EMAGE mouse gene expression spatial database

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EMAGE:19585

Vil1 villin 1 ( MGI:98930)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005091. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005091_01	euxassay_005091_02	euxassay_005091_03	euxassay_005091_04

euxassay_005091_05	euxassay_005091_06	euxassay_005091_07	euxassay_005091_08	euxassay_005091_09

euxassay_005091_10	euxassay_005091_11	euxassay_005091_12	euxassay_005091_13	euxassay_005091_14

euxassay_005091_15	euxassay_005091_16	euxassay_005091_17	euxassay_005091_18	euxassay_005091_19

euxassay_005091_20	euxassay_005091_21	euxassay_005091_22	euxassay_005091_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19585_wholemount_strong.wlz

19585_wholemount_moderate.wlz

19585_wholemount_weak.wlz

19585_wholemount_possible.wlz

19585_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19585_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

stomach

strong

regional

strong expression: see section 08

hindgut

strong

regional

strong expression: see section 11 12 13 14 15 16

rectum

strong

regional

strong expression: see section 11 12

midgut

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 moderate expression: see section 18 19

kidney calyx

moderate

regional

moderate expression: see section 06 07 08 09 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7482
Entity Detected:	Vil1, villin 1 ( MGI:98930)
Sequence:	sense strand is shown >T7482 CCCAGGTGGTGGCTGCCTCTTCCAGACAGGCTCGTCCACCATGACTAAACTGAATGCCCAAGTCAAAGGC TCTCTCAACATCACCACTCCCGGGATACAGATATGGAGGATCGAGGCTATGCAGATGGTACCTGTTCCTT CCAGCACCTTTGGAAGCTTCTTCGATGGTGACTGCTATGTAGTCCTGGCTATCCACAAGACCAGCAGCAC TCTCTCCTATGATATCCACTACTGGATTGGCCAGGACTCGTCCCAGGATGAGCAGGGGGCAGCTGCCATC TACACCACACAGATGGATGACTACCTGAAGGGCCGGGCTGTCCAGCACCGCGAGGTTCAAGGCAACGAGA GCGAGACTTTCCGGAGCTACTTCAAGCAAGGCCTTGTGATCCGGAAAGGGGGAGTGGCTTCCGGCATGAA GCACGTAGAAACAAACTCCTGTGATGTCCAGCGACTGTTGCACGTCAAGGGCAAGAGGAATGTGCTGGCT GGAGAGGTGGAAATGTCCTGGAAGAGTTTCAACAGAGGGGATGTCTTCCTGCTGGACCTTGGGAAGCTTA TTATCCAGTGGAATGGGCCAGAGAGTAACCGCATGGAGAGACTTCGGGGCATGGCCTTGGCCAAAGAGAT CCGAGACCAGGAACGGGGTGGACGTACCTACGTAGGTGTGGTGGACGGGGAGAAGGAAGGGGACTCCCCA CAGCTGATGGCAATTATGAACCACGTGCTGGGCCCACGCAAAGAACTGAAGGCTGCTATTTCTGACTCAG TGGTGGAGCCGGCCGCTAAGGCTGCACTCAAGCTGTACCATGTGTCTGACTCCGAAGGAAAACTGGTGGT TAGAGAAGTTGCTACTCGGCCACTCACACAAGACCTGCTCAAGCATGAGGACTGTTACATCCTGGACCAG GGAGGCCTGAAGATCTTTGTGTGGAAGGGAAAAAATGCCAACGCTCAGGAGAGGAGCGGAGCCATGAGCC AGGCCTTGAACTTCATCAAAGCCAAGCAGTACCCACCGAGCACGCAGGTTGAGGTGCAGAATGACGGGGC CGAGTCCCCCATCTTCCAACAACTCTTCCAGAAGTGGACAGTGCCCAACCGGACCTCAGGCCTCGGCAAA ACCCACACTGTGGGCTCTGTGGCTAAGGTGGAACAGGTGAAGTTTGATGCTCTGACCATGCATGTACAAC CTCAGGTGGCTGCCCAGCAGAAAATGGTGGATGACGGGAGTGGGGAAGTGCAGGTGTGGCGCATCGAGGA CTTGGAGCTGGTGCCTGTGGAGTCCAAGTGGCTGGGCCATTTCTACGGTGGTGACTGCTACCTGCTGCTC TACACCTACCTCATAGGGGAAAAGCAACACTACTTGTTATATATCTGGCAGGGCAGCCAGGCCAGCCAGG ATGAAATTGCAGCCTCGGCGTATCAAGCCGTCCTGTTGGACCAGAAGTACAATGACGAGCCAGTACAGAT CCGGGTCACGATGGGCAAGGAGCCGCCTCACCTCATGTCTATCTTCAAGGGCCGCATGGTGGTTTATCAG GGAGGCACCTCCCGAAAGAACAACTTGGAGCCTGTGCCCTCTACGAGGCTATTTCAGGTCCGAGGGACCA ATGCTGATAACACCAAGGCTTTTGAGGTGACAGCCCGGGCCACGTCCCTCAACTCCAATGATGTCTTCAT ACTCAAGACTCCGTCCTGCTGCTACCTGTGGTGTGGGAAGGGCTGCAGTGGGGATGAGAGGGAGATGGCC AAGATGGTTGCTGATACCATCTCTCGGACGGAGAAACAAGTGGTAGTAGAGGGGCAGGAGCCAGCCAACT TCTGGATGGCTCTGGGCGGGAAGGCGCCCTACGCCAACACCAAGAGGCTGCAGGAGGAAAACCAAGTCAT CACTCCTCGGCTCTTCGAGTGCTCCAACCAGACCGGACGCTTTCTGGCCACAGAGATCTTTGACTTCAAT CAGGATGACCTGGAGGAGGAGGATGTGTTCCTATTGGATGTCTGGGACCAGGTCTTCTTCTGGATAGGGA AACATGCCAATGAGGAAGAGAAGAAGGCTGCAGCTACAACTGTACAAGAATACCTCAAGACCCACCCTGG AAACCGAGACCTTGAGACCCCTATCATCGTGGTGAAGCAGGGACACGAGCCCCCCACCTTCACAGGCTGG TTCCTGGCTTGGGATCCCTTCAAGTGGAGTAACACCAAATCCTATGATGACCTTAAGGCAGAGCTGGGAA ACTCTGGGGACTGGAGCCAGATTGCTGACGAGGTTATGAGCCCGAAAGTGGACGTTTTCACTGCCAATAC CAGTCTGAGTTCTGGGCCCCTGCCCACCTTCCCCCTGGAGCAGCTGGTAAACAAGTCTGTAGAGGATCTC CCTGAGGGTGTGGACCCCAGCAGGAAGGAGGAGCACCTGTCCACCGAAGACTTCACTAGGGCCTTGGGCA TGACTCCAGCTGCCTTCTCTGCCCTGCCTCGATGGAAGCAACAAAACATCAAGAAAGAAAAAGGACTGTT TTGAGAATTGAAGCTCTCTGGCTGTCCAGCAGCCCCTACCCTGCCTTCAAGGGCTTTGTGCCGCCATTAC TGGTTTTAGTCCTGTGGCAGATGAAAATGTCCAATTGTACCTGTGAGCCACAGTGTGACAATTCCTTTTG TTTATAATAGTAATTTGCCCATTCCTTCAGACGCATGCCACAGACCCATGGAAATCTTGTAGAGTTTTCT TTTCTTAGATGGACAGCTAAGTACTCCAGGAGACATTAGCGTCTGGGGGTTTCTCTGGCACCGTCACTCA CTCAGGATCTTATCCTGATCTTACCCTCCTCACACTCAAAAGGGAGGGCTAAGGCCAAAGCTGGGCTTAC AGCTCTAACCCAGAGCCTTTGCAAAGCTCCACAGACTCCTCAAATGACAACACCAGGAACATGGGTTTGC TACGTGAAGTCCAATCAGAAGCCAATAGGTGATTTTCTCTTAAAACTGGTTATCCAGTGTCCCCAGGAAC ATGTCCCTTTAAACAAATAAATCAAACTAATATGAGGTTAATAAAGGCTTTAATGTCTCTCACACATTAA AACAAAACAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4236751 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4236751 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19586 same embryo
	EMAGE:19587 same embryo
	EMAGE:19588 same embryo
	EMAGE:19584 same embryo
	EurExpress:euxassay_005091 same experiment
	MGI:4829159 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source