EMAGE mouse gene expression spatial database

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EMAGE:19482

1700030K09Rik RIKEN cDNA 1700030K09 gene ( MGI:1919504)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004874. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004874_01	euxassay_004874_02	euxassay_004874_03	euxassay_004874_04

euxassay_004874_05	euxassay_004874_06	euxassay_004874_07	euxassay_004874_08	euxassay_004874_09

euxassay_004874_10	euxassay_004874_11	euxassay_004874_12	euxassay_004874_13	euxassay_004874_14

euxassay_004874_15	euxassay_004874_16	euxassay_004874_17	euxassay_004874_18	euxassay_004874_19

euxassay_004874_20	euxassay_004874_21	euxassay_004874_22	euxassay_004874_23	euxassay_004874_24

euxassay_004874_25	euxassay_004874_26

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19482_wholemount_strong.wlz

19482_wholemount_moderate.wlz

19482_wholemount_weak.wlz

19482_wholemount_possible.wlz

19482_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19482_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6553
Entity Detected:	1700030K09Rik, RIKEN cDNA 1700030K09 gene ( MGI:1919504)
Sequence:	sense strand is shown >T6553 CGCACACTGGGGCCTATACTTTGAGGCTGAAGCCTAGCGGGAAGAAGCCAACGCAGCTACTCAGAATGGC TTCCACAAGAAGACCCTTTGGCCCTCCACGTAGTGGTTTTGACTTAAGGGATATGTTCTTAGATAGCTCG AGGATGGAAGAGATCAGAAACTTACAAGCCCGAAGTCTTGGCCAAGTGACACCTGGGCAGAGCAGAATCT TAAAGAGAAACCAAACGATGGACGAGAAATATTTGATGCCCAAAGAGGAGGCCATGGCAGGGAGGGGGGT GATTCTGGGCTTGAGGCCTCCCACCATCTCCTCCAAGACCAGAGCCAGTGAGGCCCTTAGGAAACTGGCC CAGATTGAAACCAAGATCCTGAATCGGAAGCAGGTGCCCATGGCTTGGTCTGATGTGGAGTCTGACTCCA CATCCATCGAGCAGAGTCTTCCTAAGAGGACAGGTGCAGCTTCTGTTTCTTCCCAGTACCCACACAGAAC TTTCCAGAAACAAGTGAACAAAACATGTGTGTCAAAGAGCGATGGGCCGAGTGGTAACGGTAGCAGGTTT CTGAAGAAGAAGGAGCTGCCCACTGAAGCGAGGTCCCCAGGGCTGGCAGTGGGGACAGGGAAGCAAGCTC TGCTGCCCACAAAGAAAGAATCTGCCAGTGACGAGGAGGAGGAAATGCTACTGCTTGGAAGCTTGATGGA ATCTTCTAGAGAAAAAGAAGCAAATAGAAACCAGGAGCTCCCTGGCTCCAGTGTCAGCAGGAGCAACCTT GGAAAGGTGTTCTTGGATCCAACTCCAGATCAGCCAGGAGTCCTGTCTCTACTCAGTGTTGACCAGTCCA GCTTAAAGTCTCCTCGGCCCATACAGTCGACTCGCGTTGGCTTGAGAACTCACTCCCGACAGGCGAGCAG TGCTGGGGACACAGTCTCCATGACAGCCTCACCTCCCATCCTTGATGACTTCTCCAAATCAGCATCTTCA AAGATGGGATGCATCAAGCTGGCTTCCTCCCCCAGCAGGACGGAGTTGGAGTCTTCTGAGGAACCAGTCT CAGAAGCTGCTGCTGACAGCCTCCATGATTTTAGAATTAATATTTTGTCGATTGATGATCTGGTTCTGGC TGACGGAGACAAATCAGATGGGGACCAGAGAGAGGAAGACTGTGTAAGGGAAGGGATTTCAGTCAGAAGC TCCTCACCCACATCACCTACTCGACTGGAAGCGCAGATGTGGCCCAAGACTTGTGTGTTTCAGGGCGCAG CCACCGTGGTTGGGGATGAAGAGGACCTTACCACTGAGAGTGACGTCTCAGAGCCCCCGGGGACCAGCTC CTCAGCAGCTGTCCAGTCTCACAGTATGTCCAGGGCCCTCACTGCAAGCCCAGCTTACTCAGAGGATTTT GAGCAGTTCTCTGGTCCCTTGGCCTTGGAGGAGTCGCTTGACAGGACACTGGACACTTTGTCAAAGTTTT CTTCCAGTGGTCAGACAGATATCGTTGCCAGACAACCTCTATCAAGGACAGAATGGGGCAGGGGTGTGAC CAGAGTTGTGAAGGAGACAGCTGTGCAGACACTTGATCCTGCCTTTGCCTACCAGTGGAGCAAGGCTGGT GGCATAGCAGCTGTTGGACCTGCCCTCGGAGGTGCTTATGTGGACCCAGCACCCATCGCCAGTCACATTG TCAGCGCAGACGCCATAGAAGCCCTGACAGCCTACAGCCCGGCAGTTCTGGCGTTGAATGACATGCTAAA GCAACAACTGAGCCTGACGCAGCAGTTCATCGAGGCCAGCCAGCAACTACATGGTTCTCTCCTGCAGTCC CTGGATGGGGACTCATTTCACTACCATACCTTGGAGGAAGCAAAAGAGTACATCAGGTGCCACAGGCCTG CACCCCTGACCATGGAGGCTGCCCTGCAGGAGGTGAGGGAGGAGCTCCAAGTCCCAGCAAGTGAGGCTTG CCTGGGTACCTGTCCACCTCGGAATCAATGACATCCATATGGAGGCAGGCAAGGTTGGGGTTCTCCCTGC TCAATAACAACCATATATGGACTCTACGCTCCCATCTGCCCAGCTGGCTGCTCTGAGGATACGGCATGAG AACAGCTGAGAACCCACCACAGGACAGTGGTGTGGTATGGGGCCATGAGCCAGTTGGACAGACAACATGT GTCAGGCATTCCAATCATCCAAGATGGGACCAGCTGCCCCATGGGAACACTCCATGCCAGGTGCTGAAAC AGGTATGAGCCTGGTGCCTGGGCACAGCCTGCATCTATCCAGAGCAGCCTGCACAGTATCTCTCATCCAC AGCTAGAGGAAATCTTTTACAAAAGGCTTTATTGGAAGGCAAAACAGTAAAATCCACAATTGTTAACAGC AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3592293 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3592293 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19481 same embryo
	EMAGE:19484 same embryo
	EMAGE:19483 same embryo
	EMAGE:19485 same embryo
	EMAGE:19486 same embryo
	EurExpress:euxassay_004874 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source