EMAGE mouse gene expression spatial database

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EMAGE:19680

Fgfr3 fibroblast growth factor receptor 3 ( MGI:95524)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006120_01	euxassay_006120_02	euxassay_006120_03	euxassay_006120_04	euxassay_006120_05

euxassay_006120_06	euxassay_006120_07	euxassay_006120_08	euxassay_006120_09	euxassay_006120_10

euxassay_006120_11	euxassay_006120_12	euxassay_006120_13	euxassay_006120_14	euxassay_006120_15

euxassay_006120_16	euxassay_006120_17	euxassay_006120_18	euxassay_006120_19	euxassay_006120_20

euxassay_006120_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19680_wholemount_strong.wlz

19680_wholemount_moderate.wlz

19680_wholemount_weak.wlz

19680_wholemount_possible.wlz

19680_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19680_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

spotted

strong expression: see section 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8552
Entity Detected:	Fgfr3, fibroblast growth factor receptor 3 ( MGI:95524)
Sequence:	sense strand is shown >T8552 CGGGAGCAGCTGGCGCCGTGCGATCCACTCCGGCGGGGGGACTCAGTGGTGGGCGGCCGGCCACTGGGAC AGAGGAGACCCTGGAAAAGCGGGCCGAGAGACGGAGCCGCGCGTGTCTCCACAGAGGCGTTCTCCCACCG GCGCCGGAGCCGGGCGTGGGGGGTTGCAGCATGCCCGCGCGCGCTGCTTGAGGACGCCGCGGCCCCCGCT CTGGAGCCATGGTAGTCCCGGCCTGCGTGCTAGTGTTCTGCGTGGCGGTCGTGGCTGGAGCTACTTCCGA GCCTCCTGGTCCAGAGCAGCGAGTTGTGCGGAGAGCGGCAGAGGTTCCAGGGCCTGAACCTAGCCAGCAG GAGCAGGTGGCCTTCGGCAGTGGGGACACCGTGGAGCTGAGCTGCCATCCTCCTGGAGGTGCCCCCACAG GGCCCACGGTCTGGGCTAAGGATGGTACAGGTCTGGTGGCCTCCCACCGCATCCTGGTGGGGCCTCAGAG GCTGCAAGTGCTAAATGCCTCCCACGAAGATGCAGGGGTCTACAGCTGCCAGCACCGGCTCACTCGGCGT GTGCTGTGCCACTTCAGTGTGCGTGTAACAGATGCTCCATCCTCAGGAGATGACGAAGATGGGGAGGACG TGGCTGAAGACACAGGGGCTCCTTATTGGACTCGCCCGGAGCGAATGGATAAGAAACTGCTGGCTGTGCC AGCCGCAAACACTGTCCGCTTCCGCTGCCCAGCTGCTGGCAACCCTACCCCCTCCATCTCCTGGCTGAAG AATGGCAAAGAATTCCGAGGGGAGCATCGCATTGGGGGCATCAAGCTCCGGCACCAGCAGTGGAGCTTGG TCATGGAAAGTGTGGTACCCTCCGATCGTGGCAACTATACCTGTGTAGTTGAGAACAAGTTTGGCAGCAT CCGGCAGACATACACACTGGATGTGCTGGAGCGCTCCCCACACCGGCCCATCCTGCAGGCTGGGCTGCCG GCCAACCAGACAGCCATTCTAGGCAGTGACGTGGAGTTCCACTGCAAGGTGTACAGCGATGCACAGCCAC ACATCCAGTGGCTGAAGCACGTGGAAGTGAACGGCAGCAAGGTGGGCCCTGACGGCACGCCCTACGTCAC TGTACTCAAGACTGCAGGCGCTAACACCACCGACAAGGAGCTAGAGGTTCTGTCCTTGCACAATGTCACC TTTGAGGACGCGGGGGAGTACACCTGCCTGGCGGGCAATTCTATTGGGTTTTCCCATCACTCTGCGTGGC TGGTGGTGCTGCCAGCTGAGGAGGAGCTGATGGAAACTGATGAGGCTGGCAGCGTGTACGCAGGCGTCCT CAGCTACGGGGTGGTCTTCTTCCTCTTCATCCTGGTGGTGGCAGCTGTGATACTCTGCCGCCTGCGCAGT CCCCCAAAGAAGGGCTTGGGCTCGCCCACCGTGCACAAGGTCTCTCGCTTCCCGCTTAAGCGACAGGTGT CCTTGGAATCTAACTCCTCTATGAACTCCAACACACCCCTTGTCCGGATTGCCCGGCTGTCCTCAGGAGA AGGTCCTGTTCTGGCCAATGTTTCTGAACTTGAGCTGCCTGCTGACCCCAAGTGGGAGCTATCCAGGACC CGGCTGACACTTGGTAAGCCTCTTGGAGAAGGCTGCTTTGGACAGGTGGTCATGGCAGAAGCTATTGGCA TCGACAAGGACCGTACTGCCAAGCCTGTCACCGTGGCCGTGAAGATGCTGAAAGATGATGCGACTGACAA GGACCTGTCGGACCTGGTATCTGAGATGGAGATGATGAAAATGATTGGCAAGCACAAGAACATCATTAAC CTGCTGGGGGCGTGCACACAGGGTGGGCCCCTGTATGTGCTGGTGGAGTACGCAGCCAAGGGCAATCTCC GGGAGTTCCTTCGGGCGCGGCGGCCTCCAGGCATGGACTACTCCTTTGATGCCTGCAGGCTGCCAGAGGA ACAGCTCACCTGCAAGGATCTAGTGTCCTGTGCCTACCAGGTGGCACGGGGCATGGAATACTTGGCTTCT CAGAAGTGTATTCACAGAGACTTGGCTGCCAGAAACGTCCTGGTGACCGAGGACAATGTGATGAAGATTG CGGACTTTGGCCTGGCTCGAGATGTGCACAACCTGGACTACTACAAGAAGACCACAAATGGCCGGCTACC TGTGAAGTGGATGGCACCAGAGGCCCTTTTTGACCGAGTCTACACCCACCAGAGTGATGTTTGGTCTTTT GGTGTCCTCCTCTGGGAGATCTTTACGCTGGGGGGCTCACCGTATCCTGGCATCCCAGTGGAAGAGCTTT TCAAGCTGTTGAAAGAGGGCCACCGCATGGACAAGCCAGCCAGCTGCACACATGACCTGTACATGATCAT GCGGGAATGTTGGCATGCGGTGCCTTCACAGAGGCCCACCTTCAAGCAGTTGGTAGAGGATTTAGACCGC ATCCTCACTGTGACATCAACCGACGAGTACTTGGACCTCTCCGTGCCGTTTGAGCAGTACTCGCCAGGTG GCCAGGACACGCCTAGCTCCAGCTCGTCCGGAGATGACTCGGTGTTCACCCATGACCTGCTACCCCCAGG TCCACCCAGTAACGGGGGACCTCGGACGTGAAGGGCCAACAGTCCCACAGACCAAGCCCCAGGCAATGTT TACGCGGACCCTAGCCCGCCCTGCTACTGCTGGTGTGCAGTGGACCCTAGCCAGCCCAGTGCAATGGGCC AACAGTAGACAAGACTTCCTGCGTGTTTATCCTTGGCTCCTGGGTGCAGAGGCCCCTTGGGAACATGCAC TGCTGTAGAGTAATCTCCTGACTGGCCAGGGCCAGGAGCACCAAACAAGAATGTAAGAGGCCCACCCTGT GCAACCCTGGGGTTCTGGCCCTCTCATTTCCCACTGCTACCTTCCAGGGACCATTGTGGAGAGGGCTAGA CTCCATGTCCAGAGTGGGCCTTGGCCTTCTTGGTGCCCCAAGCTGAGCCTACAGGGAGGCTCTGCTCTGT GTGGCAAACCTCTCTCCTACATGGCACCTTGTGCCTGGGGGTGTCATAGCTCGACATCTCCAGGCTGCCT GCTTTCCACCCTGCCCCTCAGAGACAAATTACGGGTACCTGAAGGGGGGGCATAATGTCTATCAGAAAGG TTTATTCCAGAGGAAAATGTACATTTATATAAATAGATGTTGTGTATGATATAAATATATACATACATAT ATATAAGAATATCTATATGGAAAAAGGCAAAGTTGAGGCCCAAGGGAGCAAGATACTCCATGGGTCTCAC TAGGAAACTGGCAAGAGCAGGCTGAGAAGCAAGGGGCTTTTCTGGCACGGCAGTTTTGTTTGTACTGGAC CTGTATATTTGTAAAGCTATTTATCAACCCCCAGAGCGCCAGTCCCCGACCCCAGGTTCATAGCGTTTAG TCCCAGGGTATTGCAGCCATCTTAAGTTGTAACTTATTAACAGCGGAAGAGGTTCATGCTGGATTTAGGG AATTGCTGAGAACGTGCGTCTGGCCTCCACCAGGCTGGCCGTGGCCCCTTGGCGCTTGAATGGCTCTCCT AGTCAGAGCTGGCTCCAGGGAGCATTTTCTGTTGCCTTTGGCCCTCTTTTGTGGGGGATTAGATTTATAT AGGAACTTTCTTTAGGAGATGTTTAAAAATTTTAAGGTGAACTGGTATTTTTCATACAGATTATTCTAAT TGCTATGTATTCCAGGCAGGAGCCTGTGCCCAGGGAAGGGCTGGCCCTGCAAGAAGGTTCAGATGTTAAT AGTTATCTGTTACAAGTTTATCTATCTATAATTTATTGAGTTTTTACAAGTTGTTTTGCTGTAGGCTTAA CACTTCCTATGCAGTGCTTCTAGACTTTTATAGCCTAGACTGCTACCTTTCAAAGCTTGGGAGACAGTGG TAAATGCAATTTTGTTACTTTTGTACTGTCACTGGGCCCTAGGCTTGGGTGGCTGTCCCTTGCCTGTCAA CCAGCAGGGTCAGGACAGTGGCTCAGGGTGACTTTCTTGGGGCCTAGCACATGGTTTGTCAGCCCACACT GGCAGATGTGGTTTTGTTAACACAACCAACTTACTTTCCAAAAAATAAAGAGATAACTGGTTCCAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5708838 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5708838 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19678 same embryo
	EMAGE:19679 same embryo
	EMAGE:19677 same embryo
	EMAGE:19681 same embryo
	EurExpress:euxassay_006120 same experiment
	MGI:4824848 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source