EMAGE mouse gene expression spatial database

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EMAGE:19486

Fam132a family with sequence similarity 132, member A ( MGI:1914639)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005009. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005009_01	euxassay_005009_02	euxassay_005009_03	euxassay_005009_04

euxassay_005009_05	euxassay_005009_06	euxassay_005009_07	euxassay_005009_08	euxassay_005009_09

euxassay_005009_10	euxassay_005009_11	euxassay_005009_12	euxassay_005009_13	euxassay_005009_14

euxassay_005009_15	euxassay_005009_16	euxassay_005009_17	euxassay_005009_18	euxassay_005009_19

euxassay_005009_20	euxassay_005009_21	euxassay_005009_22	euxassay_005009_23	euxassay_005009_24

euxassay_005009_25	euxassay_005009_26

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19486_wholemount_strong.wlz

19486_wholemount_moderate.wlz

19486_wholemount_weak.wlz

19486_wholemount_possible.wlz

19486_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19486_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thyroid gland

moderate

regional

moderate expression: see section 09 10 14 15

vibrissa

strong

regional

strong expression: see section 05 06 07 08 09 21 22 23 24

cochlea

strong

regional

strong expression: see section 01

cochlear duct

strong

regional

strong expression: see section 02 03 04 05 06 16 17 18 19 20 21 22 23 24

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23 24 25

kidney calyx

moderate

regional

moderate expression: see section 05 06 07 08 09 10 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6878
Entity Detected:	Fam132a, family with sequence similarity 132, member A ( MGI:1914639)
Sequence:	sense strand is shown >T6878 CGGACGCGTGGGGAAAGTAGTTCTCTTTCGGTGGCAGGGTTGCTGTCGAGGGCACCGAGCAGGAGGATAG GTCGACAGAGACGAGGAGTTCTGGCTCCTCCTGCAGACATGCACCAGCGGCTGCTGGGCTCGTCCCTGGG CCTCGCCCCCGCGCGGGGGCTCTGAATGCCTGCCGCCGCCCCCATGAGAGCGCCGGCCTGGGCTCCCGCC CCTAAGCCTCTGCTCGCGGAGACTGAGCCATGTGGGCCTGGGGCTGGGCCGCTGCAGCGCTCCTCTGGCT ACAGACTGCAGGAGCCGGGGCGCGGCAGGAGCTCAAGAAGTCTCGGCAGCTGTTTGCGCGTGTGGATTCC CCCAATATTACCACGTCCAACCGTGAGGGGTTCCCAGGCTCCGTCAAGCCCCCGGAAGCCTCTGGACCTG AGCTCTCAGATGCCCACATGACGTGGTTGAACTTTGTCCGACGGCCAGATGATGGGTCCTCTAGAAAACG GTGTCGTGGCCGGGACAAGAAGTCGCGAGGCCTCTCAGGTCTCCCAGGGCCCCCAGGACCTCCTGGCCCT CCTGGTCCCCCTGGCTCCCCTGGTGTGGACGTTACCCCAGAGGCCTTACTGCAGGAATTTCAGGAGATAC TGAAAGAGGCCACAGAACTTCGATTCTCAGGGCTACCAGACACATTGTTACCCCAGGAACCCAGCCAACG GCTGGTGGTTGAGGCCTTCTACTGCCGTTTGAAAGGCCCTGTGCTGGTGGACAAGAAGACTCTGGTGGAA CTGCAAGGATTCCAAGCTCCTACTACTCAGGGCGCCTTCCTGCGGGGATCTGGCCTGAGCCTGTCCTTGG GCCGATTCACAGCCCCAGTCTCTGCCATCTTCCAGTTTTCTGCCAGCCTGCACGTGGACCACAGTGAGCT GCAGGGCAGAGGCCGGTTGCGTACCCGGGATATGGTCCGTGTTCTCATCTGTATTGAGTCCTTGTGTCAT CGTCATACGTCCCTGGAGGCTGTATCAGGTCTGGAGAGCAACAGCAGGGTCTTCACAGTGCAGGTTCAGG GGCTGCTGCATCTACAGTCTGGACAGTATGTCTCTGTGTTCGTGGACAACAGTTCTGGGGCAGTCCTCAC CATCCAGAACACTTCCAGCTTCTCGGGAATGCTTTTGGGTACCTAGCGGAGCTGAAGAAACGATTGTGGA TTGAGGAGCCAACACCTTGCTTCTTAGAGGAGCTGAAAAGGACTACTCCCCTTTTAATAGTTTTCATAGC AATAAAGAACTCCAAACTTCTTCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3966551 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3966551 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19482 same embryo
	EMAGE:19481 same embryo
	EMAGE:19484 same embryo
	EMAGE:19483 same embryo
	EMAGE:19485 same embryo
	EurExpress:euxassay_005009 same experiment
	MGI:4824703 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source