EMAGE mouse gene expression spatial database

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EMAGE:19489

Ptgs1 prostaglandin-endoperoxide synthase 1 ( MGI:97797)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004892. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004892_01	euxassay_004892_02	euxassay_004892_03	euxassay_004892_04

euxassay_004892_05	euxassay_004892_06	euxassay_004892_07	euxassay_004892_08	euxassay_004892_09

euxassay_004892_10	euxassay_004892_11	euxassay_004892_12	euxassay_004892_13	euxassay_004892_14

euxassay_004892_15	euxassay_004892_16	euxassay_004892_17	euxassay_004892_18	euxassay_004892_19

euxassay_004892_20	euxassay_004892_21	euxassay_004892_22	euxassay_004892_23	euxassay_004892_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19489_wholemount_strong.wlz

19489_wholemount_moderate.wlz

19489_wholemount_weak.wlz

19489_wholemount_possible.wlz

19489_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19489_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cornea

strong

regional

strong expression: see section 05 06 24 moderate expression: see section 23 weak expression: see section 04

esophagus

moderate

regional

moderate expression: see section 08 09 10 11

liver left lobe

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

strong

spotted

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

nucleus pulposus

strong

regional

strong expression: see section 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6889
Entity Detected:	Ptgs1, prostaglandin-endoperoxide synthase 1 ( MGI:97797)
Sequence:	sense strand is shown >T6889 GTTGCACCCGAGGAGCCAGCCGTTGGCATTGCACATCCATCCACTCCCAGAGTCATGAGTCGAAGGAGTC TCTCGCTCTGGTTTCCCCTGCTGCTGCTCCTGCTGCTGCCGCCGACACCCTCGGTCCTGCTCGCAGATCC TGGGGTGCCCTCACCAGTCAATCCCTGTTGTTACTATCCGTGCCAGAACCAGGGTGTCTGTGTCCGCTTT GGCCTCGACAACTACCAGTGTGATTGTACTCGCACGGGCTACTCAGGCCCCAACTGTACCATCCCTGAGA TCTGGACCTGGCTTCGGAATTCTCTGCGGCCCAGCCCCTCGTTCACCCATTTCCTGCTGACACATGGATA CTGGCTCTGGGAATTTGTGAATGCCACCTTCATCCGAGAAGTACTCATGCGCCTGGTACTCACAGTGCGG TCCAACCTTATCCCCAGCCCTCCGACCTACAACTCAGCGCATGACTACATCAGCTGGGAGTCCTTCTCCA ATGTGAGCTACTATACTCGCATTCTGCCCTCTGTACCCAAAGACTGCCCCACACCCATGGGGACCAAAGG GAAGAAACAGTTACCAGATGTTCAGCTTCTGGCCCAACAGCTGCTGCTGAGAAGGGAGTTCATTCCTGCC CCCCAGGGCACCAACATCCTGTTTGCCTTCTTTGCACAACACTTCACCCACCAGTTCTTCAAGACCTCTG GAAAGATGGGTCCTGGCTTTACCAAGGCCTTAGGCCACGGGGTAGACCTTGGCCACATTTATGGAGATAA TCTGGAACGACAGTATCACCTGCGGCTCTTCAAGGATGGGAAACTTAAGTACCAGGTGCTGGACGGAGAG GTGTACCCACCTTCCGTGGAACAGGCGTCCGTGTTGATGCGCTACCCACCAGGTGTCCCGCCTGAAAGGC AGATGGCTGTGGGCCAGGAGGTGTTTGGGTTGCTTCCGGGGCTGATGCTCTTCTCCACGATCTGGCTTCG TGAACATAACCGCGTGTGCGACCTGCTGAAGGAGGAGCATCCCACGTGGGATGATGAGCAGCTCTTCCAG ACCACTCGCCTCATCCTTATAGGAGAAACCATCAAAATTGTCATTGAGGAGTATGTGCAGCACTTGAGTG GCTATTTCCTGCAGCTCAAGTTTGACCCGGAGCTGCTGTTCCGAGCCCAGTTCCAATATCGAAACCGCAT CGCCATGGAATTTAACCATCTCTATCACTGGCATCCACTCATGCCCAACTCCTTCCAAGTGGGCTCACAA GAGTACAGCTACGAGCAGTTTTTATTTAACACTTCTATGCTGGTGGACTATGGGGTTGAGGCACTGGTGG ATGCCTTCTCTCGCCAGAGGGCTGGCCGGATTGGTGGAGGTAGGAACTTTGACTATCATGTTCTGCATGT GGCTGTGGATGTCATCAAGGAGTCCCGAGAGATGCGCCTACAGCCCTTCAATGAATACCGAAAGAGGTTT GGCTTGAAGCCTTACACCTCTTTCCAGGAGCTCACAGGAGAGAAGGAGATGGCTGCTGAGTTGGAGGAGC TGTACGGTGACATCGATGCTTTAGAGTTCTACCCGGGGTTGCTGCTGGAGAAGTGCCAGCCCAACTCCAT CTTTGGAGAAAGTATGATAGAGATGGGGGCTCCCTTTTCCCTCAAGGGCCTCCTAGGGAATCCCATCTGT TCCCCAGAGTACTGGAAACCCAGCACGTTCGGTGGTGACGTGGGCTTCAACCTTGTCAACACAGCCTCAC TGAAGAAACTGGTCTGCCTCAACACCAAGACCTGCCCCTATGTTTCCTTCCGTGTGCCAGATTACCCTGG AGATGACGGGTCTGTCTTAGTGAGACGCTCCACTGAGCTCTGAGGGAGCTGGAAAGCAGCCTCTGGAGGG AGGAGTTTTGTTCCTGATGAAGACAGTCCTTGATGTGGGTTTTCGTGGCTTGGCATTGTGAGAGCTGATG CTCACATTTGAAACTTTGGGTCTTACCCTTGCCTAGAATATTGTGATTTTGCCACTTTCGGATGTTGAAT TCTTTGTTAACTAAGAAAGTTAGAAGTGGTTTTGTCTGCCTCCTCAGAACTTGGCTCTTTGTTGGCAACT CAGAAAGTCAGATTTCTGGTTGATTTGGAATATAGGCTTAAAACTTTATATTATAGGGTAGGGTGTGGTT GCACACACCTTAATCCCAGCACTTGGAAGGCAGAGGCAGTTGGATCTCTGGGAGTTTGAGGCCAGTTTGG CCTATATAGTGAGTTCTAGGCCAGCCATGGATGCATAGTGAGACTCTTTCTCAAAACAAACAAACAAACA AACAAACAAACAACTTTTAGAATGTAGAATTCCGTAAAAAAAAAAAATCCCTTGAAAGTTAATGGGGTCC TCAATTTTCTTCCTAGAATTTGGAGGCCTCTTCAGAATGTTGACTATCTGACAGGTGACTCAGAAGGTCC TGTTCCTGGTCAATGATCTATAACATGGGCCAAAACATTCCCAACTTGAATGTCTAGAATGTGGAATTGG TTCATTTTCCTGTTCAGTGAAATGGACACAGAACAAAAGAACCCAGTGTCCAGCAAGAATTGCCTTGCCC AAACCTACGTCTACGCCAAAGGTCAAGGCAGTAAGGTGTTCTTGGGAGCCACACTTAGACTCTTTCCAAA GATGTGGAGGGAACAGATGGACTCATCTATGATCTTGGTTGGAAACCACCACAGTTCTATCCCCATCCAG ATCTTTGCTTGTGGCAGCTGTTTCTCATGAAGCTAATAAAATTCTTTCTCCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3968101 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3968101 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19492 same embryo
	EMAGE:19491 same embryo
	EMAGE:19490 same embryo
	EMAGE:19488 same embryo
	EMAGE:19487 same embryo
	EurExpress:euxassay_004892 same experiment
	MGI:4827510 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source