EMAGE mouse gene expression spatial database

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EMAGE:19654

Coro7 coronin 7 ( MGI:1926135)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009355. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009355_01	euxassay_009355_02	euxassay_009355_03	euxassay_009355_04

euxassay_009355_05	euxassay_009355_06	euxassay_009355_07	euxassay_009355_08	euxassay_009355_09

euxassay_009355_10	euxassay_009355_11	euxassay_009355_12	euxassay_009355_13	euxassay_009355_14

euxassay_009355_15	euxassay_009355_16	euxassay_009355_17	euxassay_009355_18	euxassay_009355_19

euxassay_009355_20	euxassay_009355_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19654_wholemount_strong.wlz

19654_wholemount_moderate.wlz

19654_wholemount_weak.wlz

19654_wholemount_possible.wlz

19654_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19654_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 08 09 moderate expression: see section 10 11 12

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

facial vii ganglion

strong

regional

strong expression: see section 17 19 moderate expression: see section 03 04 05

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 16

trigeminal v ganglion

strong

regional

strong expression: see section 17 18 19 20 moderate expression: see section 02 03 04 05 06 07 15 16

vagus x ganglion

moderate

regional

moderate expression: see section 06 15

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 16

trigeminal v nerve

moderate

regional

moderate expression: see section 08 15

spinal cord

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 09 10 13 moderate expression: see section 05 06 07 08 11 12 14 15

neural retina

strong

regional

strong expression: see section 01 moderate expression: see section 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9637
Entity Detected:	Coro7, coronin 7 ( MGI:1926135)
Sequence:	sense strand is shown >T9637 GCAGGCTGCTGAGGCGGCAGGCTGCAAGAGTCCAGGGCGGCTTCTCTTCATTCACCTAGTACCTGACGAC CCTCGTTCTCCAGCCTGAGCTTGCTCTGTCCCAGCTGCCATGAGTCGCTTCAAGGTGTCCAAGTTTCGGC ATATGGAGGCCCGGCCGTCCCGCCGAGAGGCCTGGATCAGTGATATTCGTGCTGTAACCACCCCCACATG CGGGAACCACATCAAATCAAGCTGCAGCTTGATCGCTTTCAACTCTGACCGCCCAGGTGTATTGGGTGTC ATATCTCTGGAAGGCCATGAGGAAAACAAGAGGCATGTGACCTACCTTGGCTGTCATTCAGACCTGGTCA CTGACCTGGACTTCTCCCCCTTTGATGACTTCCTCCTGGCCTCCGGTTCAGCTGACAGGACGATAAAGCT TTGGCGTCTGTCTGGCACTGGAGAAGCGCTTCCCTCTGTGCCTGGAGTGGTGCTGGGTCCTGAGGAGCTG CCAGTGGAGGTACTGCAGTTCCACCCCACTGTAGACGGTGTTCTGGTGAGCACAGCTGGCAAGACTGTGA AGGTCTGGGATGTAGCCAAGCAGCAGCCCCTGACAGAGTTGGAGGCCCACAAGGACCTGGTGCAGAGTGC TGTTTGGAGCCGGGATGGAGCCATTGTGGGCACAGCATGTAAGGACAAGCAGCTTCGGATCTTTGACCCC AGAGCAAGAACCCAGGCTTCTCAGAGCACACAGGCCCATGAGAACAACAGAGACATCCGGTTGGCATGGA CAGGCATTCAAGAGCACCTTGTGTCCACTGGATTCAACCAGATGCGTGAGCGAGAAGCGAAGCTCTGGGA TACCCGGCTCTTCAGCAGTGCCCTGGCCTCCGTCACCCTGGACACCTCTCCTGGGCCTCTGATACCTCTG CTGGATCCAGACTCTGGGCTATTGGTCCTGGCAGGAAAGGGTGAGAATCAGCTATACTGTTACGAGGTGA CCCCACAGCAGCCTGCACTGAGCCCAGTGACTCAGTGCATTCTGGAGAATGTACTACGAGGAGCAGCCCT TGTTCCCCGGCGAGCACTGGCTGTCATGAGTTGCGAGGTGCTCCAAGTCCTGCAGCTGAGTGACACAGCC ATCATACCCATCAGCCACCACGTGCCCCGAAAAGCTGTAGAGTTCCATGAGGACCTGTTTCCAGACACAG CCGGTTCTGTGCCTGCTTCTGATGCCCACATGTGGTGGGCTGGGGACAACCAGCAGGTTCAGAAGGTCAG CCTCAACCCAGCTCGCCGACCCCACCCTTGCTTCACGTCCTCCCTGGTGCCCACTATGGAACCGGCCCCT GACATGGTCCAGCCTGCAGAGATGCCCAGAGCTGACACAGACCTAAGTGAGGGCTTTTCTTCCCCTAGCT CACTGATGTCACCCTCCACACCATCCAGTCTGGGGCCTTCACTCTCCAGCACGAGCGGCATTGGTACCAG CCCCAGTCAGAGGTCCCTTCAGAGTCTGCTGGGCCCCAGTTCTAAATTTCGCCACACTCAGGGCAGCCTC CTCCACCGCGACAGTCACATCACCAACCTTAAGGGACTCAACCTCACAACACCTGGTGAGAGTGATGGCT TCTGCGCTAACCGGCTGCGTGTGGCTGTTCCCCTGCTCAGCAGTGGTGGGCAGGTAGCTGTGCTTGAGCT GCAGAAGCCAGGCCGCCTGCCTGACACAGCATTGCCTACACTTCAGAATGGGACAGCTGTAATGGATCTG GTCTGGGATCCCTTTGACCCACACCGGCTTGCTGTGGCTGGGGAGGATGCCCGGATCCGACTATGGCGGG TGCCCCCTGGTGGCCTCGAGAATGTTCTCACCACACCTGAGACTGTGCTCACAGGCCACACAGAAAAGAT CTACTCTCTGCGCTTCCACCCCCTGGCTGCCGATGTACTGGCTTCCAGTTCCTATGATCTAACCGTTCGC ATCTGGGACCTTCAGACTGGAGCTGAGCGGCTGAAACTGCAGGGCCATCAGGACCAGATTTTTAGCTTGG CCTGGAGTCCAGATGGAAAGCAACTGGCCACCGTATGCAAGGATGGGCATGTGCGGGTCTATGAACCCCG CAGCAGCCCTTTACCCCTACAGGAAGGCCCAGGACCTGAAGGTGGCCGCGGAGCCCGCATTGTCTGGGTG TGTGATGGTGGTTGCCTGCTGGTTTCTGGCTTTGACAGCCGGAGTGAACGCCAGCTACAGTTGTACATAG CTGACGCCCTGGCACAAGGCCCTTCAGCACTGCTGGGCCTCGATGTAGCTCCCTCGACCCTGCTTCCCAG CTATGACCCTGACACAGGCCTGGTGCTTCTCACTGGCAAGGGTGATACCCGTGTGTTCCTGTATGAGGTG CTCCCGGAGGCCCCTTTCTTCCTGGAGTGCAACAGCTTCACATCACCTGACCCTCACAAGGGTTTTGTCC TTCTGCCCAAGACTGAGTGTGACATTCAGGATGTGGAGTTTGCCCGATGCCTGCGGCTGCGCCAGACCTC CTTGGAGCCTGTTGCTTTCCGGCTGCCCCGGGTCAGAAAAGAATTCTTCCAGGATGATGTGTTCCCGGAC ACAGCTGTGACCTGGGAGCCAGCACTCAGTGCCAAAGCCTGGTTTGAGGGTGCTAATGGACAGCCCCGGC TACTGAGTTTGCAGCCCCCTGGCATGACCCCAGTGAGCCAGGCCCCCCGTGAGGTCCCTGCCCGAAGAGC CCCATCTTCAGCACAATACTTGGAAGAGAAGTCTGACCAACAAAAGAAAGAAGAGCTGCTGAATGCCATG GTAGCAAAGCTGGGGAACCGAGAAGACCCACTGCCACAGGACTCCTTTGAAGGGGTGGATGAAGATGAGT GGGACTAGCCTGCACGCGAGGCACGCCTAATCACAGCTGCCACTGCCACCTCCTACAACTACCTGCTGCC CTCCACACCTAAGATCTCTGCAACCTGGACAATGCTGCACTGGCCCTGGCACGCACATCCAGCTCAGAAG CCCTGGGTGTCACGCAAGCCTCAGGCACTTCCACAGCTGTGGGGCCTCCATGCAGCTGCAGAGACTGCCT GCTCCTTAACCCTCTGGAGCCAACTGTTTATCTTCAGCTGCCTGTTAGCATGTGACACCAGTGGGTGGGA GGTAGGTGTCAGTAGCATGTCTCTGGGCCAACTCGGGATGCCCTGTCTCTCCCAGTCTTTCTGCTGGTTT CCTCTAATATCTTCAGTTACTACAAGCCAAGCTGTCTTGCCTACTGCACCAGCCCCAGTCAAGGACACTG GGGGCTGGTCCCCCACCAAGCTGTTGGGAGCCGCTTTCTCCTTAGGGGCCTAGGACCATTTGTCCTGCTT CTCCTGGCTTGAGGAGAAAGGGTCTCATGGCGCTAACAGCCTCATTACAGTGGTGTGGACCCCATAACAA ACAGCCTATATAGGATATGCTCTGGATGTTTCCAGCAATTAAACTTTTCTAAGCTGGCAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6536106 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6536106 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19657 same embryo
	EMAGE:19656 same embryo
	EMAGE:19658 same embryo
	EMAGE:19655 same embryo
	EurExpress:euxassay_009355 same experiment
	MGI:4824013 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source