EMAGE mouse gene expression spatial database

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EMAGE:19592

Slc12a1 solute carrier family 12, member 1 ( MGI:103150)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005056. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005056_01	euxassay_005056_02	euxassay_005056_03	euxassay_005056_04

euxassay_005056_05	euxassay_005056_06	euxassay_005056_07	euxassay_005056_08	euxassay_005056_09

euxassay_005056_10	euxassay_005056_11	euxassay_005056_12	euxassay_005056_13	euxassay_005056_14

euxassay_005056_15	euxassay_005056_16	euxassay_005056_17	euxassay_005056_18	euxassay_005056_19

euxassay_005056_20	euxassay_005056_21	euxassay_005056_22	euxassay_005056_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19592_wholemount_strong.wlz

19592_wholemount_moderate.wlz

19592_wholemount_weak.wlz

19592_wholemount_possible.wlz

19592_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19592_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7464
Entity Detected:	Slc12a1, solute carrier family 12, member 1 ( MGI:103150)
Sequence:	sense strand is shown >T7464 CTGGATGGGGCGCAGCCTTGAGGACCATTCTGAAGTTTGCATGGGAGACCACATATCAACAATACATCAG GGATCTAGCTATCGGGTCATCTAAAACCGAAGCCAAGCGCTCCGTATTATAACCACACTATTGTCTATTT TAAAGCAGAAATACGTAGATTAAAACAACAACCACTATAAAATAGGTAGTTCCACAGTAGATTTTGGAAG ATGTCGGTCAGCATCCCTTCCAACTCAGTGCCCAGTAGTGCCAGTCGCTTTCAGGTCCATGTTATAAACG AGGGCCATGGCAGTGCGGCAGCCGTGGGCGACAGTGCAGACCCCCCACATTATGAAGAGACCTCTTTCGG GGACGAAGCCCAAAACAGACTGAGAATCAGCTTTAGGCCTGGGAATCAGGAGTGCTATGACAATTTTCTC CAAACTGGAGAGACTGCCAAAACAGACACCACGTTTCACGCCTACGACTCTCACACAAACACATACTACC TACAAACCTTTGGCCACAACACCATGGACGCCGTCCCCAAGATTGAATACTACCGCAACACAGGCAGCGT GAGCGGGCCCAAGGTCAACCGGCCCAGCCTGCTGGAGATCCACGAGCAGCTGGCTAAGAATGTGACTGTG GCCCCTGGTTCAGCTGACAGGGTTGCTAATGGAGATGGGATGCCTGGAGATGAGCAAGCAGAAAACAAGG AAGAAGACATGACTGGAGTTGTGAAGTTTGGATGGGTGAAAGGTGTGCTGGTGCGATGCATGCTGAACAT CTGGGGAGTCATGCTCTTCATTCGCCTCTCCTGGATTGTAGGAGAAGCGGGAATTGGTCTTGGAGTTCTT ATAATTCTTCTTTCCACCATGGTAACCTCTATCACTGGGTTGTCAACTTCTGCAATAGCAACAAACGGGT TTGTTCGTGGAGGTGGAGCCTACTACCTGATTTCCCGGAGCTTAGGGCCTGAGTTCGGTGGGTCAATAGG CTTGATCTTTGCTTTTGCCAATGCAGTGGCTGTCGCTATGTATGTGGTGGGATTCGCGGAGACTGTCGTG GATCTTCTTAAGGAGAGCGATTCAATGATGGTGGATCCAACCAATGACATCCGCATCATCGGTTCCATCA CCGTGGTGATTCTTCTAGGAATCTCAGTAGCTGGGATGGAGTGGGAAGCAAAGGCTCAAGTCATTCTCCT GGTCATTCTTCTCATTGCCATTGCAAACTTCTTCATTGGAACTGTCATCCCATCCAACAATGAGAAGAAA TCCAGAGGCTTCTTTAACTACCAAGCATCTATATTTGCGGAAAACTTTGGGCCAAGCTTCACAAAGGGGG AAGGCTTCTTCTCGGTCTTTGCCATCTTTTTCCCAGCTGCTACTGGGATTCTTGCTGGTGCCAACATCTC TGGAGACCTGGAGGATCCCCAGGACGCCATCCCCAGAGGGACCATGCTGGCCATCTTCATCACCACCGTG GCCTACATAGGTGTTGCTATTTGCGTAGCCGCCTGTGTGGTCCGAGATGCCACTGGCAGCATGAATGACA CCATCGTTTCTGGGATGAATTGCAATGGCTCTGCGGCCTGTGGGTTGGGCTACGACTTCTCAAGATGTCA ACACGAGCCATGTCAGTATGGGCTGATGAACAATTTCCAGGTCATGAGCATGGTGTCCGGGTTCGGGCCC CTCATCACTGCAGGGATCTTTTCGGCAACACTGTCTTCCGCCCTGGCCTCCCTCGTCAGTGCGCCCAAAG TATTCCAGGCTCTGTGCAAGGACAACATCTTCAAAGGACTGCAGTTCTTCGCAAAGGGCTATGGGAAAAA CAACGAGCCCCTGAGAGGGTACTTCCTCACTTTTGTGATAGCCATGGCATTCATTCTCATCGCCGAGCTG AATGTCATTGCCCCCATCATCTCCAACTTCTTCCTGGCCTCATATGCGCTTATTAATTTCTCCTGTTTCC ATGCCTCGTATGCCAAATCTCCAGGATGGAGGCCTGCATATGGGATTTACAACATGTGGGTATCTCTTTT TGGAGCGATTTTGTGCTGTGCAGTCATGTTTGTCATCAACTGGTGGGCAGCTGTCATCACTTATGTCATT GAGTTATTCCTCTATATCTATGTGACTTATAAGAAGCCAGATGTGAACTGGGGCTCCTCCACACAGGCTC TGTCCTATGTGAGTGCTTTAGACAACGCTCTGGAATTAACCACAGTGGAAGACCATGTGAAAAACTTCAG ACCCCAGTGCATTGTCTTAACAGGCGGACCCATGACTAGACCCGCTCTCTTGGATATAACCCACGCCTTT ACGAAGAATAGTGGCCTCTGCATCTGCTGCGAAGTCTTCGTGGGACCTCGCAAGCTGTGTGTTAAAGAGA TGAACAGCGGCATGGCTAAAAAACAGGCCTGGCTTATAAAGAACAAAATCAAAGCTTTTTATGCTGCAGT GGCTGCAGACTGCTTCAGGGACGGTGTCCGGAGCCTCCTTCAGGCCTCAGGCTTGGGGAGAATGAAGCCA AACACTCTCGTGATTGGATACAAAAAGAACTGGAGGAAAGCTCCCTTGTCAGAGCTGGAGAACTACGTGG GAATCATACATGACGCCTTTGACTTTGAGATTGGCGTGGTCATAGTCAGAATCAGCCAAGGCTTTGACAT CTCTCCGGTTCTCCAGGTACAAGACGAACTGGAGAAACTGGAACAGGAGAGATTGGCTCTGGAGGCCGCA ATCAAAGACAACGAGTGTGAGGAGGGCAAGGGGGGCATCCGAGGCTTGTTTAAGAAGGCCGGCAAGCTGA ACATTACCAAGCCAGCCCCCAAGAAAGACGGCAACATCAGCAGCATCCAGTCAATGCACGTCGGGGAGTT CAATCAGAAACTGGTGGAAGCCAGCGCTCAGTTTAAAAAGAAGCAGGGAAAAGGCACGATTGATGTTTGG TGGTTATTTGATGATGGAGGGTTAACACTGCTTATCCCATATATCTTGACTCTCAGAAAAAAATGGAAAG ATTGTAAGTTAAGAATCTATGTTGGAGGAAAGATCAACCGCATCGAAGAGGAAAAAATTTCAATGGCTTC ACTTCTCAGCAAATTTAGAATAAAATTTGCAGACATCCATATAATTGGAGACATCAACATTAAGCCAAAC AAAGAGAGCTGGAAAGTCTTTGAAGAGATGATTGAACCTTATCGGCTTCATGAAAGCCACAAAGATTTGA CCACTGCTGAGAAATTAAAAAGAGAAAGTCCATGGAAAATTACAGATGCAGAACTGGAAGCAGTCAAGGA AAAGAGTTACCGTCAAGTTCGACTGAACGAGCTCTTACAGGAACACTCCAGAGCCGCCAACCTCATCGTG TTGAGCCTTCCAGTGGCAAGAAAAGGATCTATTTCGGATTTGTTGTACATGGCGTGGTTAGAAATCCTGA CCAAGAACCTTCCTCCTGTGTTACTGGTTAGAGGAAATCACAAAAATGTCTTAACATTTTACTCTTAAAG CCTGAAGGATGAGAAATGTTGTGCCTTGGTGTCTGAATAATTCTGAAACACATCTGGTTCTTTGTGTTTT AAAACCTCTTCTATGAATATACAAACCTCTGGAGACTAACCTAACCGATTCTACATAAGTTTTGTCTTTT AAAAAAAAGTTATTATTAATGGGAGTTTTTTTTCCCACTAGCTTAAAAAACATCGAAAGCCATGAGACTC CTAGAAAAACACTTTTCCGTTGTTGCTGGTAAATAAGAAATTAATAAAAGCTGGCTGACATGCTTACAGG ATATGCTAATCTAGCTGTGAGCTCCAGGCAAACTTTATCTCGTTTCTGTAGAGTCTGCCGCATGGCCCTG GTGCAGGCCACAGGCCCACAGCGGGCTCTCCTTAAGTTCTTATTGACAGGCTGGTATGGTGAAGGCAGGT GGCTATCATAGGAAAAGGCCTGCTGGGTCAGTGGCCTGATTCTGCTTGGCCTCCTGAGAAGTTTGTGAAG ATTTAAAAATCAATATCTGGAAACTCTAAGAAGGACTGAAGTCTACAAAGTGTTCAAAGTAGTTACCTAA ATAAGGTTTATTTAATGCAGCGGATAATAAGCTTACCCTCTGATCACATGTAGCTTACACTTGCTGCTAG CTGGAGGTATATTAGGTAGGTGAGCAAGAGACCCATGTTCCACTTACATGAAATAGAGTCTTAACATACA AATATCTATTGTCTTCCTGGCTAAATATTTTGGTATTTCTACCTTAGTACTACTTTATTTTTTTCTCTCT CTAAGTGGTATGCATAGATGAAATAAAAATCGATGATCTCATAGAAAATTAATTTCACAGGTTTCCAATC CACTATTAAAAATGTATCCAGATCCTACATTTATTTACTTTCTAAATATATAGGTATTTAACCCGTGCTT TACAGAATAACAAAGATATAAATGGAGACACATGTAAAATTTAAAAATAGAGGTGCTGATTTTTCTTCTT GTTAGTGTGTGTATAATATTTAATCACAGAAAAAATGCTTATATGATAAATGGCTTGCTTTTGGTTTGTG TCATTTCTTGGCATATTTTGACTGGGGGATGTTCGTGGGGGCAAATTGACCTGAAATAAAATCACTATAT TTTGTTTGCTTTGGCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4236020 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4236020 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19590 same embryo
	EMAGE:19589 same embryo
	EMAGE:19591 same embryo
	EurExpress:euxassay_005056 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source