EMAGE mouse gene expression spatial database

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EMAGE:19561

Gas8 growth arrest specific 8 ( MGI:1202386)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004857. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004857_01	euxassay_004857_02	euxassay_004857_03	euxassay_004857_04

euxassay_004857_05	euxassay_004857_06	euxassay_004857_07	euxassay_004857_08	euxassay_004857_09

euxassay_004857_10	euxassay_004857_11	euxassay_004857_12	euxassay_004857_13	euxassay_004857_14

euxassay_004857_15	euxassay_004857_16	euxassay_004857_17	euxassay_004857_18	euxassay_004857_19

euxassay_004857_20	euxassay_004857_21	euxassay_004857_22	euxassay_004857_23	euxassay_004857_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19561_wholemount_strong.wlz

19561_wholemount_moderate.wlz

19561_wholemount_weak.wlz

19561_wholemount_possible.wlz

19561_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19561_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 07

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 09 20 21

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 10 20

trigeminal v nerve

moderate

regional

moderate expression: see section 09 weak expression: see section 17

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20 21

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 12 14 15 16 17

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 11 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6561
Entity Detected:	Gas8, growth arrest specific 8 ( MGI:1202386)
Sequence:	sense strand is shown >T6561 GATTAGTGGAAGTGCTGGACTGCTGGCACCATGGCACCCAAAAAGAAAGGGAAGAAGGGGAAAGCCAAAG GGACCGCGATTGTTGATGGCGTCGCACCAGAGGACATGACCAAGGAGCAGGTGGAGGAACATGTCGCCCG CATCCGGGAGGAGCTGGACAGGGAGAGAGAGGAGCGTAACTACTTCCAGCTGGAGCGCGACAAGATCCAC ACCTTCTGGGAGATCACGCGGAGGCAGCTGGAGGAGAAGAAGGCTGAGCTGCGCAACAAAGACCGGGAGA TGGAGGAGGCCGAGGAGAGGCACCAGGTGGAGATCAAGGTATACAAGCAGAAGGTGAAGCACCTGCTGTA CGAGCACCAGAACAACTTGGCTGAGGTGAAGGCTGAGGGCACCGTGGTCATGAAGCTGGCCCAGAAGGAG CACCGCACCCAGGAGGGCGCTCTGCGCAAGGACATGCGGGTGCTGAAGGTGGAGCTCAAGGAGCAGGAGC TGGCTAACGAGGTGGTCATAAAGAACCTGTGCCTGAAACAAGCCGAGGAGATCACCAAGATGCGAAATGA CTTTGAAAGGCAAGTTCGAGAAATTGAGGCCAAGTATGATAAGAAGATGAAGATGCTTAGAGACGAACTT GACCTGCGGAGGAAGACTGAGATCCATGAGGTAGAGGAGAGGAAAAACGGCCAGATCAGCACTCTGATGC AGCGCCACGAGGAGGCCTTCACGGACATCAAGAACTACTACAACGACATCACTCTCAACAACCTGGCCCT CATCAACTCCCTCAAGGAGCAGATGGAGGACATGCGTAAGAAGGAGGAACACATGGAGAGGGAGATGGCG GAGGTGACTCTGCAGAACCGGCGGCTGGCCGACCCGCTGCAGAAAGCCAAGGACGAGATGAACGAGATGC AGAAGAGACTGGGGAACCACGAGAGGGACAAGCAGATTCTGGTCTGTACAAAGGCACGTTTGAAGGTGGC TGAGAGGGAGCTAAAGGACCTGAAATGGGAACATGAGGTCCTGGAGCAGAGGTTCATCAAGGTGCAGCAG GAGAGGGAAGAGCTCTATCGGAAGTTTGCAGACGCCATCCAGGAGGTGCAGCAGAAGACGGGCTTCAAGA ACCTGCTGCTGGAGCGGAAGCTGCAGGCCCTGAACGCTGCTGTGGAGAAGAGAGAGGTTCAGTTCAATGA GGTGCTGGCCGCCTCCAACCTGGACCCCACAGCATTAACGCTTGTGTCCCGCAAACTTGAGGATGTTCTA GAATCGAAGAATACCACCATCAAGGACTTACAGTATGAGCTGGCCCGGGTCTGCAAGGCCCACAATGACC TGCTGCGTACATATGAGGCGAAGTTACTGGCCTTCGGGATCCCCCTGGACAATGTGGGGTTCAAGCCCCT GGAGACAGCAGTGATTGGACAGACACTGGGACAGGGCCCTGCAGGACTTGTGGGTGCCCCTACATAGCCA TCTTCTCAGCCTTCAACCTTTACAGCCTTCCCCTCTGTCCAAGTAATACCTCTATGCCCAACACAGGCAA GGGTTTGTTTTCTAGACTTACCCGTCAGCAATGAAAGTTTCCCACATTTACTTTAAAAAAAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3592695 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3592695 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19566 same embryo
	EMAGE:19562 same embryo
	EMAGE:19564 same embryo
	EMAGE:19563 same embryo
	EMAGE:19565 same embryo
	EurExpress:euxassay_004857 same experiment
	MGI:4825000 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source