EMAGE mouse gene expression spatial database

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EMAGE:19683

Iah1 isoamyl acetate-hydrolyzing esterase 1 homolog (S. cerevisiae) ( MGI:1914982)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005176. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005176_01	euxassay_005176_02	euxassay_005176_03	euxassay_005176_04

euxassay_005176_05	euxassay_005176_06	euxassay_005176_07	euxassay_005176_08	euxassay_005176_09

euxassay_005176_10	euxassay_005176_11	euxassay_005176_12	euxassay_005176_13	euxassay_005176_14

euxassay_005176_15	euxassay_005176_16	euxassay_005176_17	euxassay_005176_18	euxassay_005176_19

euxassay_005176_20	euxassay_005176_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19683_wholemount_strong.wlz

19683_wholemount_moderate.wlz

19683_wholemount_weak.wlz

19683_wholemount_possible.wlz

19683_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19683_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 08 09 10 11 12

pancreas

moderate

regional

moderate expression: see section 07 08 09 10 11 13 14 15

thyroid gland

moderate

regional

moderate expression: see section 08 09 12

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 17 weak expression: see section 06 07 16

spinal cord

moderate

regional

moderate expression: see section 09 11

metanephros

moderate

spotted

moderate expression: see section 03 04 05 06 07 08 14 15

testis

moderate

spotted

moderate expression: see section 03 04 05 06 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9631
Entity Detected:	Iah1, isoamyl acetate-hydrolyzing esterase 1 homolog (S. cerevisiae) ( MGI:1914982)
Sequence:	sense strand is shown >T9631 CTCCACATCCGGCGCAGCCCTGCGGGGTTTTGGGCTTTCTACCATGTCGCTGTGCGAGCGAGCTGCTAGC GGGAGCGCTCTGCTCTGGCCTCGCGTGTTGCTCTTCGGGGACTCCATCACGCAGTTTTCTTTCCAGCAGG GTGGATGGGGCTCGTTGCTGGCTGACAGACTAGTCAGAAAGTGTGATGTTCTGAATCGTGGATTTTCAGG TTATAACACCAGATGGGCCAAGATTATCCTCCCAAGACTTATCAGGAAAGGGCCTGGGATGGAAAACCCA GTGGCCGTTACAATCTTCTTTGGTGCCAATGACTCTTCACTCAAAGATGAGAACCCCAAGCAGCACGTCC CCTTGGATGAGTACAGCGCAAACCTGAGGGACATGGTGCAGTACCTGAGGTCTGTGGACGTCCCCAGGGA GCGGGTCATCCTTATCACGCCACCCCCGCTCTGCGAAGCCGCCTGGGAGAAGGAGTGCGTCCTGAAGGGT TGCAAATTAAACCGCCTGAACTCAGTTGTTGGTGAATATGCAAATGCGTGTTTACAAGTAGCCCGAGACT GTGGGACTGATGTCCTTGACCTGTGGACCCTGATGCAGAAGGACAGCCAGGACTTCTCATCCTATTTATC GGATGGCCTGCATTTATCACCAATGGGAAATGAGTTTCTGTTCTTAAACCTCTGTCCACTGCTGGACAAG AAGGTCTCATCTCTGCCGTGGCTGCTTCCTTACTGGAAGGATGTGGAGGAAGCAAAGCCCGAGCTGAGTC TGCTGGGAGATGGAGACTATTAGCTTGGGAGATGTTCCCAAAAATTAATGGCTCGTATCATTCATGTTGG TAGCCACAAACATTCTGTGAACATGTGCGTAGCTTTATTAGTAATTCACAGACATCAAGAGATTGATTAA ATGTCTTTTCCAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6533986 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6533986 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19682 same embryo
	EMAGE:19686 same embryo
	EMAGE:19684 same embryo
	EMAGE:19685 same embryo
	EurExpress:euxassay_005176 same experiment
	MGI:4825492 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source