EMAGE mouse gene expression spatial database

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EMAGE:19456

Impa1 inositol (myo)-1(or 4)-monophosphatase 1 ( MGI:1933158)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005188_01	euxassay_005188_02	euxassay_005188_03	euxassay_005188_04	euxassay_005188_05

euxassay_005188_06	euxassay_005188_07	euxassay_005188_08	euxassay_005188_09	euxassay_005188_10

euxassay_005188_11	euxassay_005188_12	euxassay_005188_13	euxassay_005188_14	euxassay_005188_15

euxassay_005188_16	euxassay_005188_17	euxassay_005188_18	euxassay_005188_19	euxassay_005188_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19456_wholemount_strong.wlz

19456_wholemount_moderate.wlz

19456_wholemount_weak.wlz

19456_wholemount_possible.wlz

19456_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19456_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8857
Entity Detected:	Impa1, inositol (myo)-1(or 4)-monophosphatase 1 ( MGI:1933158)
Sequence:	sense strand is shown >T8857 TGCGCTCGCGCGAGATATGGCAGACCCTTGGCAGGAGTGCATGGACTATGCAGTAATCCTCGCGAGGCAA GCTGGAGAGATGATTCGTGAAGCTTTAAAAAATGAGATGGATGTCATGATTAAAAGTTCTCCAGCCGACT TGGTAACAGTTACTGACCAAAAAGTTGAAAAAATGCTCATGTCTTCTATAAAGGAAAAGTATCCATGTCA CAGCTTCATTGGTGAAGAGTCTGTGGCAGCTGGGGAGAAGACGGTCTTCACAGAGCAGCCCACGTGGGTC ATTGACCCCATTGATGGAACGACTAACTTCGTGCATCGGTTTCCCTTTGTAGCTGTTTCAATTGGCTTCC TTGTGAATAAAGAGATGGAGTTTGGAATTGTGTACAGCTGTGTGGAAGATAAGATGTACACCGGCAGGAA AGGGAAAGGTGCCTTTTGTAACGGTCAGAAGCTTCAGGTGTCCCAGCAGGAAGACATTACCAAGTCACTC TTGGTGACCGAGTTGGGCTCGTCCAGAAAGCCCGAGACTTTACGGATCGTTCTCTCCAACATGGAAAAGC TGTGTTCCATCCCCATCCATGGAATCCGGAGTGTTGGAACAGCTGCTGTTAATATGTGCCTTGTGGCAAC GGGAGGAGCAGATGCCTATTATGAGATGGGAATCCACTGCTGGGACATGGCGGGAGCTGGCATCATTGTC ACCGAGGCAGGCGGAGTGCTCATGGATGTCACGGGTGGACCGTTCGATCTGATGTCTCGGAGAATAATTG CCGCAAATAGTATAACATTAGCCAAAAGAATAGCCAAAGAAATTGAGATAATACCTTTGCAAAGAGACGA CGAAAGCTAGTCACAGAGAACAGTGTCCAGCTCCAGTGTCATCCTTGCTGTCCCTGGGGTGTTTCAGATG GATGGTGTCACTGATTTAGACTGAACTTTGAGGTCCTGATTTTAAAATGGAAACTTTTTTTTTACAGATG ACATATTCAAAATTAGATGGAATATTTGATTATTGAAAGAAAATTTGCATGTAGTAATATTCTTGGGGAA AATATACAAAAAGTATACTTAATGAACTAGCCATTGAAATTGTCCCTAGTCCTTATGATCCCCTTCAACT TAATGTACTGTTTATATGCATAATTCTCAATTACAAAGTTTCTTTTTGTAAGTGGCTTTCTCTATGTTCC AGAAGCCATATTTGATTAAGTCTAAAGGCTGTAACAAGCTGGCTCTCCCTGTGCAGAGGGCCTTTGTGTT TTATTAATCACTGTAAGATAGTGCCTGGCCCAGTGCCTGTCAGACAGTAGGCAGTCTGAAGTCCACACCT GACAATGCGTGCTCGAAGCTGCAGCTGCTGCCTCTAATGCGTCACAGTAAGATAACCACCCTCCTGTTGC GAGGTAGAAGTTACTTCACTGTCCTTTTTATATTTCTTATTGCTATGCCATTTCACAGGATCGTGCTGCC AGAGACGACTGCTTCTAGTGGACATTTCTGCAGTTAGTACACTGCTGTATGTTGTAGGTTCTGCTTAAAG CTGCCGTGCTAAAGAGATTTTCACAGACATCTTCCAGGTACCTGGTCTAGTTAGTGGCAGGGATATGTTT TACAAAAGGCAGCTTTCTCATTCAGATCCGTACCCTGGTGCTGACCTGTGTACTGTGGTGTAATGGTGAA CTTTTTGATTTCTTTCCAGACTTGCTGAATTTCATCACTGCTAACTCTAGATGCTCTCTCTATAAGGTCT TGGGCCTCTCAAACTCAAGAAAATTTAATGGCTCCTATTCCTTTGTTAAAGGGTTAATTCATTGTCTAGC CTTGGCCCTTGGCATATGAACAGATGTTTTGCTCTTAGTATGTTTGAACCTTGCATTTGATACAATGAAG TGTTTTTGTAAGTTTCAAGGCAGTTATCTTGATTTTGGGGGGATTTAATATATTAAAGCTATATAATACT CAGATTTGGGCACTGTAATGACTATATCTGTGCTGTTAATTACATGTATTTAAAACGTCACGTACCATGT AAATTCTATTACAAGACAGGTTGCTTTGCAATTAAATTTATTTTAGTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6413389 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6413389 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19454 same embryo
	EMAGE:19457 same embryo
	EMAGE:19455 same embryo
	EMAGE:19453 same embryo
	EMAGE:19452 same embryo
	EurExpress:euxassay_005188 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source