EMAGE mouse gene expression spatial database

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EMAGE:19677

Ptpn9 protein tyrosine phosphatase, non-receptor type 9 ( MGI:1928376)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006132. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006132_01	euxassay_006132_02	euxassay_006132_03	euxassay_006132_04

euxassay_006132_05	euxassay_006132_06	euxassay_006132_07	euxassay_006132_08	euxassay_006132_09

euxassay_006132_10	euxassay_006132_11	euxassay_006132_12	euxassay_006132_13	euxassay_006132_14

euxassay_006132_15	euxassay_006132_16	euxassay_006132_17	euxassay_006132_18	euxassay_006132_19

euxassay_006132_20	euxassay_006132_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19677_wholemount_strong.wlz

19677_wholemount_moderate.wlz

19677_wholemount_weak.wlz

19677_wholemount_possible.wlz

19677_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19677_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8553
Entity Detected:	Ptpn9, protein tyrosine phosphatase, non-receptor type 9 ( MGI:1928376)
Sequence:	sense strand is shown >T8553 AGATGCTCAGTTTCTCAATGGAGCCGTGGGGGGCTCGGGCGGACGCGGCGCAGGCCGCAGCGCTGGCCCT GAGCCGGGAGGGGGACGCCTTTTCCTCGCTCGCTCGCGCCCGCCCTCCCTGTCTCCGCGGCGGCTTTTGA GGAACTGGGCCGCGAGGCCACGGAGCAGTTCGCAGGGCCCGCATCCCCGTGGACGCCCGGGCTGGGCGGT CTCGAGGCGCCGGCGAGCTCTGCCCCGGGGGTTAAGCGGCTCGGAAGGCCGACGGCCTCCAACTCCCGCC AGGCGCCTTCGCGGTGGCTGTAAAAGTTGTGGACTCCTAAAAAAGAAAGAAAGCCAAGCAGACCTCGAAC TGCTGTTAGTTCCTCGCCCGCACTTTCTCCTCCTCAGGACAGGAGGGGTGGCGTGAGGAAAGGAGTGAAG CAAACAGGAGGCATTTCCTCTCCGCGGGGAGCGACAGCGGGCTCTCCCCGGCCAGCAGGAGCGGCGTTGG CAGCCGCCGCGGGCGCCGGGCGCTCGCACCATGCGCGAGCCGACCGAGAGCTGCTGAGGCGCCGGGCCAC AGAAGCGGAGCGCGAGTGCAGCGGGTTCTGGCGCTCGCCCACCAGACCTGCCCCGGCGGCGGCGGGATGG AGCCGGCGACCGCGCCCCGGCCGGACATGGCGCCGGAGCTGACCCCCGAGGAGGAGCAGGCTACCAAGCA GTTTCTTGAAGAGATCAACAAGTGGACAGTACAGTATAATGTTTCTCCACTCTCCTGGAATGTGGCTGTC AAGTTCCTCATGGCCAGAAAGTTTGATGTGCTCCGTGCTGTAGAGTTGTTCCACTGCTACAGAGAAACAA GAAGAAAGGAAGGCATTGTGAAGCTGAAACCTCATGAGGAGCCTCTCCGTTCTGAGATCCTCAGTGGGAA GTTTACTATCTTGAATGTTCGAGACCCAACTGGAGCCTCCATTGCCCTCTTCACTGCCAGGTTACACCAC CCCCACAAGTCTGCCCAGCACGTGGTGCTCCAAGCTCTGTTTTATCTGCTGGACAGAGCTGTGGACAGCT TTGAAACTCAAAGAAATGGACTGGTGTTCATCTATGACATGTGCGGGTCTAATTATGCCAACTTTGAGCT AGATCTTGGCAAGAAAGTCCTAAACCTGCTGAAGGGAGCATTTCCAGCTCGACTGAAGAAAGTGCTGATT GTAGGAGCACCCATATGGTTCCGAGTGCCCTATTCAATCATCAGCCTGCTCTTGAAGGATAAAGTCCGGG AAAGGATTCAGATATTAAAGACATCTGAAGTTACCCAGCACCTGCCTAGGGAGTGCCTTCCAGAAAATCT GGGTGGGTACGTCAAAATTGACCTGGCTACTTGGAATTTCCAGTTCCTGCCCCAAGTAAACGGCCACCCA GATCCCTTCGATGAGATCATCCTGTCTTCCCTACCTCCTGCCTTAGACTGGGACTCAGTACATGTTCCAG GTCCTCATGCCATGACCATCCAAGAGTTGGTAGACTATGTTAACACCAGGCAAAAGCGGGGCATCTATGA GGAGTATGAAGACATTCGTCGTGAGAACCCTGTTGGCACTTTCCACTGTTCCATGTCTCCAGGAAACCTA GAAAAAAACAGATATGGAGATGTACCCTGCCTAGACCAAACCCGAGTGAAGCTGACAAAACGGAGTGGCC ACACTCAGACAGATTACATCAATGCCAGTTTCATGGATGGTTACAAGCAGAAGAATGCTTATATTGGTAC ACAAGGTCCTTTGGAGAACACCTATCGTGACTTCTGGCTTATGGTTTGGGAGCAAAAAGTTTTGGTGATT GTCATGACTACCCGCTTTGAGGAAGGCGGCAGGAGAAAATGTGGCCAGTACTGGCCTTTAGAAAAAGACT CCCGGATCCGATTTGGCTTCCTCACGGTGACTAATCTAGGAGTGGAAAACATGAACCATTATAAGAAAAC AACTCTAGAAATTCACAACACAGAGGAGCGGCAGAAACGCCAGGTTACCCACTTCCAGTTCCTAAGCTGG CCAGATTATGGTGTCCCTTCCTCAGCTGCTTCCCTCATTGACTTCCTGAGAGTGGTCAGAAACCAGCAGA GTATGGCTGTAGGCAACCTGGGAGCTCGCTCCAAAGGGCAGTGCCCTGAGCCACCCATTGTGGTCCACTG CAGTGCTGGCATCGGCAGGACAGGTACCTTCTGCTCACTGGACATCTGCCTGGCACAGCTGGAGGAGCTT GGCACCCTTAATGTGTTCCAGACGGTGTCACGCATGAGGACCCAAAGGGCCTTCAGTATCCAGACCCCTG AGCAGTACTACTTTTGCTACAAGGCAATTCTGGAGTTTGCAGAGAGGGAAGGCATGGTTCCCTCTGGTCA CAGCCTGTTGGCCATGGACGGTCAATAACTGCTCCACAAGTTTCCACCTGCTGGTCAGCCTTCCTTAAAC TACCCTGGACACTGCTGAGCCTATAGGTTGCCATCAGTTATGCTGAAGCCATGGATCAACCTCTTTCCTG TGTCTCCCAGCGCACATGTGCACGCAAGGCAGCCTTTCCCTTTGGCTAGATAAATGTGGTGAACTTGCCA CTAGAAAGGGCCAGCAGCAATGTGTTCTTAATTCCTAGCACCTGCTATCGAACTGTGCCTTATCAAAACC AAATTGTGGCTATTGATTTTTGCCAGGGTCCCTGAGGTAAGTGGGGAAGGACCCCTCCCCCCACCCCCAT CTCCCAGTTCCTCTGGACATCTCCTCCTTCCCTTTGCTATGATTTGACAGGGAGGTCTGGTGAGCATTCA CCTTCCTACCCAGAAAGCTTGACCAAGTTACATATTCTAGAGAATGTCCCGAGTGCCAAGCACGTTTATA CATAGGGCGTGTATTCCAGTCTTTTCTGTCATTCTGTGTGTGTGTGTGTGTACGTATGTGCACTTACGTG TATGGATCCATATGTATGTGTGTACATAATATATACTCTGTGATAATTATGGCCGTATACTATAAATACA TATACACACAGATACTCTTTGTAGAAGTCTCTGGGGATTTATGTTGCAGTTCAGCATTCCTTGCTTCTTG GACCCTGCTGATTATTCTGGACTGGCTGGGGTTAGGGATCATGGCTCATGTTGTCAGACAGCAGAGTGAT AGAAGAAAACACATACTTTTTTCCTGGGCCACAGGTCTTCTTCCTTAATGAAAATTTTCCCTCTGGACTC TATGCTTGTTGGAGCCAAAAATACTGACTTATGGTAACACTGGTTCAATTTAGCATGGGTGTCTTTCATC TAACAAATTCTCTGTTGTCTTTGCTGATTTGGGGAACAGACCTCCACTGTGATTCCAGACTCTGTCTTAT GACCCCATTGATGGGGACCAAGGCTAGAAATAAATTTACAGAGCTGCCAGAGTCCAAAGTAATCGGTACG AAGTTTTAAAAGGAATAAAGGAGAGCCCAGAGTGAACAAGAGGAACCTACCATGAAATCCCCTTCTTGGG AGGACAAACTGGGGCCTCTTTTTCTTGTGTTGGAAGACACCTTTCTTTATGCAACTCTTCCTTGTCTACT GAATGCCCAGCTCTCCCTGCCTGCTAGCCTGCCAGATCCTAGACTGCAAACTGGGTGGGCCTATGTCTTT ATAAAACATAAACTGGGTGGGTCTATGTCTTTATGAAGCATAATCCCTTTGGCTGCATGAGTCCGTTCCT TGGAATCACTGGTCCACTGCCCGTACAACTTGTTCCTGTCTTTTCCCAGTCTGATTCGCCAGGAATGGAA TCAAGAGTTCCCAACACTGTGTTCCCACAAAACCCTACAGAACCCCTAAACCTTCCCTTACTCCACACGA TGAGGTTGGCAGCTGGTCAGAGCTCAATAATTTTATACTGTAATAAGATCTTTGAATGTGTATATTCTTA TTTTTTACAATCATTTCATTTTGTATTTAACATCAATGGACTGAGTCAAGATTCAGTAAATAATTGTAAT GTTCATATGCGATATCTTATAGTGGTACAATTTTGTATTTACCTATAAATATACCAACATGATTAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5708923 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5708923 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19680 same embryo
	EMAGE:19678 same embryo
	EMAGE:19679 same embryo
	EMAGE:19681 same embryo
	EurExpress:euxassay_006132 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source