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EMAGE:19564

Sec61a1 Sec61 alpha 1 subunit (S. cerevisiae) ( MGI:1858417)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19564 EMAGE:19564 EMAGE:19564 EMAGE:19564 EMAGE:19564
"Pseudo-wholemount" of euxassay_004866. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004866_01 euxassay_004866_02 euxassay_004866_03 euxassay_004866_04
EMAGE:19564 EMAGE:19564 EMAGE:19564 EMAGE:19564 EMAGE:19564
euxassay_004866_05 euxassay_004866_06 euxassay_004866_07 euxassay_004866_08 euxassay_004866_09
EMAGE:19564 EMAGE:19564 EMAGE:19564 EMAGE:19564 EMAGE:19564
euxassay_004866_10 euxassay_004866_11 euxassay_004866_12 euxassay_004866_13 euxassay_004866_14
EMAGE:19564 EMAGE:19564 EMAGE:19564 EMAGE:19564 EMAGE:19564
euxassay_004866_15 euxassay_004866_16 euxassay_004866_17 euxassay_004866_18 euxassay_004866_19
EMAGE:19564 EMAGE:19564 EMAGE:19564 EMAGE:19564 EMAGE:19564
euxassay_004866_20 euxassay_004866_21 euxassay_004866_22 euxassay_004866_23 euxassay_004866_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19564Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19564_wholemount_strong.wlz
19564_wholemount_moderate.wlz
19564_wholemount_weak.wlz
19564_wholemount_possible.wlz
19564_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19564_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 23 24
vibrissa
moderate moderate
regionalmoderate expression: see section 06 07 08 20
mandible
strong strong
regionalstrong expression: see section 09 10 11 12 13 16 17 18 19 moderate expression: see section 04 05 06 07 08 20 21 22 23 24
lower jaw incisor
strong strong
regionalstrong expression: see section 12 15 16 17
maxilla
strong strong
regionalstrong expression: see section 09 10 16 17 18 19 moderate expression: see section 06 07 08 20 21 23
upper jaw incisor
strong strong
regionalstrong expression: see section 11 12 15 16
cranium
strong strong
regionalstrong expression: see section 01 02 03 04 05 21 22 23 24
clavicle
moderate moderate
regionalmoderate expression: see section 12 13 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6558
Entity Detected:Sec61a1, Sec61 alpha 1 subunit (S. cerevisiae) ( MGI:1858417)
Sequence:sense strand is shown

>T6558
CGGGGCCGGGCGTGTAGTCGGATCAGCCGCCGCCATGGCGATCAAATTTCTGGAAGTCATCAAGCCATTC
TGTGTCATCTTGCCGGAAATTCAGAAGCCAGAGAGAAAGATTCAGTTTAAGGAGAAAGTTCTGTGGACAG
CCATCACCCTCTTCATCTTCTTAGTATGCTGTCAGATCCCCCTGTTTGGCATCATGTCTTCAGACTCAGC
TGACCCGTTCTACTGGATGCGAGTGATTCTGGCCTCTAACAGAGGTACCTTAATGGAGCTGGGCATCTCT
CCTATTGTCACCTCCGGTCTTATCATGCAGCTCTTAGCTGGTGCCAAGATAATTGAAGTTGGGGACACCC
CGAAAGACCGAGCTCTCTTCAATGGAGCCCAGAAGTTGTTTGGAATGATCATTACTATCGGTCAGTCCAT
TGTGTATGTGATGACGGGCATGTACGGGGACCCTTCGGAGATGGGTGCTGGAATCTGCCTGCTGATCACC
ATTCAGCTCTTTGTTGCTGGCTTAATTGTCCTACTTTTGGATGAACTTCTGCAAAAAGGGTACGGCTTGG
GCTCTGGCATTTCTCTCTTCATTGCGACTAACATCTGTGAGACCATTGTGTGGAAGGCATTCAGCCCCAC
CACTGTCAACACTGGCCGAGGGATGGAATTCGAAGGCGCCATCATAGCGCTATTCCACCTGCTGGCCACC
CGCACAGACAAGGTCCGAGCCCTTCGAGAGGCATTCTACCGCCAGAACCTCCCCAACCTCATGAACCTGA
TTGCCACCATCTTCGTCTTTGCCGTTGTCATCTATTTCCAGGGCTTCCGAGTGGACCTTCCAATCAAGTC
GGCGCGTTACCGAGGCCAGTACAACACCTATCCCATCAAGCTCTTCTACACCTCCAACATCCCCATCATC
TTGCAGTCTGCTCTGGTGTCCAACCTGTATGTCATCTCCCAGATGCTGTCAGCACGCTTCAGTGGCAACC
TGCTGGTTAGCCTGCTGGGCACCTGGTCGGATACATCATCTGGGGGCCCAGCCCGAGCTTACCCCGTTGG
TGGCCTGTGTTACTACCTGTCTCCTCCGGAATCGTTCGGCTCAGTGCTGGAGGACCCTGTCCATGCGGTC
GTGTACATCGTGTTCATGCTGGGCTCCTGTGCATTCTTCTCCAAAACATGGATCGAAGTCTCAGGTTCTT
CTGCCAAAGATGTTGCAAAGCAGCTGAAGGAGCAGCAGATGGTGATGAGGGGCCACAGGGAGACCTCCAT
GGTGCATGAGCTCAACCGGTACATCCCCACAGCCGCAGCCTTTGGTGGGCTGTGCATTGGGGCCCTCTCT
GTCCTGGCTGACTTCCTGGGTGCTATCGGGTCTGGAACTGGGATCCTGCTTGCGGTCACCATCATCTACC
AGTACTTTGAGATCTTCGTCAAGGAACAGAGCGAGGTGGGCAGCATGGGAGCCCTTCTCTTCTGAGCCTT
GCGTGCCCGTGGACCAGGGTGAGGGCACAGGCTCCAGAACTGCCCAGGAAGGGTGCTCTTACTGCTGGAG
CATGCTACTGTGGCATAGGGACTTTAATTTTTTTTTTTAATTTTTTTTAAAATTTCATATCTTTTCATTC
CACTGTGTAAAGTGCTAGGAAATTTCCAATTTGAAATTTTGCTTTTTCTGGCATTGGCAAAAAACCAGTA
GAAGTGAATTGTTAGCTGATGGCCAGGGGGTGTGATAACAAATTGTCCTCAAGTATCCCATGTAATTTTT
ATTTAAACCCTTGCACTTCTCAGTGCCTGGCAGCTGCAAGGGTCCCAGCTGCCCCGAGGCAGTCTGTGGC
CCTGGTTGAAGGAAGCACAAACTAGATGTCTGCATCTCATCTGGGCGTGCAGACAGGTGAGGATCAGCAG
GGTCCACGTGTCTGCTGTTGCCAGCAAAGGGGGTCACTGGCTTTACCTGGAGCCTGGCCAGCTAGGTAGC
TGGACGTATATAAAGGGTCCTCGTGCTTGTTCTTTGCCAGTTCCCACTAGAAAAGGCGCTCCTGTGGTGG
CCACCTCTGTCTTCCTACTGAGAACTTCCTGTTCCAGGTTTTAGAATTTTCTTATTGTCGTTAGGACCAA
ATGTAGACTGGCTCAGGACCTGTAAGGAAATCACAGGTTCAGAGATCTCTTAGGGAAGTTGCTCAAAATC
CTGTTTGTAGTTAAGGTGTCTGGGGTAACAGATAACATGGGCCTGAGGGAGCCCTCAGGTCTATAGGAAA
GGCCCCTCTTAAGACAGAAACGGTTTAACAGCAAGGGTACAAAGGCTGTAGTTTGAGGCCAGAGCAATTA
GCGCTTGTCAGCTTACCTTACAACACAGAGGGTCCAGCTGTTCACACAAGGGGGCAGGAGCTCTGGTGGG
TGTGCAGCCATGTGGTCACAGCCTGCCAGCAGCAAGACACCTGGGTCCACCACACCTCAGTGGAAGTAGC
AGAATGCCTCCCTGACCCGGGGCTCTTAGGGACTCTTGCTGCTATGCTTGCCAGCAAATGGCAATCAAGG
GACAATTAGCCACTTGCCCCGACACAGGTCAGCATCAGGGCCAGATCCCTTGCACACAGCCCAGGGCGCA
CACCATATCACACGTGGCCTTGTCTAGACCCAGTCCTGAGCAGAGGAGCCTGGTGCCCTCCCAGTCCACA
GGGTCTTCCCACTGCCCCTCCTCTGCCACTGCCACAGAGCAGCTTCCCACCTACCCTAGGGACTCCACAG
CTTCTGGCAAGCAGGTGCAGAGTCCCCAGCTCTGGGCTGTGCTGGGGAGTGTCAGGCCTGGAGTCCACAG
CCTCACCTGCTGCTGTAGCTGTCAGCTGGACCTGGTGGCTTGTGGTTGATGGTCACTATTAGTGGGTGAG
TGGGGATGACCTGGCTTCCAAGAGGACTGTCACTGTGGACACCAAAATGGCTTAACTGAGATACAGTGAA
AACATGACAAATAAACCAACTTTTTACAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3592772 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3592772 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19566 same embryo
 EMAGE:19561 same embryo
 EMAGE:19562 same embryo
 EMAGE:19563 same embryo
 EMAGE:19565 same embryo
 EurExpress:euxassay_004866 same experiment
 MGI:4827937 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS