EMAGE mouse gene expression spatial database

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EMAGE:19593

Ralgps2 Ral GEF with PH domain and SH3 binding motif 2 ( MGI:1925505)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004996. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004996_01	euxassay_004996_02	euxassay_004996_03	euxassay_004996_04

euxassay_004996_05	euxassay_004996_06	euxassay_004996_07	euxassay_004996_08	euxassay_004996_09

euxassay_004996_10	euxassay_004996_11	euxassay_004996_12	euxassay_004996_13	euxassay_004996_14

euxassay_004996_15	euxassay_004996_16	euxassay_004996_17	euxassay_004996_18	euxassay_004996_19

euxassay_004996_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19593_wholemount_strong.wlz

19593_wholemount_moderate.wlz

19593_wholemount_weak.wlz

19593_wholemount_possible.wlz

19593_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19593_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 17 18 19 20

upper leg muscle

strong

regional

strong expression: see section 01 02 03 04 05 16 17 18 19 20

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 14 15 16 17 18 19

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04 18 19 20

hand

strong

regional

strong expression: see section 01 03 04 18 19 20

foot

strong

regional

strong expression: see section 03 04 05 17 18 19

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 20

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

thymus primordium

strong

regional

strong expression: see section 09 10 11 12 13

submandibular gland primordium

strong

regional

strong expression: see section 06 07 15 16 17

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

vibrissa

strong

regional

strong expression: see section 02 03 04 05 06 07 08 18 19 20

facial vii ganglion

strong

regional

strong expression: see section 04

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 16

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 15 16 17 18 19

vagus x ganglion

strong

regional

strong expression: see section 06 15

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 04 05 06 07 15 16

spinal cord

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13

dorsal root ganglion

strong

regional

strong expression: see section 06 07 10 11 12 13 14 15

cornea epithelium

strong

regional

strong expression: see section 03 20

eye skeletal muscle

strong

regional

strong expression: see section 05 06 19

retina

strong

regional

strong expression: see section 01 02 03 20

tongue muscle

strong

regional

strong expression: see section 09 10 11 12 13

stomach

strong

regional

strong expression: see section 01 02 03 04 05

lower jaw incisor

strong

regional

strong expression: see section 10 11

lower jaw molar

strong

regional

strong expression: see section 06 17 18

upper jaw incisor

strong

regional

strong expression: see section 10 11

upper jaw molar

strong

regional

strong expression: see section 06 17 18

bladder

strong

regional

strong expression: see section 09 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8054
Entity Detected:	Ralgps2, Ral GEF with PH domain and SH3 binding motif 2 ( MGI:1925505)
Sequence:	sense strand is shown >T8054 AAGAGGTAATCCCGGAGCCGCGGGCGAAGCGGAGGCGACGGCCGCGGCTGCACAGGAATGATGCATTTGT GGCCTTGGACATGAAGTAATCAGACCTCTGTTGCTGTTACTGTAGGCTCAGGGGCTGATGAGGAAAGCAT GGACCTAATGAACGGGCAGGCAAGCAGTGTTACTATCGCAGCCACTGTTTCCGAGAAGAGTAGCAGCTCT GAGTCACTAAGCGAGAAGGGCTCTGAATTGAAGAAAAGCTTTGATGCTGTGGTGTTTGATGTTCTTAAGG TTACGCCAGAAGAATACGCGGGTCAGATAACACTAATGGATGTTCCAGTGTTTAAAGCTATTCAGCCAGA TGAACTTTCAAGTTGTGGATGGAATAAAAAAGAAAAATATAGTTCTGCACCAAATGCAGTTGCTTTCACA AGAAGATTTAATCACGTAAGCTTTTGGGTTGTAAGAGAGATTCTCCATGCTCAAACACTGAAAATAAGAG CAGAAGTTTTGAGCCACTATATTAAGACTGCTAAGAAACTATATGAACTTAACAACCTTCACGCACTTAT GGCTGTGGTTTCTGGCTTACAGAGTGCGCCGATTTTCCGGTTGACTAAGACGTGGGCGTTATTAAGTCGA AAAGACAAAACTACCTTTGAAAAACTAGAATATGTAATGAGTAAAGAAGATAACTACAAAAGACTCAGAG ACTATATAAGCAGCTTAAAGATGACTCCTTGCATTCCCTATTTAGGCATCTATTTGTCTGACTTGACCTA CATTGACTCCGCGTACCCATCCACCGGCAGCATTCTAGAAAATGAGCAAAGATCAAATCTGATGAACAAC ATTCTTCGAATAATTTCTGATTTGCAGCAGTCCTGTGAATATGATATTCCCATATTGCCTCATGTCCAAA AATACCTGAACTCTGTTCAGTATATAGAAGAACTACAAAAGTTTGTGGAAGACGATAATTACAAGCTCTC CTTAAAGATAGAACCAGGAGCAAGTACTCCACGCTCGGCTGCCTCCAGGGGGGACCTAGCAGGTCCTGAC ATAGGCGCCTCACCCCAGGGAGGGAGGAAGAGTAGTGCTGCTGCTGCTGCCGCCGCGGCTGCCGAGGGAG CCTTACTGCCACAGACGCCACCTTCCCCTCGGAACCTCATTCCACACGGACACAGGAAGTGCCACAGCCT GGGTTACAATTTCATTCATAAGATGAACACAGCAGAGTTTAAGAGCGCAACGTTCCCAAACGCAGGGCCA CGGCACCTGCTGGATGATAGTGTCATGGAGCCGCACGCACCGTCGCGAGGCCAGGCTGAGAGCTCTACAC TTTCCAGTGGAATATCCATAGGGAGCAGTGATGGTTCTGAACTAAGCGAAGAGACCTCATGGCCGGCTTT TGAAAGGAACAGATTATACCATTCTCTCGGCCCGGTGACAAGAGTGCCGCGAAATGGCTATCGAAGCCAC ACGAAGGCCAGCAGCTCTGCGGAGTCGGAAGATTTGGCGGTGCATCTGTACCCCGGAGCTGTTACTATTC AAGGTGTGCTCCGGAGGAAAACCTTGCTAAAAGAAGGCAAGAAACCCACAGTAGCATCTTGGACAAAGTA TTGGGCAGCCTTGTGTGGAACACAGCTTTTTTACTATGCCGCCAAATCTCTGAAGGCTACAGAAAGAAAG CATTTCAAATCAACGTCAAATAAGAATGTGTCTGTGGTGGGCTGGATGGTGATGATGGCTGACGACCCAG AGCATCCAGACCTCTTTCTGCTGACTGACTCCGAGAAAGGAAATTCATACAAGTTTCAAGCTGGCAGCAG GATGAATGCAATGCTGTGGTTTAAACACTTGAGCGCGGCCTGCCAGAGTAACAAGCAACAGGTTCCTACA AACTTGATGACTTTTGAGTAAACGCCTGAGACAGAAGAGGAGTGCTATTGTTCCCGTGTGGAACCGGGAC CTGCCGAGAGCCCCAGCCGCACCATCCCGTGCCAGGAAGAGCCCCCAGGCTCCAGCCCAGCCTCCGGAGC TCAGAACCAAAAGCACTAATTTCAGGGAATGAAGTGAGATGTTCCCAGAGAAAAACGATGGAGTTGCAAA GCAAACTGCCATGAACTTCGCTTCTTCTCTCCGAACTGACCTGTGGAAGCCACTGCCTTAACAGAGTGCG AGGAGAGCCGACACCAAATAGTGTGTGTGCGTCTTCTGGCGGTGCTGTGCTTGGAATAAATTGTAGCTAA TTTGCATTTCTTTTAACTTTGTACTAATTTTGGAGGGGAAACCATCTTTTTTAGATACACTTTAAAATGG AAAAACATTTCTTATGGATTCCATCAGGAGCTGTTTTAGGTGCAGTGAGAAATACCCTGCAGGTTCTGCT CAGCCTCTGCCAGTGGCTGCCTCACGTTCCCACAGCAAGACCTGGGAGGGACCTGCTGTGGAAGCTGTGG GTGCAGGCTGGGATAGATAGGGAGGGCATGCCTCGCTTTGGATGAGGGATCCATTTAAACACTACCGTTT GGAATTGAGGGCTAGAAGGAGAACATGATTTCCTGCAGGAGGGACAAAGGCTGAGTGTTCACGTGCCAGC AGGCTCCAGAACACACCAGGGTTAAAGTTTAACTTTCTGTATGAATTCATTGTTGGGCCCAAATCCGCAT AGGAGCACGGTGATCCCGCGCTCGGGTTTCTGTAGCCTTTTCACAAGTCAGGTGGGTTATCCAGCCACGG GTGTCCCAGGGAAGACCCTGGTGTTTCTTGTGCTGTTGCCCCTTGCAGTGTTTAATGTGCAGTATGCCAG ACTTATTTTTTATTGAATTTGTTTTTGTTCAGTATGAGGTTCAAAAGCATACTTGTTTAAAAGGTTCTTC ATGTATATGTATGTATTTTATTGTAAGACAAAGCAATTTTAAGAAGAATAAAGGCAAGGTTTGCAGTTCT AGGAATTGAAAATATAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30051209 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30051209 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19596 same embryo
	EMAGE:19597 same embryo
	EMAGE:19595 same embryo
	EMAGE:19594 same embryo
	EurExpress:euxassay_004996 same experiment
	MGI:4827599 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source