EMAGE mouse gene expression spatial database

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EMAGE:19457

BC052040 cDNA sequence BC052040 ( MGI:3026886)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005181. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005181_01	euxassay_005181_02	euxassay_005181_03	euxassay_005181_04

euxassay_005181_05	euxassay_005181_06	euxassay_005181_07	euxassay_005181_08	euxassay_005181_09

euxassay_005181_10	euxassay_005181_11	euxassay_005181_12	euxassay_005181_13	euxassay_005181_14

euxassay_005181_15	euxassay_005181_16	euxassay_005181_17	euxassay_005181_18	euxassay_005181_19

euxassay_005181_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19457_wholemount_strong.wlz

19457_wholemount_moderate.wlz

19457_wholemount_weak.wlz

19457_wholemount_possible.wlz

19457_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19457_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 07 08 10 11

submandibular gland primordium

moderate

regional

moderate expression: see section 06 13 14 15 16

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

adenohypophysis

weak

regional

weak expression: see section 08 09 10 11

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12

spinal cord

moderate

regional

moderate expression: see section 05 06 07 08 09 10 not examined expression: see section 04

retina

moderate

regional

moderate expression: see section 01 02 03 04

neural retina

moderate

regional

moderate expression: see section 20

metanephros

moderate

regional

moderate expression: see section 03 04 05 06 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8620
Entity Detected:	BC052040, cDNA sequence BC052040 ( MGI:3026886)
Sequence:	sense strand is shown >T8620 CGCCCCGCGGTGCAGCTAGGGGGGTGTGCTTTGGGGGCATAAGGGATAGCTAAATAAGTGGAGGGCCCAG GGTCGTACCGCGCTCTGCCCTTCCCTAGGTTCTAGGCACCAAGCCCGCACGAGCCCAAGGGTGATTTTTC CTTGGGCCCAATATGATACTAACCAAAGCCCAGTACGAGGAGATAGCCCAGTGCCTAGTGTCTGTACCGC CCACTAGGCAGAGCCTGAGGAAGCTGAAGCAGAGGTTCCCCAGTCAATCGCAGGCCACTCTGCTGAGCAT CTTCTCCCAGGAGTACCAGAAACATATTAAAAGAACACATGCCAAACATCACACTCCAGAAGCGATTGAA AGTTACTACCAGAGGTACCTGAATGGCGTGGGGAAAAACGGAGCAGCCCCCGTGCTCTTGGAGCTGGCCA ATGAGGTGGACTATGCACCCTCATTAATGGCTCGGATTATCCTGGAGAGATTTCTACAGGGGCATGAACA GACTCCACCCTCCAAGTCTGTTATAAACAGTATGCTGCGGGACCCTTCTCAGATTCCAGATGGAGTTCTA GCAAACCAGGTCTATCAGTGCATCGTGAATGACTGTTGCTATGGACCGCTGGTGGACTGCATCAAGCACG CTATTGGCTATGAGCATGAAGTCCTGCTGAGAGACTTGCTTCTGAAGAAAAACCTGTCGTTCCTAGATGA GGATCAGCTCCGTGCAAAGGGCTATGACAAAACCCCAGACTTCATTTTACAGGTGCCAGTTGCTGTGGAA GGGCACATAATTCACTGGATTGAAAGCAAAGCCTCATTTGGTGATGAATGTAGCCACCACGCCTACCTGC ATGGCCAATTCTGGAGCTACTGGAATAGGTTTGGACCAGGCCTGGTCATCTATTGGTATGGATTTATCCA GGAACTGGACTGCAACCGGGAAAGAGGCATCCTGCTCAAAGCCTCGTTCCCCACAGACATCGTCACCTTA TGCCATAGCACAGCTTGACCCCGAAGATCCTGGAAGAGAAGCCGGAAGCAATGTTACTTTCCTGCAGTAT AAACTGCTGGCAGCATCTGCCCTGAACTTGAGATGAACCCTTGCTGCCGTCGCAGCCCTACCTCTAGACA AACATATCAGGAGTTTCTGTTTTCCTTCATCCCTGGGTGATGTGAATAGCCAAGAACTGCAGACACAACC CACTCATTATCAGCATTTCTGTCTCTGACAAACAGTTAGTTTATAGATAGTCATAATCTTGCTTCCTTCC GAATGCTTTCTCCTTGTATACTGAACAGAAGAGAAAGTATGGAGACTGCAAGATGTGAGTTTTCACTTCT CCTCCCTGTGATCAAATATTTTTTCTTCTAACCCTCCATTCATATTCTCTCTCTCTCTCTCTCTCTCCCT CTCTCTCTCTCTCTCTCTCTCATATTCTCTCTCCCCCTCCCCACCCCACCCCCGGTCTCTTGGCGGGTAC ACTGGTACTGAGTGTTGCATTACCGGGGAATAACCTAGCACAATCCTAGAAAAGCTGGAGACAGGAAGTC CGTAAGTAAATGTTCCTTCTCACCTGGGGCTCAGCAGAGGCCGCCCGGAGTCTTTCTGTGGAGCCTGGTG GCCTCCTGACTTCTGTGGCATTCAGCCAAGCTGCACAAGTCCCCCTCGATGAGAATTTCCTCTTTTCATA AAGTTGATGTGAACACCAAACCCTTCACAAGGCTGACCTCCTCTTCAAGTATGAGTTGCCGCTGTGTCTG GAAAACCATGCTCCTCTAGCTGGGGGCTGCAGCAGAGGACTGCACCCCGGCCTCTGGTCTGCAGTGGCCC TCTAAAGCAGACTGGCGGCCGGCAGGAACAACCTTAGCAGCTATCATTATGAGATTTTTTTTTAAAGTAC ATTCATACATACTGCTTTGAGTCTGCATTTTATACCACATAATTGTTTAATACTCAAATGATTCAGACAT TATAAATAGTCAGCTTTGAATGAATTAAGTGTACCAGAAAAGTTCTTGAGTGATTTTTAAATAAGCAATA TAAAAGAGATTTTGCATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5718089 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5718089 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19454 same embryo
	EMAGE:19455 same embryo
	EMAGE:19453 same embryo
	EMAGE:19452 same embryo
	EMAGE:19456 same embryo
	EurExpress:euxassay_005181 same experiment
	MGI:4823430 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source