EMAGE mouse gene expression spatial database

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EMAGE:19596

Hand1 heart and neural crest derivatives expressed transcript 1 ( MGI:103577)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004960. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004960_01	euxassay_004960_02	euxassay_004960_03	euxassay_004960_04

euxassay_004960_05	euxassay_004960_06	euxassay_004960_07	euxassay_004960_08	euxassay_004960_09

euxassay_004960_10	euxassay_004960_11	euxassay_004960_12	euxassay_004960_13	euxassay_004960_14

euxassay_004960_15	euxassay_004960_16	euxassay_004960_17	euxassay_004960_18	euxassay_004960_19

euxassay_004960_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19596_wholemount_strong.wlz

19596_wholemount_moderate.wlz

19596_wholemount_weak.wlz

19596_wholemount_possible.wlz

19596_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19596_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 weak expression: see section 14

cervical ganglion

moderate

regional

moderate expression: see section 08 15 weak expression: see section 07

thoracic ganglion

moderate

regional

moderate expression: see section 09 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8010
Entity Detected:	Hand1, heart and neural crest derivatives expressed transcript 1 ( MGI:103577)
Sequence:	sense strand is shown >T8010 GGTCTTTGGACGTCTGAACCCTTCTCCCTCGTTGCCTACAGAAACCTTCAAGAGGTCGGCAGGTCCTTCG TATCATCTCCAGCCTTGCTCGCTCAGGACTCTCCAAACCCCAGCCAGTTCGCTGCCTCTGGAGCCCACGG TGGCCCCCTTCTGTGGGGCGCATGGGGCGGTGCTAATTGGGCTGACAGCACAGGCCAGGAGCCTCTCCAA CATGAACCTCGTGGGCAGCTACGCACATCATCACCATCATCACCACTCACACCCGACGCACCCCATGCTC CACGAACCCTTCCTGTTTGGCCCGGCCTCGCGTTGCCACCAGGAGCGGCCTTACTTCCAGAGCTGGCTGC TGAGCCCGGCTGATGCTGCCCCAGATTTCCCTGCCGGCGGGCCACCACCTACCACCGCAGTAGCAGCGGC TGCCTATGGTCCCGATGCCAGGCCGAGTCAGAGCCCAGGTCGGCTGGAGGCTCTTGGAAGCCGCCTGCCC AAACGAAAAGGCTCAGGACCCAAGAAGGAGAGGAGACGCACAGAGAGCATTAACAGCGCGTTCGCGGAGC TGCGTGAGTGCATCCCCAATGTGCCCGCCGACACCAAGCTCTCCAAGATCAAGACTCTGCGCCTGGCTAC CAGTTACATCGCCTACTTGATGGACGTGCTGGCCAAGGATGCACAAGCAGGTGACCCCGAGGCCTTCAAG GCTGAACTCAAAAAGACGGATGGCGGTCGCGAAAGCAAGCGGAAAAGGGAGTTGCCTCAGCAGCCCGAAA GCTTCCCTCCTGCCTCGGGGCCCGGCGAGAAGAGGATTAAAGGGCGCACCGGCTGGCCTCAGCAAGTCTG GGCGCTGGAGCTAAACCAGTGAGACCAGGCCAACGCCGCCGGAGAACCCGGCCGAGGCTGGCCCACCTGG CTACGCGGAGAGGAGAGGAAAGGACGCAGCGGCTACGGCGGGTCTCCCGGTGCCCGCGCGCATTTCGCCC AGTCTCTGCGGAGCACCGGAGGGTCGGCGGTTGCAACTACCCACTAGGATCGCACGTGCAATGTCATTTG AATTTGTTAATACATAAGAGAAAGAGAAATTTATATATATGTTCACTTCCCGCCGAGGGCGGCTCTTGAA GGCTCGACTCAAGGTGGGACTGGAGAAGACCAAGCCCCCGAAGACACACCCTTCCAACCCATCTGGGTGG AACTCAACCCCAAAAGCCATGGTCGCGCCGCTCTCTGGCTCGCTCTCTCGTCCCCTTCTTGGCTCTGCGT CAGTACCCTGATGCCTTCTCGAGTTAAAATGCAATAAGAGTAATTGGTAGTTTCTAACCAGGAAGACTGA CGCTGCCTGGGTTGACCTCTCCTGAGCAGCGATGGTTTTCCTTCTTGTCGCTACACTTCCTACCTAGAGG GAAGGCGTTGCTCCCTTGCGCGGCACCTCTGCTTTCCAAGGGAAATCTTTCCCTTCCTTTCCCTTCCCTT CCCTCCCCTCGCCTCCTCCTTTCCCCTCTTCCGTCCTCTTACCTCCTCTTTCTCTTCCTTCCTTCTTCTT CATTTCTTTCCTTTTCCTTCTAAATAAAAGCGTTCCCATTCGTTGCTGAATTTGTAACGTATTTAAACTA CCTATCATGCCTGTTGCTCTCAGGTATACATCTCCCTACCCCCACCCTCTTTCTACCTCAAATCAGTGTG ACCACTCGCAGCCCCACCCTTTGCCAAAGCAGTATCTATGCTCTTGACTTAATAAAAAGTTTTCAGAGAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30022934 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30022934 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19597 same embryo
	EMAGE:19595 same embryo
	EMAGE:19593 same embryo
	EMAGE:19594 same embryo
	EurExpress:euxassay_004960 same experiment
	MGI:4825317 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source