EMAGE mouse gene expression spatial database

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EMAGE:19618

Gramd1c GRAM domain containing 1C ( MGI:2443024)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005110. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005110_01	euxassay_005110_02	euxassay_005110_03	euxassay_005110_04

euxassay_005110_05	euxassay_005110_06	euxassay_005110_07	euxassay_005110_08	euxassay_005110_09

euxassay_005110_10	euxassay_005110_11	euxassay_005110_12	euxassay_005110_13	euxassay_005110_14

euxassay_005110_15	euxassay_005110_16	euxassay_005110_17	euxassay_005110_18	euxassay_005110_19

euxassay_005110_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19618_wholemount_strong.wlz

19618_wholemount_moderate.wlz

19618_wholemount_weak.wlz

19618_wholemount_possible.wlz

19618_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19618_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

oral region

weak

regional

weak expression: see section 09 10 11 12

adenohypophysis

moderate

regional

moderate expression: see section 09 10 weak expression: see section 11 12

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 10 11 12

telencephalon ventricular layer

moderate

regional

moderate expression: see section 03 05 06 weak expression: see section 04 07 08 09 10 18 19 20

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 05 06 07 08 09

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12

pons ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11

spinal cord ventricular layer

moderate

regional

moderate expression: see section 09 10

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 12 13

liver

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7480
Entity Detected:	Gramd1c, GRAM domain containing 1C ( MGI:2443024)
Sequence:	sense strand is shown >T7480 CCACGCGTCCGAACTGAAGCTTCACACAGCACTGGCTTTTACGTGGCGAAGAGCCCCGTAAAGAGTAGGA AACTGAAGTACCGAGTTTGCTTAGCTGTTATCAAAGTTTTGAAGCTCTAACTGTGGATAAAGACTCCATA GAGTACTTAGAATACCAAGCTTTCTGGGCCAGAAATTGATCTGCTGACTTTTGACTCCTATCTGATTACT GCTTGGTTCATCTTTATTTTGCTCAAGTTATCTATCTGCAGGCTGAACCCAGAGCCGCTCCGCTCCTTTG CAGAGCCTGACCAGACAGGAGTTCTGGCAACTGCTCCAGCAGAACTATGGCACGGAGCTAGGCTTAAACG CTGAAGAAATGGAACACTTGTTATCAGTTGAAGAAAATGTGCAGCCAAGAAGTCCAGGAAGAAGCAGCGT GGATGACGCTGGGGAAAGAGATGAGAAGTTCTCCAAGGCCGTCAGCTTTACACAGGAGTCAGTTAGCAGA GCTTCAGAAACAGAGCCATTGGACGGGAACTCACCGAAAAGAGGACTAGGAAAAGAGGATTCCCAGAGCG AGAGAAATGTGAGAAAAAGTCCTTCGCTAGCTTCAGAAAAGAGGATAAGCAGAGCACCCTCCAAGTCACT GGACTTGAATAAGAATGAGTACCTTTCTCTGGATAAAAGCAGCACTTCAGATTCTGTGGACGAAGAAAAT ATTCCCGAGAAAGATCTTCAAGGAAGACTTTATATCAACCGTGTCTTTCACATCAGTGCTGAGAGAATGT TCGAACTGCTGTTCACTAGCTCACACTTTATGCAGAGATTTGCAAATTCTAGAAATATAATAGATGTGGT ATCTACCCCCTGGACAGTTGAATCTGGAGGCAATCAGCTGCGAACCATGACCTATACCATAGTCCTCAGC AACCCGCTAACTGGGAAGTATACTGCTGCCACAGAAAAGCAGACCCTGTATAAAGAGAGCCGGGAAGCAC AGTTCTACCTGGTAGACTCCGAAGTGCTGACACATGATGTGCCCTATCACGACTACTTCTACACTTTGAA CAGATACTGTATCGTGAGATCTGCAAAACAGAGATGCAGGCTGAGAGTCTCCACAGACTTGAAGTACAGG AAACAACCATGGGGCCTTATCAAGTCTTTAATTGAGAAGAATTCCTGGAGTTCACTGGAGAGCTACTTCA AAAAGCTTGAATCCGATTTGTTAATGGAAGAGTCTGTGTTGAGTCAATCCATTGAAGATGCTGGAAAACA TAGCAGCCTACGCCGGAGAAGGCGGACCTTGAACCGGACAGCAGAGCCCGTTCCAAAGCTGTCCTCTCAG CGCTCTTCCACAGATTTGGGCTTCGAGGCCAAAGTAGATGTTACAGGAAAGAGAAAGACCGTGGACAGTT ATGACACCGCCCTTATTGTGGTGATGAGCATATTCTTGCTGCTGCTCGTTCTGCTGAATGTGACACTATT TCTGAAGCTGTCAAAGATAGAACACGCTACCCAGTCCTTCTACCAGCTTCACCTCCAGGGAGAAAAATCT TTAAATTTAGTCTCTGACAGGTTCTCAAGAACAGAAAATATTCAAAAGAACAAAGATCAGGCCCACCGCC TAAAAGGAGTACTCCAAGATTCCATAGTGATGTTGGAACAGCTGAAGAGCTCACTCATTATGCTTCAAAA AACCTTTGATTTACTAAATAAGAACAAGTCTGGGGTGGCTGTGGAGAGCTAGTGCTGAAATGTGGAAACC AAAGAGACATCTGGACCTAGAAGGCAACATCTGGAAGGAATCGGAGGATGAAGACTGTCACTGTCATAAG GACATTTCTGATATTTTTATTACCAGGATTTCTACCAGGAAAGCTGTATTTAAATAACATTTACTCAATG ACTACTCTCCAATAGCCTTAAGACTCTATCTTTTCACTTCTTATAGATACTTTTAAGCTGTGAATTTCTC CAGTGAACCATGAAATATATTTTGGAATTGAAATTCTGTGACACAAAAGGGGAAGGAAGGCACTGTGGGT AGGATGGCTATTCTTCTATAGTGAGGCTTGCTACTTGATCCTTTAAATTTATGTTAGCACAGAGTCCCTG TGTGCCCAGGGTGGGGGTGGGGGGCTGCGTTTGTAACTGAGTTTAAGAGCCTAAAAAGGTAAGAAGAAAC CCCTCAATCATTTATCACCTTAAAGGATCGTTTTCCAAGTGCATACATTCCAGGCTTGTATCTTTAAATT TTACAGTGCCTTCCTTGACTTGGCCTCAAGGCCAATGGTCAGACCTTTTGTATGTCCGAAAGAGTCATTG GCCATGTTGTATCTGAATGAAGGGATATATATGTATATATATACACACATATATACATGTACATATATAT ACATATGTGTGTGCATATATGTATGTACGTATAAGTACCAAAGGTGAGATTATCCTTTATATATCAAAAA GACTGAACAGTAATTTAGGAGAAGGAAGTATATAGGACATCAATGCTCATAATGAACTACTTTCAGTTTT AGAAGTCGTAAGATTATTATAAAATCTGGGCATTCCATGACAGATCAAATTGAGTCCATGAGTAATGCTT AGATTCAGCTTAGCAACACCCATGATAGAGGTGGCCCATCAGGTAGAAAATAATTAATCACTTTTCAGTA GCAGCTAACTTGTCTTGCTGGCAAATTATAAGCTGGACACAAACTTGCACTCAGCAATAAAGACTGGGGG GCATGCAAGCTGACCGGAGTTGAGAGGCGCAAGCTTGGGCTCTCGGGAAAACCACTCAGGCACTTGTGTC AGCACCAGTGGTTTGTGAAAATAAGGACTTGGCGACTTGACTTGAAATTAGCTGCGTCCCCCGGGATTTA GGAAATGTTCAGGACGTATTTATTGTTTTGTTCTCTAGCTGTCTTTCTAGAACATGCACATATATTTATT TTTATTGAATATCCATTGCCTTTAGTCAATGTTTCCTTTCCCTCAGTGTCACCAAAGCCAAATGTCATTG CTAACAATGTTTTTGAGACGCCTGTAGGGAATAAAAATACTGGCTAAAGGGAAGGGTTTCTCTTCATGAA TCCTAACTAATCTCCCTGGGGCAGGGGGCTTCGGAGTAATACCTTCTGTTCTGTTCTGTTCTGTTCTGCT TTGTTTTGTTCTGTTCTGTTCTGTTCTGTTTTGTTTGTGATAAGAACATAGGAAGTCTCTGGCTGTGAGT TTGAGTGACTGGATTAGAAACTTCAGATTGTTAAATTTAGAAAAATGTTAACTGTCAAAGTGAATGCTTT TGTGGTTTCTCTTAAAGCACTTCACCATATACCTCAAAGATTACCAACGTCACTTTGTAGATAAAACACT AAATATGTACGTAGAATTAATGAGATCTTAATCTGTTATGTATGGATTGACTTCAATAAAACCGGAAATG TTTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4236901 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4236901 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19614 same embryo
	EMAGE:19616 same embryo
	EMAGE:19615 same embryo
	EMAGE:19617 same embryo
	EurExpress:euxassay_005110 same experiment
	MGI:4825212 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source