EMAGE mouse gene expression spatial database

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EMAGE:19556

C80913 expressed sequence C80913 ( MGI:1342294)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002068. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002068_01	euxassay_002068_02	euxassay_002068_03	euxassay_002068_04

euxassay_002068_05	euxassay_002068_06	euxassay_002068_07	euxassay_002068_08	euxassay_002068_09

euxassay_002068_10	euxassay_002068_11	euxassay_002068_12	euxassay_002068_13	euxassay_002068_14

euxassay_002068_15	euxassay_002068_16	euxassay_002068_17	euxassay_002068_18	euxassay_002068_19

euxassay_002068_20	euxassay_002068_21	euxassay_002068_22	euxassay_002068_23	euxassay_002068_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19556_wholemount_strong.wlz

19556_wholemount_moderate.wlz

19556_wholemount_weak.wlz

19556_wholemount_possible.wlz

19556_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19556_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 10 11 12 13

brain

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

spinal cord

weak

homogeneous

weak expression: see section 09 10 11 12 13 14 15 16 17 18

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5476
Entity Detected:	C80913, expressed sequence C80913 ( MGI:1342294)
Sequence:	sense strand is shown >T5476 CGGACACGTGGGCGGACGCGTGGGCGGACGCGTGGGCGGACACGTGGGCGCCGCTCCGCTGCGCGCACCA GAGGTGGCGCGGCTTCGCGAGGAGCAGGAAAAGGTGGTCACTAACTGCCAAGAGAAAATCCAGCATTGGG AGAAAGTAGACAATGACTACAGTGCTCTTCAAGAGAGGCTGAGGACCTTGCCTGACAAGCTGTCTTACGA CGTTATGGTACCATTCGGCCCTCTTGCCTTCATGCCAGGAAAACTAGTCCACACTAATGAAGTCACTGTT TTACTTGGCGACAACTGGTTTGCAAAGTGCTCAGCCAAGCAGGCTGTGGGTTTAGTTGAGCATCGGAAAG AACATGTAAGAAAAACAATAGATGATTTTAAAAAAGTGTTGAAAAATTTTGAATCCAGAGTTGAATTCAC AGAAGATTTGCAGAAGATGAGTGATGCTGCAGGTGATTTTGTTGACATAAGAGAAGAAATTAAAAGTGAC TTTGAATTTAAAGGAAAACAACGAATTGCTCACAAACCTCATTCCAAACCAAAAACTTCAGATATTTTTG AAGCAGATTTTGAAAATGGTGTAAAACCCAAGGATACATTTGATGCTGATGAACTGTGGGCTCGACTTGA AGAGCTGGAGAGACAAGAAGAACTGCTGGGTGAACTTGAAAGTAAACCTGATACTGTGATTGCAAATGGA GAAGACAGAGTGTCTTCTGAGGAGGAAAAGGAAGGTGCAGACACAGGTGTGAATGTGGTGTCTCCAGTGA CAGACTCCTCTGCCGCCAGCAGCTGTAAAAGGCGTGCTGGAAATGCAGGACTCCCTAATGGTCAAGTCAA TAGTCTGAACTATTCAGTGAATGGCTCAAATTCTTACCACAGCAACAAAGATGATGATGAAGAAGAAGAT GACGATGATGATGATGATGATGAAGATGACGATAATGAAAGTGACCATGCTATTTCAGCAGATAATTCTA TTCCAACAATATATTTCTCTCATACTGTTGAACCTAAAAGGGTTCGAATAAATACTGGGAAAAATACCAC TTTAAAGTTCAGTGAGAAGAAAGAGGAAGCCAAACGGAAACGAAAGAGTGGTGCCGGCAGTCACGCTACC CACGAGCTGCCTGCCATCAAGAGTCCTGCTGATATTTACAGAGTCTTCGTGGATGTTGTGAATGGAGAAT ATGTTCCTCACAAGTCAATCCTCAAGTCTCGCAGCCGAGAGAACAGCGTGTGCAGCGACACAAGTGAGAG CAGCGCTGCCGATGTGGAGGACCGGCGGGGGCTCCTGCGGAGCACCAGCTCTGAGGAGGCCGTGGCTACC GAGGCCGGCGGGAGCAGTTTGGACGAACTCCAAGAGAACCACCCCAAGAAGCCTTTGCCCTCAGGAGTGT CTGAGGCTTTTTCTGGAACTGTAATAGAAAAAGAATTTTTATCACCCTCCCTAGCACCATACTCAGCCAT TGCTCACCATGCCCTCCCCACCATTCCAGAGCGAAAAGAAGTTCCATCAGAAGTATCAGAGGAACCCACA AAGAGGGTGTCAAAGTTCAGAGCTGCCCGATTGCAGCAGAGAAGCTAGAGGGCTCCGCCTGCAGCTCCAG CTGCGCTTTAGGACATGCGGCGGATTCGGCCATGGAGTTGGGACGGCGTCCTGCTTGGCTGGGCAGAGCT CTCTCACCTTTATCAGTGGCATTGGGAAAGTCAAAGTAAATGTTTGAATGCTGTAATGTTCTGCTGGCTT CGTTAATTCTGTGACTACTGTCTAATTGGCCTTAGGATGCAGGAATAGCGTTTTGAGTTACTTTTCAAAG AATCCTGTCATATATTGTTTGTGTGTTTTCAACTAAACACAGGCAGTTTCGTACCAGCTGTGATGTTGTG CTTTCCATATGGACCATGTTAAAGACACTTTTAAAATCTCAAATAGATTCAACAATTATATTACTTGCTT TTACATTTTAAAGGCACTTTAAAAAAAATCTACATTTCTTGTAGGTTTCACAGCTGTATGGTTCTTTGAA AAATTCTTCCTTTGAATGTCATACTGTAATCTTTAAGGCAAAAAGCTACACAAACCCTTTGGGAAAATTT GACTAGAAGAAGAATATTTTGTACAACTTTTTTTTTTTGGAAGTAAAATTTTTATGAAACTTGGTTTCTA AAATGTTGTGAACTTCCTGGTTTAGATTGTGTATAAAATGTCTTTGATTTGTGTATAGGCCACACCACAG ATTTTCAAAGTTTTCAAGTCCCACGAGCACCTCCTCTCCCCATACACACAGTTGTCATTGGCACCATTCT TTGGGGGGAAAAAGTTTGTCTAGATGCTTTAACACTTTGTTAAAGCACAGGCCTTTTGTTTGTTTGTTTG TTTGTTTTACATTTCAATCTTCCTCACATTTTCATTCTTCAAGGATGCTTTCCTACCAGTAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5374731 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5374731 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19557 same embryo
	EMAGE:19560 same embryo
	EMAGE:19558 same embryo
	EMAGE:19559 same embryo
	EMAGE:19555 same embryo
	EurExpress:euxassay_002068 same experiment
	MGI:4823563 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source