EMAGE mouse gene expression spatial database

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EMAGE:19548

Usp15 ubiquitin specific peptidase 15 ( MGI:101857)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005002. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005002_01	euxassay_005002_02	euxassay_005002_03	euxassay_005002_04

euxassay_005002_05	euxassay_005002_06	euxassay_005002_07	euxassay_005002_08	euxassay_005002_09

euxassay_005002_10	euxassay_005002_11	euxassay_005002_12	euxassay_005002_13	euxassay_005002_14

euxassay_005002_15	euxassay_005002_16	euxassay_005002_17	euxassay_005002_18	euxassay_005002_19

euxassay_005002_20	euxassay_005002_21	euxassay_005002_22	euxassay_005002_23	euxassay_005002_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19548_wholemount_strong.wlz

19548_wholemount_moderate.wlz

19548_wholemount_weak.wlz

19548_wholemount_possible.wlz

19548_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19548_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 11 moderate expression: see section 12 13 14 15

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 16 17 18 19 weak expression: see section 10

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 04 05

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 18 weak expression: see section 19

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 17 18 19 20 21 22

trigeminal v nerve

strong

regional

strong expression: see section 09

spinal cord

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 15 16

cervical ganglion

moderate

regional

moderate expression: see section 09 17

thoracic ganglion

moderate

regional

moderate expression: see section 10

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 14 15 16 17 18

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 11 12 14 15

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7990
Entity Detected:	Usp15, ubiquitin specific peptidase 15 ( MGI:101857)
Sequence:	sense strand is shown >T7990 GCTACGGCTAGTGGAAGAAGATGGCGGAAGGCGGAGCGGCGGACCTGGACACCCAGCGTTCTGACATCGC GACGCTGCTCAAAACCTCGCTCCGGAAAGGGGACACCTGGTATCTAGTAGATAGTCGGTGGTTCAAACAG TGGAAAAAATATGTTGGCTTTGACAGCTGGGACAAATACCAGATGGGAGATCAAAATGTCTATCCTGGAC CCATCGATAACTCTGGACTTCTCAAAGATGGCGACGCGCAGTCACTTAAGGAGCACCTTATTGATGAGTT GGATTACATACTCTTGCCAACTGAGGGCTGGAATAAACTTGTCAGCTGGTACACACTGATGGAGGGCCAG GAGCCAATAGCAAGAAAGGTGGTAGAACAGGGTATGTTTGTAAAGCACTGCAAAGTAGAAGTCTACCTCA CAGAACTGAAGCTCTGTGAGAACGGGAACATGAACAATGTTGTAACTCGGAGGTTTAGCAAAGCTGACAC AATAGATACGATTGAGAAGGAAATAAGAAAAATCTTCAATATTCCAGATGAGAAGGAGGCCAGACTGTGG AACAAGTATGTGAGCAACACATTTGAACCACTGAATAAGCCAGACAGCACCATCCAGGATGCCGGCCTAT ACCAAGGACAGGTGTTAGTGATAGAGCAAAAAAATGAAGATGGAACGTGGCCCAGGGGTCCCTCTACTCC TAAGTCCCCAGGTGCATCCAATTTTTCAACTTTACCAAAGATCTCTCCTTCATCTCTATCAAATAATTAT AACAACATCAACAACAGAAATGTGAAAAACTCAAATTATTGTCTCCCGTCATATACTGCTTATAAGAACT ATGATTATTCAGAACCTGGAAGAAACAATGAACAGCCAGGCCTGTGTGGCTTAAGTAACTTGGGAAATAC GTGTTTCATGAACTCAGCCATTCAGTGTTTGAGCAACACACCTCCACTTACTGAATATTTCCTCAATGAT AAGTACCAAGAAGAGCTGAATTTTGACAATCCCTTAGGAATGAGAGGTGAAATAGCTAAATCATACGCTG AACTCATCAAGCAGATGTGGTCTGGAAAGTTTAGCTACGTCACTCCAAGAGCATTCAAGACACAGGTAGG GCGCTTCGCACCTCAGTTCTCTGGATATCAGCAGCAGGACTGCCAAGAACTACTGGCTTTCCTGTTGGAT GGACTGCACGAGGACCTGAACAGAATTAGGAAAAAACCCTATATCCAGTTAAAAGATGCTGATGGCAGGC CAGACAAGGTGGTTGCTGAAGAAGCCTGGGAGAACCATTTAAAGCGAAATGATTCCATCATAGTAGACAT ATTCCATGGCCTTTTCAAGTCTACGTTAGTGTGTCCTGAGTGTGCTAAGATCTCGGTGACCTTTGACCCT TTCTGTTACTTGACTCTTCCATTGCCTATGAAAAAAGAACGTAGCTTGGAAGTTTATTTAGTTAGAATGG ATCCACTTGCTAAACCTATGCAATACAAAGTTATTGTGCCCAAAATTGGAAATATACTAGATCTTTGTAC AGCGCTGTCTGCTTTGTCGGGAGTACCTGCAGATAAGATGATAGTCACTGACATATACAATCATAGATTT CACAGGATATTTGCCGTGGATGAAAACCTGAGTAGTATTATGGAGCGGGATGATATTTACGTGTTTGAAA TTAACATCAACAGGGCAGAAGACACAGAGCATGTAGTCATTCCTGTTTGCCTAAGAGAAAAATTCAGACA CTCAAGTTACACGCACCACACTGGCTCTTCACTGTTCGGCCAGCCTTTTCTTATGGCTATACCACGAAAC AATACTGAAGATAAACTCTATAATCTTCTACTCTTGAGAATGTGCCGGTATGTCAAAATGTCTACTGAAA CTGAGGAAACAGATGGACACCTACGTTGCTGTGAGGACCAGAATATTAATGGGAATGGGCCAAATGGCCT ACATGAAGAGGGTTCACCAAGTGAGATGGAAACCGACGAGCCAGGTGACGAGTCCAGCCAGGATCAAGAG CTTCCCTCAGAGAACGAGAACAGTCAGTCTGAAGATTCAGTCGGAGGAGATAACGATTCTGAAAATGGAT TGTGCACTGAAGAAACATGCAAAGGTCAACTTACGGGACACAAAAAGCGCTTGTTTACATTCCAGTTCAA CAACTTAGGCAACAATGACATCAACTACATCAAAGACGACACCAGTCACATCCGATTCGATGACAGGCAG CTTCGGCTGGACGAAAGATCTTTTCTCGCTTTGGACTGGGACCCTGACTTGAAGAAGAGATACTTTGATG AGAATGCTGCAGAGGATTTTGAAAAACATGAAAGTGTGGAATACAAACCTCCAAAAAGGCCCTTTGTGAA GTTAAAAGACTGCATTGAGCTCTTCACCACCAAGGAGGAGTTGGGTGCTGAGGACCCCTGGTATTGTCCA AATTGTAAAGAGCATCAGCAAGCCACAAAGAAATTAGATCTGTGGTCCCTGCCTCCAGTTCTTGTGGTGC ATCTCAAGAGATTCTCCTATAGTCGCTACATGAGAGACAAGCTAGATACATTAGTCGATTTTCCCATCAG TGACTTGGATATGTCAGAATTCCTAATTAACCCAAATGCAGGTCCTTGCCGCTATAATTTGATTGCTGTT TCCAACCACTATGGAGGGATGGGAGGAGGACATTATACTGCTTTTGCAAAAAACAAAGATGATGGGAAGT GGTACTATTTCGATGACAGTAGCGTCTCCACTGCATCTGAAGACCAAATCGTGTCCAAGGCAGCGTACGT GCTCTTCTACCAGAGACAGGACACTTTCAGTGGGACTGGCTTCTTCCCTCTTGACCGAGAAACTAAAGGT GCTTCAGCTGCCACAGGTATCCCCCTGGAAAGTGACGAAGACAGCAATGATAATGACAATGACCTAGAAA ATGAAAACTGTATGCACACTAATTAACGAAAGTGCAAGAAGCCATAGGAGAGACTTTCCTGCTGGTGGTG TCTATGGAGATGAAGTTATCACCACATGAAACAGAAGTCTGAGATGAGGAGTTTCAGATACCCGAATGTA AATCCTTTATCAGATTTTAACTTGTGCAGTACTTGAAGTGAAACACAATGAAAACTTTAACAGAAATTGT CTCTTAATACATTTACAGTCTTGTATTTACAAGCTAAATATATATAGGAAATCACAAATAAATCCCTTTT AAGTTTGAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30022307 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30022307 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19545 same embryo
	EMAGE:19546 same embryo
	EMAGE:19547 same embryo
	EMAGE:19544 same embryo
	EurExpress:euxassay_005002 same experiment
	MGI:4829128 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source