EMAGE mouse gene expression spatial database

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EMAGE:19545

Sass6 spindle assembly 6 homolog (C. elegans) ( MGI:1920026)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004943. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004943_01	euxassay_004943_02	euxassay_004943_03	euxassay_004943_04

euxassay_004943_05	euxassay_004943_06	euxassay_004943_07	euxassay_004943_08	euxassay_004943_09

euxassay_004943_10	euxassay_004943_11	euxassay_004943_12	euxassay_004943_13	euxassay_004943_14

euxassay_004943_15	euxassay_004943_16	euxassay_004943_17	euxassay_004943_18	euxassay_004943_19

euxassay_004943_20	euxassay_004943_21	euxassay_004943_22	euxassay_004943_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19545_wholemount_strong.wlz

19545_wholemount_moderate.wlz

19545_wholemount_weak.wlz

19545_wholemount_possible.wlz

19545_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19545_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 08 09 10 12 13 14 16 17 18 20 weak expression: see section 04 05 06 07 11 15 19

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 08 09 10 12 13 14 16 17 18 20 21 weak expression: see section 04 05 06 07 11 15 19

pons ventricular layer

moderate

regional

moderate expression: see section 08 09 10 12 13 14 16 17 18 20 weak expression: see section 06 07 11 15 19

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

retina

moderate

regional

moderate expression: see section 01 02 21 22 weak expression: see section 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8000
Entity Detected:	Sass6, spindle assembly 6 homolog (C. elegans) ( MGI:1920026)
Sequence:	sense strand is shown >T8000 CTGAGCGCCAAACTCAAACTCATCGGGACCGGCTCTTCTTTGCAGCCGGGACCGAAGGCCGGGCCTACTG GGCTTCTTCACCCTCCCGTCGGGGCAGCGGTCCGACATGAGTCAAGTTCTGTTCCAGCAGCTTGTTCCCT TGCTAGTGAAATGCAAAGACTGCGAGGAGAGGAGAGGGAGCGTTAGAGTGAGCATTGAGCTGCAGTCCCT TTCGAATCCAGTACACAGGAAGGATTTAGTCATCCGTCTGACTGATGATACAGATCCGTTTTTTCTGTAT AACCTTGTTATATCTGAGGAAGATTTTCAAAGTTTAAAATTGCAGCAAGGTCTCCTGGTGGACTTCTTAG CTTTCCCACAGAAGTTTATAGACCTCCTTCAGCAGTGTATGCAAGAACACGCGAAAGAGACTCCAAGGTT CCTGCTGCAGCTTCTTTCTTCAGCCACTCTTTTGGAGAACTCACCGGTCCTTTTAAACGTAGTGGAGACA AATCCTTTTAAGCATCTTATTCACCTGTCACTAAAGCTTTTACCTGGAAATGATGTAGAAATAAAGAAAT TTCTAGCAGGATGTTTGAAATGTAGCAAGGAAGAAAAATTATCACTGACTAGATCACTAGATGATGTTAC CAGGCAACTGCACATTACACAAGAGACGTTGTCGGAAAAAATGCAAGAATTAGATAAGCTGCGGAGTGAA TGGGCCTCGCACACAGCATCGCTGACGAATAAGCACTCTCAGGAGTTAACAGCTGAGAAGGAGAAGGCCT TGCAGACCCAAGTTCAGTGCCAACAGCAGCACGAGCAACAGAAAAAGGAACTGGAAACCCTCCATCAACG GAATATCCACCAGCTACAAAGCAGATTGTCCGAGTTAGAGGCAGCTAATAAAGAGCTCACCGAGAGGAAG TATAAAGGAGACTCGACTGTCCGAGAGCTAAAGGCGAAGCTGGCTGGCGTGGAAGAGGAGCTGCAGCGGG CCAAGCAAGAGGTCCTTTCTCTGAGAAGAGAGAATTGTACTCTGGATACTGAATGCCACGAGAAAGAAAA GCACATCAACCAGCTACAAACAAAAGTGGCCGTTTTAGAACAGGAGATCAAAGATAAAGACCAGCTTGTT CTGAGAACAAAAGAAGCATTCGATACAATCCAAGAGCAAAAGGTGGCTCTAGAAGAAAATGGTGAGAAAA ATCAGATACAACTGGGAAAACTTGAAGCTACAATAAAATCATTATCAGCAGAACTTCTAAAGGCAAATGA GATCATCAAGAAGTTACAAGGAGATCTTAAAACTCTGATGGGTAAATTGAAACTGAAGAATACAGTTACT ATTCAGCAAGAAAAACTATTGGCTGAGAAAGAAGAAATGCTACAAAAGGAGCGAAAGGAATCACAGGACG CTGGGCAGTTTCTTCGTGCCAAAGAGCAAGAGGTATGCAGATTACAGGAACAATTAGAAACTACAGTTCA GAAACTCGAAGAAAGTAAACAACTTTTGAAAAATAATGAAAAATTAATCACGTGGCTAAACAAAGAGCTA AATGAAAATCAGCTGGTAAGAAAACAGGACACATTGGGAACCTCTGCCACCCCACATTCTACTAGCAACA GCACCATCAGAAGTGGGCTCTCTCCCAATCTGAATGTGGTTGATAGACTAAATTACCCAAGCTGTGGAAT TGGCTATCCTGTCTCCTCTGCATTGACATTCCAGAATGCCTTTCCTCATGTAGTAGCTGCCAAAAACACC AGCCACCCTATCTCTGGACCAAAGGTTCACTTTAACCTGCAGCTTACAAAACCAAGCGCTTCGATAGATG GGCAGCCGGGAGCCGCTGTTGACAGGCCTTGTTCAAATGATAAGGAAAATGGTGAAACTTTAGGATTGGA ATCCAAATACTTGAAGAGAAGAGAAGCTAGCATTCCTTTACGCGGACTTAGCCAGAATCTGTTGAGTGAC TCAGACCATCAGAAAGACGGCATGCTGGGAGCGTTCCAGCTATCTTCCAAACCCACCGTTCTCCCCTCCT CGTCTTCAGCCTACTTCCCTGGGCAGTTACCAAGTAGTTAATCCAACACCTTTTACTGCTCACCGACAGT TTAGGAATTCAAGTGATTTAACAGACTATTGATAACAAAATCAATTTTTTTCTGGTTCCTGTTAGGTCAT TTGGATCCTTTATAATACTGCTGCCATTTACAAATTTAGGAAATTGATAAGGATGCTGAGTAAATGGTAT TTTTTTCCTGTGAACAAAACAGAATGTTTCAAAAAGTCACTGGATTGTATCAGATTCTTACCAGTTGGTC TGCTTAGGGACACTTTATCAAGATACACCTGCAGGGAATATAAACTGAAGACTTGTGATTCATTCGATCA TTTTTATTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30024366 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30024366 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19546 same embryo
	EMAGE:19547 same embryo
	EMAGE:19544 same embryo
	EMAGE:19548 same embryo
	EurExpress:euxassay_004943 same experiment
	MGI:4827874 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source