EMAGE mouse gene expression spatial database

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EMAGE:19558

Gtf3a general transcription factor III A ( MGI:1913846)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002074. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002074_01	euxassay_002074_02	euxassay_002074_03	euxassay_002074_04

euxassay_002074_05	euxassay_002074_06	euxassay_002074_07	euxassay_002074_08	euxassay_002074_09

euxassay_002074_10	euxassay_002074_11	euxassay_002074_12	euxassay_002074_13	euxassay_002074_14

euxassay_002074_15	euxassay_002074_16	euxassay_002074_17	euxassay_002074_18	euxassay_002074_19

euxassay_002074_20	euxassay_002074_21	euxassay_002074_22	euxassay_002074_23	euxassay_002074_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19558_wholemount_strong.wlz

19558_wholemount_moderate.wlz

19558_wholemount_weak.wlz

19558_wholemount_possible.wlz

19558_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19558_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 10 11 13 14 moderate expression: see section 12

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 16 moderate expression: see section 18 19

brain

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 08 09 17 18 19 20 21 22 weak expression: see section 07

spinal cord

weak

homogeneous

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 12 13 16 17 weak expression: see section 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5472
Entity Detected:	Gtf3a, general transcription factor III A ( MGI:1913846)
Sequence:	sense strand is shown >T5472 CCACGCGTCCGCCTTCATCAGAGACTACCATCTGAGCCGGCATGTCCTGATTCACACCGGGGAAAAGCCG TTTGTTTGTGCAGATGATGGCTGTAATCAGAAATTCAACACAAAATCAAACTTGAAGAAACACATTGAAC GCAAACATGGAAACCCACAAAAACAGTATGTGTGCAGTTATGAGGGTTGCAAGAAGGCCTTTAAGAAGCA CCAGCAGCTGAGAACCCATCAGTGCCAGCACACCAGCGAGCCACTCTTCAGGTGTACCCACGAGGGATGC GGGAAGCACTTTGCCTCGCCCAGCAGGCTGAAACGGCATGGGAAAGTTCACGAGGGCTACCTGTGTCAAA AGGGATGTTCTTTCATGGGAAAAACGTGGACAGAGCTTCTGAAACACATGAGAGAAGCCCATAAAGAGGA CATAACCTGCAACGTATGTCAGAGGATGTTCAAGCGCAGAGATTACCTTAAGCAGCACATGAAGACTCAC GCCCCGGAAAGGGATGTGTACCGCTGTCCGCGGCAAGGCTGCGGAAGAACCTACACAACCGTGTTCAACC TGCAGAGCCACATTCTCTCCTTCCACGAGGAAAAGCGCCCATTTGTGTGTGAGCACGCTGGCTGTGGCAA GACATTCGCAATGAAACAGAGTCTCATGAGGCACAGTGTCGTGCACGATCCCGACAAGAAGAGGATGAAG CTCAAAGTAAGAGCCCCTCGGGAGAGACGCAGCTTGGCCTCTCGCCTCAGTGGGTACTTCCCTCCTAAGA GGAAACAAGAGCCCGACTACTCCTTGCCTAACGCCAGCGCAGAGTCCAGCAGCAGTCCAGAGGCCCAGCT GCCCCCGCCAGCCACCTTACTCACTGTCTGCTAGGCGGGAAGACTTCAGGGCGGCCGGCAGAGGCTCCTG GCCTGCACTTTTCCTATTAAAGTCACTGACACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5374268 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5374268 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19557 same embryo
	EMAGE:19556 same embryo
	EMAGE:19560 same embryo
	EMAGE:19559 same embryo
	EMAGE:19555 same embryo
	EurExpress:euxassay_002074 same experiment
	MGI:4825279 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source