EMAGE mouse gene expression spatial database

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EMAGE:19490

Gfod2 glucose-fructose oxidoreductase domain containing 2 ( MGI:1917825)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004913. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004913_01	euxassay_004913_02	euxassay_004913_03	euxassay_004913_04

euxassay_004913_05	euxassay_004913_06	euxassay_004913_07	euxassay_004913_08	euxassay_004913_09

euxassay_004913_10	euxassay_004913_11	euxassay_004913_12	euxassay_004913_13	euxassay_004913_14

euxassay_004913_15	euxassay_004913_16	euxassay_004913_17	euxassay_004913_18	euxassay_004913_19

euxassay_004913_20	euxassay_004913_21	euxassay_004913_22	euxassay_004913_23	euxassay_004913_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19490_wholemount_strong.wlz

19490_wholemount_moderate.wlz

19490_wholemount_weak.wlz

19490_wholemount_possible.wlz

19490_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19490_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tongue

moderate

regional

moderate expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6904
Entity Detected:	Gfod2, glucose-fructose oxidoreductase domain containing 2 ( MGI:1917825)
Sequence:	sense strand is shown >T6904 CCCCTGTTCCTTAGAAACCAGGCGGCGTGTTCCCGGTATCGGTGCTTTGGATTCCGGCGCGCGCGCATCC GCACTAGCCTCCCTCAGGGCAGGCGGACGGCCTCGGAAACCCCTCCGGATCCATGGTCTCTCGTGCAGCT CTGGGCGGGCAATGCCAGCATGCCAGGAGCCATGTTTGATCAGGATGACGGGACGGTCAGCTGCATCCCG GGGACCCTTGTAAGGACATGAGTGCTTGAGCAGAGGCCAGGATGAAGCTGCTCCCAGGAGTCGGTGTGTT CGGGACTGGCAGCTCAGCCCGGGTCCTGGTCCCGCTGCTGAGGGCAGAGGGCTTCACCGTGGAGGCCCTG TGGGGGAAGACTGAGGAAGAGGCAAAGCAGCTGGCCGAGGAGATGAACATCACCTTCTACACCAGCCGCA CAGACGACGTCTTGCTGCATCAAGACGTAGACCTGGTCTGCATCAACATCCCACCTCCGCTCACCCGGCA GATATCCGTGAAGGCTCTAGGAATTGGGAAGAACGTGGTGTGTGAGAAGGCAGCGACATCCATGGACGCC TTCCGGATGGTGACCGCATCTCGCTACTACCCACAGCTGATGAGCTTGGTGGGGAACGTTCTGCGCTTCC TGCCTGCCTTCGTGCGCATGAAACAGCTCATTGCAGAGCACTACGTGGGTGCCGTGATGATCTGCGACGC TCGCATCTACTCCGGGAGCCTGCTCAGCCCCAGCTACGGCTGGATCTGTGACGAGCTTATGGGGGGCGGG GGCCTGCACACCATGGGTACCTATATCGTGGACCTGTTGACCCACTTGACTGGCCAGAAAGCCGAGAAGG TGCACGGGCTCCTCAAGACCTTTGTGAGGCAGAATGCCACCATCCGTGGCATCCGCCATGTCACCAGTGA TGACTTCTGTTTTTTCCAGATGCTCATGGGTGGGGGGGTATGCAGCACAGTGACCCTCAACTTCAACATG CCAGGTGCCTTTGTGCATGAGGTCATGGTAGTGGGCTCCGCAGGGCGCCTTGTTGCCCGGGGAGCTGACC TCTATGGGCAGAAGAACTCTGCTGCACAGGAGGAGCTGCTGGTGAGAGACTCTCTGGCTGTGGGCGCAGG GCTCCCTGAGCAAGGTCCCCAGGATGTCCCACTGCTTTACTTGAAAGGTATGGTCTATATGGTTCAGGCC TTGCGTCAGTCCTTCCAGGGGCAGGGGGATCGCCGTACCTGGGACCGCACCCCTGTCTCTATGGCTGCCT CATTCGAGGACGGACTGTATATGCAGAGCGTGGTAGATGCTATTAAAAGGTCCAGCCGGTCTGGGGAGTG GGAGACTGTGGAGATGCTGGCAGAGGAGCCAGATGCCAACCAGAATCTAAGTGAAACACTTCAGCGGAAC AACCTGTGAACCTGCGCATGCGCGCTTCCTCCCAAGGGCCAGAGGCTCCAGGGAGGGCACTGGGACCTGG GATTGGCTGTAGCAGAGACACGGTGTCGTTTTTTAAATAAATCAGCTTGGGCCAGGGTTAGGAGCCAACT GAAGGTGGCCAAGGAAAACACCCATCCATCCATCATTGATTCCTCAGACTTGCCTGGGGACCCCTAGCTA TGACGCACCTGCTGTCATAGCTTAGGCTAGCAGTGTGTGCAGGATAGAGCTGTAGCCTGACCCCTTGTGG TTCTGCCAGTGAGCAGGGCCAATGAAGATCAATATGGGCTCTTCAGGCTGAGTTGTGGTCTCACAGGATG AGAAAACCCAACAGGAACTAGCTTGGTTGAGTCCTTAGGGTAGATGGCAAGAAACCTTGCCTGTTGTGGA CCAGGGCATCTCAGGACTGGAGAAGGCGGTGACAGGTCATCCCTTAGGGATCTTCCCCCACCCTCCCATT CGATCTGATCTTTTCAGCCCCCTTGCAAGCCCTTCCAAGAGAAAAGGAGGTTTCTGTGTTATACATAACT CTGGGGGGGGGGGGGGCAACTTGGCAGAGAGAAGACAAAGGGGAACTGTAGAACATCTGAGAAATGTCAG TAAATAAGCCAGAAAAGAACTGAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3969153 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3969153 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19492 same embryo
	EMAGE:19489 same embryo
	EMAGE:19491 same embryo
	EMAGE:19488 same embryo
	EMAGE:19487 same embryo
	EurExpress:euxassay_004913 same experiment
	MGI:4825045 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source