EMAGE mouse gene expression spatial database

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EMAGE:19703

Cep57 centrosomal protein 57 ( MGI:1915551)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006338. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006338_01	euxassay_006338_02	euxassay_006338_03	euxassay_006338_04

euxassay_006338_05	euxassay_006338_06	euxassay_006338_07	euxassay_006338_08	euxassay_006338_09

euxassay_006338_10	euxassay_006338_11	euxassay_006338_12	euxassay_006338_13	euxassay_006338_14

euxassay_006338_15	euxassay_006338_16	euxassay_006338_17	euxassay_006338_18	euxassay_006338_19

euxassay_006338_20	euxassay_006338_21	euxassay_006338_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19703_wholemount_strong.wlz

19703_wholemount_moderate.wlz

19703_wholemount_weak.wlz

19703_wholemount_possible.wlz

19703_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19703_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9677
Entity Detected:	Cep57, centrosomal protein 57 ( MGI:1915551)
Sequence:	sense strand is shown >T9677 GGACGCAAGTAAGAAAGATCCTAAAGTCGTCCGGAAAGGTTTTGAGGAGTACTGAAAGGGAAGAGGCTGA AGGCCGGGAGGGCTGCTGTTTTCTTTTGTGAACTGGCATCCCAGTTGGTAGCTGCAGAAAATAAATGTAA TCTTCTAGAAAAACAATTGGAATACATGCGAAATATGATAAAGCATGCAGAAATGGAGAGGACATCTGTG TTAGAAAAGCAGGTTTCTCTAGAAAGAGAACGGCAGCATGATCAAACCCATGTTCAGAGCCAGCTGGAAA AACTGGATCTTCTTGAACAGGAATACAACAAACTCACCGCCATGCAGGCCCTTGCAGAAAAAAAGATGCA GGAGTTGGAGTCCAAACTCCGTGAAGAAGAGCAGGAAAGGAAGCGGATGCAGGCCCGGGCAGCAGAGTTA CAGAGTGGTCTAGAAGCAAATAGACTTATTTTTGAAGATAAGACTACTTCATGTGTTTCAACCAGCACTA GAAAAATAAAAAAAAAGAAGTCAAAACCACCAGAAAAGAAAGGTTCTAGGACCTATTTTGGTGCACAGCC ACATTACAGATTGTGCTTAGGTGATATGCCTTTTGTTGCTGGAACGTCCACCAGTCCCAGCCATGCCGTG GTAGCCAATGTTCAGCATGTCTTGCATCTGATGAAACACCACAGTAAAGCCCTGTGCAATGATCGAGTTG TTAACAGTGTTCCCTTGGCAAAGCAAGCTTGCTCACGAGTTAGTAAGAGTAAGAAGTCAGTGGTGCCTCC TTCCAGCAGTGTTAATGAAGAACTTTCGGATGTCTTGCAGACTTTACAGGATGAATTTGGGCAAATGAGC TTTGATCATCAGCAGCTTACTAAACTCATACAGGAATCACCAACTGTGGAACTAAAAGACAACCTGGAGT GTGAATTGGAGGCATTAGTGGGAAGAATGGAAGCGAAGGCCAATCAGATAACTAAAGTTCGAAAATACCA AGCCCAGCTGGAGAAACAAAACATAGATAAGCAGAAGAAGGAATTAAAAGCTAACAAAAAGACTCTTGAT GAAGAAGGGAACAGCAGCGGTCGTTCATCTGGAGTCCCAAGGACCGCAAGTAAGAAGGATCTGGCCAAAC AGAGACCTGGAGAAAAAAGCAGGAAAAATCTTCAGTTACTGAAGGACATGCAGACCATACAGAATTCACT GCAGAGCAGTAATCTGTGTTGGGATTACTGACACCAGGTCATAAATGATGTACCTCACTGGTCATAAACG ATGTACGTACTGGACATGAGCAGACTTTCCAGGTCTCATGCTTGCTAAGCTGCCATTACTGGCAGGTGTT AGGGGCCAAGCTTCATTACAGTCTATTGTGCTATGTAGCACAACTGATGGAGATGGAGTTCTGCGGCAGA GAAGCCTCACTGTCTTTGAATTTGCTGTACTCATTCACTGCACTTTGTAAACAAATGACCAGTTTTTTAC TCATGGGTGTGTTTTTTAAACTAGGTATATATGTAAATTGGGTTTGAAAATTCAGAATATGAGATAGTCC CCATAGTCAGTGCTTGAAGTCTTGTTTTTTAAATCTTCCCACAGCATGAAGTGACCACTACTCTCCCTTC TCCAGATGACCATTAAATGTACTGTTTTCAAGAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6703205 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6703205 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19704 same embryo
	EMAGE:19705 same embryo
	EMAGE:19702 same embryo
	EMAGE:19701 same embryo
	EurExpress:euxassay_006338 same experiment
	MGI:4823816 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source