EMAGE mouse gene expression spatial database

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EMAGE:19505

Acvrl1 activin A receptor, type II-like 1 ( MGI:1338946)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004915. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004915_01	euxassay_004915_02	euxassay_004915_03	euxassay_004915_04

euxassay_004915_05	euxassay_004915_06	euxassay_004915_07	euxassay_004915_08	euxassay_004915_09

euxassay_004915_10	euxassay_004915_11	euxassay_004915_12	euxassay_004915_13	euxassay_004915_14

euxassay_004915_15	euxassay_004915_16	euxassay_004915_17	euxassay_004915_18	euxassay_004915_19

euxassay_004915_20	euxassay_004915_21	euxassay_004915_22	euxassay_004915_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19505_wholemount_strong.wlz

19505_wholemount_moderate.wlz

19505_wholemount_weak.wlz

19505_wholemount_possible.wlz

19505_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19505_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6932
Entity Detected:	Acvrl1, activin A receptor, type II-like 1 ( MGI:1338946)
Sequence:	sense strand is shown >T6932 CACGCGTCCGTGAGCACAGCCCCTTCCCAGTCCCCGGAGGCCGCCGCGCCAGCCGCGCAGATCGAGACCT TTCCCGGACGCACAGGCCCGCTCTGGACGTGAGACCCCGGCCGCCTCCGGCAAGGAGAGGCGGGGGTCGA GTCGCCCTGTCCAAAGATCTCACCACATCTCTTCTCCTATCTCCAAGGACCATGACCTTGGGGAGCTTCA GAAGGGGCCTTTTGATGCTGTCGGTGGCCTTGGGCCTAACCAGGGGAGACTTGGCGAAGCCTTCCAAGCT GGTGAACTGCACTTGTGAGAGCCCACACTGCAAGAGACCATTCTGCCAGGGGTCATGGTGCACAGTGGTG CTGGTTCGAGAGCAGGGCAGGCACCCCCAGGTCTATCGGGGCTGCGGGAGCCTGAACCAGGAGCTCTGCT TGGGACGTCCCACAGAGTTTCTGAACCATCACTGCTGCTATAGATCCTTCTGCAACCACAACGTGTCTCT GATGCTGGAGGCCACCCAAACTCCTTCGGAGGAGCCAGAAGTTGATGCCTATCTGCCTCTGATCCTGGGT CCTGTGCTGGCCTTGCCGGTCCTGGTGGCCCTGGGTGCTCTGGGCTTGTGGCGTGTCCGGCGGAGGCAGG AGAAGCAGCGGGATTTGCACAGTGACCTGGGCGAGTCCAGTCTCATCCTGAAGGCATCTGAACAGGCAGA CAGCATGTTGGGGGACTTCCTGGACAGCGACTGTACTACGGGCAGCGGCTCGGGGCTCCCCTTCTTGGTG CAGAGGACGGTAGCTCGGCAGGTTGCGCTGGTAGAGTGTGTGGGAAAGGGCCGATATGGTGAGGTGTGGC GCGGTTCGTGGCATGGCGAGAGTGTGGCGGTCAAGATTTTCTCCTCACGAGATGAGCAGTCCTGGTTCCG GGAGACGGAGATCTACAACACAGTTCTGCTTAGACACGACAACATCCTAGGCTTCATCGCCTCCGACATG ACTTCGCGGAACTCGAGCACGCAGCTGTGGCTCATCACCCACTACCATGAACACGGCTCCCTCTATGACT TTCTGCAGAGGCAGACGCTGGAGCCCCAGTTGGCCCTGAGGCTAGCTGTGTCCGCGGCCTGCGGCCTGGC GCACCTACATGTGGAGATCTTTGGCACTCAAGGCAAACCAGCCATTGCCCATCGTGACCTCAAGAGTCGC AATGTGCTGGTCAAGAGTAACTTGCAGTGTTGCATTGCAGACCTGGGACTGGCTGTGATGCACTCACAAA GCAGCGATTACCTGGATATCGGCAACAACCCCCGAGTGGGTACCAAAAGATACATGGCACCCGAGGTGCT GGATGAGCACATCCGCACAGACTGCTTTGAGTCGTACAAGTGGACAGACATCTGGGCCTTTGGCCTAGTG CTATGGGAGATCGCCCGGCGGACCATCATCAATGGCATTGTGGAGGATTACAAGCCACCTTTCTATGACA TGGTACCCAATGACCCCAGTTTTGAGGACATGAAAAAGGTGGTGTGCGTTGACCAGCAGACACCCACCAT CCCTAACCGGCTGGCTGCAGATCCGGTCCTCTCCGGGCTGGCCCAGATGATGAGAGAGTGCTGGTACCCC AACCCCTCTGCTCGCCTCACCGCACTGCGCATAAAGAAGACATTGCAGAAGCTCAGTCACAATCCAGAGA AGCCCAAAGTGATTCACTAGCCCAGGGCCACCAGGCTTCCTCTGCCTAAAGTGTGTGCTGGGGCAGAAGA CATAGCCTGTCTGGGTAGAGGGAGTGAAGAGAGTGTGCACGCTGCCCTGTGTGTGCCTGCTCAGCTTGGC TCCCAGCCCATCCAGCCAAAAATACAGCTGAGCTGAAATTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3982004 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3982004 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19506 same embryo
	EMAGE:19508 same embryo
	EMAGE:19507 same embryo
	EMAGE:19510 same embryo
	EMAGE:19509 same embryo
	EurExpress:euxassay_004915 same experiment
	MGI:4822966 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source