassay_005145_12	euxassay_005145_13	euxassay_005145_14

euxassay_005145_15	euxassay_005145_16	euxassay_005145_17	euxassay_005145_18	euxassay_005145_19

euxassay_005145_20	euxassay_005145_21	euxassay_005145_22	euxassay_005145_23	euxassay_005145_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19451_wholemount_strong.wlz

19451_wholemount_moderate.wlz

19451_wholemount_weak.wlz

19451_wholemount_possible.wlz

19451_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19451_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8823
Entity Detected:	Ankle2, ankyrin repeat and LEM domain containing 2 ( MGI:1261856)
Sequence:	sense strand is shown >T8823 CCTGATCGTTGGGAAACGGGACGGCCCGCACTGCGCGCGCAGCCCGCCTGGTCTAACCACGGCCGGGCGG AGGAGGAGGCTTGGGGCAGGCGCGACTCCCGTGGCGAGGCGAGCGGTCTTCTGAGGGCCGGAGGCGGGAG CGACGCGGGCTGCATTTGCAGCGGCCGCGCTCAAGGCCTCGGCGCCGGCGGTGGCGATGCTGTGGCAGCG GCTGGCTGTGGTGGAGTGGGCGGCCCTGGCCTGGGAGCTCCTGGGCGCCTCGGTGCTGTTCATCGCCGTG CGCTGGCTGGTCCGCCGGCTAGAGAAGCGGCCGCGGGACCTGAACCGTTGCGGGACGCTCTCCTCTCCGC CCAGCGCGTCTGAAGCGGTGGCCGCACAGCCAGGTGAAGTGACAATGGATGCCATGATGGCTCGACTGAA ACTCTTGAATCCAGATGACCTTAGAAAAGAAGTTATGAAAGCTGGACTTAAATGTGGACCCATTACATCA ACAACAAGATTTATTTTTGAGAAGAAATTAGCTCAGGCCTTACTGGAACAAGGTGGATTGCTGACCTCTT CCCTTCCCAAGCCCAGCGCGGTTACTGCCATGGCATTTATCCAGGGCACCTCAAGGACTCCTCCATCTGT GGACGGGAAACAAACTCAGCAGGCCTGTTTTTCTGAAGACAGAGATTTTGGTTACAGTGTGGGCTTGAAC CCTCCAGAAGAGGAAGCGGTAGCATCCAGTGTCCACCCAGTACCCTTCAGTGCCTCTACCAGGAATGACA ACCACAAGGCTGGAGTGACAGCTCCTAAGGAGCCACTTGTGTACTATGGAGTGTGTCCAGTATATGAGGA TGGCCCTGTGAGACATGAGAGGATCCATGTTTACGAAGATAAAAAGGAAGCTTTACAAGCTGCCAAGTTG ATCAAAGGGTCCCGGTTTAAAGCTTTTCGAACAAGAGAAGATGCTGAGAAATTTGCTAGAGGGATATGTG ATTATTTACCTTCTCCCAACAAAACTACGCCCTTACTGTCTCCTGTGAAGGCAGTGCCCCTCGGTGGCAG TGATGGGCTGAAAGATGGCTTGTGCCTGGCTGAATCAGAAACAGTAAACAAAGAACGAGCAAACAGTTAC AAAAACCCCCGTACCCAGGACCTCACGGCCAAGCTGCGGAAAGCTGTCGAAAACGGGGAGGAACACACCT TTTCTGATCTTATCTGGAGTAATCCTCGATATCTAATTGGTTCTGGGGACAACCCAACCATTGTACAAGA AGGATGTAGGTACAATGTCATGCATGTTGCTGCCAAAGAGAACCAGGCTTCCATGTGCCAGCTGACATTA GAGACTCTGGAGAACCCTGAGTTTATGCGTCTGATGTACCCAGATGACAACATGGACATGCTACAGAAGC GCATCCTCTACGTTGTTGACCTGTATCTTAATACTCCTGACAAAGTGGGCTTTGATACACCACTGCATTT TGCTTGTAAATTTGGAAATGTGGATGTAGTCAATGTGCTTTCTTCACACCCTTTGATTGTGAAAAACCGA AAGAATAAATATGGTAAAACACCTGAAGAAGTAATTTGTGAAAGAAGCCAAAACAAATCTCCAGCACTGA AAGAAAGGATTAGAGAATACTTAATGGGTCACTACTATGTGCCACTCCTGAGAGCAGAAGACACTTCTCC TGTCATTGGGGAGCTGTGGTCCTCAGACCAGAAAGCTGAAGCCTCAAATACTGCCCACTGTAGAAGCAGC CCCAGAGATCCTGTGATGACACTGAGAGCTTTCGTGGGACCTCTGAGTCCATCCAAAGCAGAAGATTTTC GAAAGCTCTGGAAAACTCCACCTCGAAAGAAAGCAGGCTTCTTCCACAGCATCAGAAAGTCTGACCCAGA ACGAGGCATTGAGAGAGTTGGAAGGGAGCTAGCTCATGAGCTGGGGTATCCCTGGGTTGAATACTGGGAA TTTCTGGGATGTTTTGTTGATCTGTCTTCCCAGGAAGGCTTGCAAAGACTAGAAGAATACCTTATCCAGA AGGAGTTAAGCAAAAAGGCTCAACAAGAAATACGAGAAAATGAAGGCTGCCTTCAGGACAGAACCTCAGA TTTTGGCAGTGGAAAGAAGTACAGCAACTCCATCTCCGTGGGAGCATTTCTAGATGGGGATGATGACAGT AGTTTGGAAGAAATTAAAAACCAGCAAAATACAGTTCCAAGTCAGAGTCAGCCCACAACTGATAAGTTCC AAACCTCTAAAAGTGGTTCCCTTCCCTTGGGACAGAAGGTCGACCCTGGAGAAACCTCAGTTGGAACTTA CCCAGATAAAGGCAGAAATGGGTTTTGTCACCCTCTAAACCACAGGACTGCAGATGGAAGGGGACTAGAG GCCACCAATGGGGAGGAGGCCCTTCCACCACCTGTTTCTGTTTTGACTCAAGAGTTGAATAAACTGAATT TGCAAAGCCTGGGAGACAGCCTTCATGAGACACCAGATAAAAACGGGAAACTAGAGGATGAGGTGCTTCC TTCAAGAAAGGGTGCAGCAGACAGTGACTTGCTGGCATCTCCTCCTGCCATAGCAAGCCTTGGAAAGAAG CAAGTCAGGACCAACACTGAGGTGTCAGAGGCAATGGCTGAAATGTCTTTGGGCCCTAAGAGCCCCCAAC TTGGAGTTCAGGCTGGTCTGGAACCTATACTCTCATCTGCCACGGTAGATTCCACCAAGAGGCTCTTCCT GTCTGGAGAGGAACCATCAAAACTAGATCGAGATGTTTTAGCAGCTCTTGAGTGTGCGAATATTGACCCT GGTCTGTACCCAGCAATCCACAGATGGAAAAGCACTGTCATGTGCTACTCGCCCTCAGACAGACAGAGTT GGCCAAGTCCTGCACTCAAAGGGAAGTTCACAACTGAGTTGGTGGATCTCGATTGCTCTCATAGCTGCTC AGGACGATGCAGTCCAGCTGGTAGCAGCCCCAGTAAGCCTGGCCACACCTCCTCCTCTTCTGGCCTGCAC AGCCCTGGACGCTACAGTCCAGCACATGGGAGACACTTCCAAAGAGTGGCACACGTGGCCCGACTTGCAG CTCTGTAGAGCCAGCTTACTCCTGGGATTCATTTACTTCTTTGTTTGTAAAAGAGTAATGTTTATTGGTA AAAATTCCATGAAAGAATGTATCCTGAATAACTTAATCCTCGCTTTGAGGGTACTGGTATTTTAGAAAAT ACAATGTTCTGAGTATAATTTGAATGTCTGATATTTTGGAAGAAGTTAATGAAAGCAAACTCAGATACTA ATTGTTTGAAAGTGTAGCCGTAATAATGATTCAGACTTTTCAGGTGGAAACTTCTAATTTTGAAATAGAA CAAAGATGCAAAGGCATAGACAAGTGAGGTGTTGCTAATTGGGTTTGTTAGCAGCAACACTGCAGCACGT GCACACACCCACTATTTCTGAAACAGATCAATTATGTATTGTCTCTTGGGGGGGTGCAGCTTTTTGATAT GCAATGGCAAAACTATGTGTTTGTCATTTCACCCTAGTTATTCCAACCGAGAGAAAGCAGGTGGCAGAAA AGCAGATGTGCCCTGACTCAGACTTCAGTAACAATGCTCAAAACACTGCCAGAAGTTGGGATGGGGGGTT AAAGCCATCGAGAATCATGCGAGTAATCATCATAGAAAACTCACCAGCAATAACTAAAACCAAAACTATT TCTCAGTACTTGTCAGTGTAGTAAGTTGTCTAGAAGTTGTACCCAGGCTGGAGAAATGGCTCAGCGGTTA AGAGCACCGACTGCTCTTCCAATGGTCCTGAGTTCAATTCCCAGCAACCACATGGTGGCTCACAACCATC CATAATAAAATCTGATGCCTTCTTTTGGGGTGTCTGAAAACAGATACAGTGTACTTACATATAATAAATA CATCTTTTATAAAAAAAAGAAATTGTTCCCAAGTCTTAAAATTTAGCCTGAGATTTCACTAAGCATGATT ACATGACAGATGTATGCCGTATTTTAGAAATTAAAGAGCTTTATGCTAGAGTTTAGCACTTGAGATCTGA GTTCATTTCAGTGGCTTGCAGTGTTGACTTTTGGCTATCCCAAGAGATGTGTACCGAGTGGCATTGCACA CCCTAAGGCTCCAAGATAAAACCACCTTGCTGAAGCTGGGTCTCAGCTGTCTGTCAGTTGCTTGCACAGA GCCAGTAGTGTTCTGGAGACACTTGCAGTGCTTCACCCCAGTACAGAACTATACCACTGATTTTGCCAAT TTCAGTGCCTTTTGTGAGTCATTTTGAGATCACATAGTGCTAAATGGGGGCGTTAGATTCTCTTTTGTTT ATAAAAATGTTGACCAGGCAAGCCTTTAAGCCCAGCACTCATTAGGCAGAAGCAGGGGGTTCTCTGAATT TGAGGCCAGCCTGGTCTAGATAGTGAGTTCCAAGACAGCCAGAACTACACAGAGGATCTTGTCTCAAATT TAAAACAAAAACAAAAACCCACCAAAATGATAATAAAGTAATTCTGAGCTCAAATGTTTAGGTCTTTTCT TTTTTCTTTTTTTTTTTTTTTAACTTTTTTAAAGCTTATTTTAATGTGGTAAGAAATACCAGAATTGAGA TTTTGGACTTTGGATTTAGTTTGTTTTGTTCCCCTTGTAAGCAGCTGAGCTCTAAGTACAAGACAGCCAA TCAAGTGACCTGGAAACAATCCCAAAGGGAATCTTTGTTCTCAAAAGAAACCAAAACCCACACACTATAT TCAGCTATGATTTTGTTTTCTCTAAAGTCTTATTGTCCTGTGCGGTTATTTTTGTTGTTTTTTCCATACT CAAAAACTGAAGTACTAGTTTTGCCCTATTTTAACTTTTTTTTTTTTAAACTCTTCTTTCTTGATATTTA CTTAAGACAACTTTTGGGAGTCAGTTCTCAAACCATGTAGGTCTCAGGAATCCAACTCCAGTCACTGGAA TTGAGTTTTACCCGAGCCATCTTGCTCGCCCATTTTAAACCCTAAGCTGCCTTTGGACTATTTTTCCACT AGTATGAAGGGTATATACCTGTTAGTAACACCTGTTGATCTATGTCATAAACATTGTTTACTTGATCTTT CGCAAAGTGTTGGGTAGGAAACGACTCCTTAATTTGTTATTGGACAGAATCCCCTTCTTAGTAAAACTGT AAGAAATATTTTCATTAAAAAGGACATTGAGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6410033 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6410033 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19448 same embryo
	EMAGE:19449 same embryo
	EMAGE:19447 same embryo
	EMAGE:19450 same embryo
	EMAGE:19446 same embryo
	EurExpress:euxassay_005145 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source