EMAGE mouse gene expression spatial database

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EMAGE:19588

Paqr7 progestin and adipoQ receptor family member VII ( MGI:1919154)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006273. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006273_01	euxassay_006273_02	euxassay_006273_03	euxassay_006273_04

euxassay_006273_05	euxassay_006273_06	euxassay_006273_07	euxassay_006273_08	euxassay_006273_09

euxassay_006273_10	euxassay_006273_11	euxassay_006273_12	euxassay_006273_13	euxassay_006273_14

euxassay_006273_15	euxassay_006273_16	euxassay_006273_17	euxassay_006273_18	euxassay_006273_19

euxassay_006273_20	euxassay_006273_21	euxassay_006273_22	euxassay_006273_23	euxassay_006273_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19588_wholemount_strong.wlz

19588_wholemount_moderate.wlz

19588_wholemount_weak.wlz

19588_wholemount_possible.wlz

19588_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19588_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

strong

regional

strong expression: see section 13 moderate expression: see section 11 12

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 13 moderate expression: see section 11 12

telencephalon ventricular layer

moderate

regional

moderate expression: see section 03 04 18 weak expression: see section 05 06 07 19 20 21 22

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 13 moderate expression: see section 11 12 14

pons ventricular layer

strong

regional

strong expression: see section 13 moderate expression: see section 08 09 10 11 12 14 15 16 17 weak expression: see section 07

midbrain ventricular layer

strong

regional

strong expression: see section 13 moderate expression: see section 08 09 10 11 12 14 15 16

spinal cord ventricular layer

strong

regional

strong expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7532
Entity Detected:	Paqr7, progestin and adipoQ receptor family member VII ( MGI:1919154)
Sequence:	sense strand is shown >T7532 CACAGTTTTCCTGACTTCTCTTCCGGGCCCTCCGGCATGGCGATGGCAGTAGCCCAGAAGTTCAACCACC TTCTGTCCAGCCTGTGGCACGTGGGCCAGAAGCCTCCGCAACCAGAACCTGTGTTTACAGTGGACCGGGC CCAGGTGCCGCCACTTTTCTGGAAGCCGTACATCTATGCTGGCTACCGGCCGCTGCATCAGAACTGGTGT TTCTACTTCCGCACACTGTTTCAGCGGCACAACGAGGCTGTGAACGTGTGGACCCACCTCCTGGCGGCCC TGGCTCTGCTGCTGCGGCTGATCGGCTTGGCGGCAAGTGTGGACTTCCGGGAAGACCCTCACGCGCTGCC CCTCTTCTTCATCGTCTTGGCCTCCTTCACCTACCTCTCGTTCAGTGCTGTGGCTCACCTCCTGCAGGCC AAGTCGGAGTTCTGGCATTACAGCTTCTTCTTCTTGGACTATGTGGGTGTGGCCGTGTACCAGTTTGGCA GTGCCCTGGCACACTTCTACTATGCCATAGAGCCGTCCTGGCATGACAAGGTGCAGGCTATTTTCCTGCC CACGGCCGCCTTCCTGGCCTGGCTTTCCTGCGCTGGCTCCTGCTACAACAAGTACAGTCAGAAGCCGGGT CTGCTGGGGCGCATTTTCCAGGAGGCGCCATCAGCGCTGGCCTATGTGCTGGACATCAGCCCCGTGTTGC ACCGCATCATAGTGTCTCCCCTCCCTGCCGAGGAGGATCCCGCTCTTCTCTATCACAAATGCCAAGTGGT TTTTTTCCTTCTAGCTGCTGCCTTTTTCTCCACGGTTATGCCTGAGAGTTGGTTCCCCGGCAGCTGTCAC ATCTTTGGGCAGGGACACCAAGTTTTCCATGTCTTTTTGGTGCTGTGCACTCTGGCCCAGCTAGAGGCGG TGACACTGGATTATCAGGCCCGGAGGGGCATATACGAGCCTCTGCACGCCCGTTGGCCCCACAACTTCTC TGGCCTCTTCCTGCTCACCGTGGCCAGCAGCAGCCTCACCGCGCTCCTCCTCAGCCAGCTGGTGCGGCGC AAACTCCATCAGAAGACCAAGTGAAAGCGGGTGGGTGGGAGCTGGCAGGGTGAGAGAAGGTGCTAGGGAC AAAAGCCTGGACTTGGCTCCAGATCGGAACAAGGCCTGGTAAAGCTGGTTCTATCTGGCAGGCCATGACT CCCTGCATACAAGCTCAATGGCCAAAGTGATGCTGCTAGCCAATCCTTGGGCCTGCAGATTGGCTGGGAG CTGTAGAGCCTTGCTCAGTTCCCTGGGAGGGGCAGAGATGAGGGACTCCTGACTGCCCCTCCCTTGCCGC CTATCAGGGCTAAGGGTTGGAGTTCAGCTTTGGTCACCACACTGGTTTGGGGTTTTCAGGTTGCTGGGCG AGAAGACTCAGGCCAGTGTCAAATTTGAGCAGAGAGGGAGTGGAAGTCTTAGCAGCCACCACCTACCAGA GCTCACTGGTGGAGGGAAAAGAAACAGGCCCAAAGATGGGTAGTGTTTTAACTTCACTATCCCCTGCACC ACAGCCTGGATCGCTGGGTTCCAGAGAGTCCCCAAAGATAAGAGATCCCCTCTCCTGCCCACATCCCTGC ATCACAGCCCAGCCAGAAAACCTCACAGCTAGCATTGGCCTGTATCTCTTGCACACTGTTTCCAGCTTCT CCCCACAACTCATTTATGATTTTGAGATATGTCTCCTTAGCTCACTGTTTCCTGGGGCTCCAGGCTTTAG AACCGTTAGGAATTGTCAAGGAAAGCTCAAAGTGTTGACGAGTCAGCACTGCAGGGCACTCAGAGCCTTG GCTTAGGAAGGGTGAGGAGGAGTTTGCAGGCTGAGCTTAGAAGGGCGTTGCAGGCAAAAGGGAAGCCTGT GCCGAGATTCGAGATGCAGAGATGCAGGGAGTTCAGGGGGGGGGCTAAAGGCAAGTTTGGTCCCTGGGGC TGAGAGGCAAGACTGGTGGTCCCTGATATGCCCAAGATTCAATAAAATGACTATGATATTGCTAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4239768 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4239768 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19586 same embryo
	EMAGE:19585 same embryo
	EMAGE:19587 same embryo
	EMAGE:19584 same embryo
	EurExpress:euxassay_006273 same experiment
	MGI:4827047 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source