EMAGE mouse gene expression spatial database

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EMAGE:19706

Cadm1 cell adhesion molecule 1 ( MGI:1889272)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006162. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006162_01	euxassay_006162_02	euxassay_006162_03	euxassay_006162_04

euxassay_006162_05	euxassay_006162_06	euxassay_006162_07	euxassay_006162_08	euxassay_006162_09

euxassay_006162_10	euxassay_006162_11	euxassay_006162_12	euxassay_006162_13	euxassay_006162_14

euxassay_006162_15	euxassay_006162_16	euxassay_006162_17	euxassay_006162_18	euxassay_006162_19

euxassay_006162_20	euxassay_006162_21	euxassay_006162_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19706_wholemount_strong.wlz

19706_wholemount_moderate.wlz

19706_wholemount_weak.wlz

19706_wholemount_possible.wlz

19706_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19706_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8878
Entity Detected:	Cadm1, cell adhesion molecule 1 ( MGI:1889272)
Sequence:	sense strand is shown >T8878 GTGCCCGACATGGCGAGTGCTGTGCTGCCGAGCGGATCCCAGTGTGCGGCGGCAGCGGCTGTGGCGGCGG CGGCGGCGCCTCCAGGGCTCCGGCTCCGGCTCCTGCTGTTGCTCCTTTCGGCCGCGGCACTGATCCCCAC AGGTGATGGACAGAATCTGTTTACTAAAGACGTGACAGTGATTGAAGGAGAAGTGGCAACCATCAGCTGC CAGGTCAATAAGAGTGACGACTCAGTGATCCAGCTCCTGAACCCCAACAGGCAGACCATTTACTTCAGGG ACTTCAGGCCTTTGAAGGACAGCAGGTTTCAGCTGCTGAATTTTTCTAGCAGTGAACTCAAAGTGTCACT GACGAATGTCTCAATCTCGGATGAAGGGAGATACTTCTGCCAGCTCTACACGGACCCCCCACAGGAGAGT TACACCACCATCACAGTCCTGGTTCCTCCACGTAACTTGATGATCGATATCCAGAAAGACACGGCAGTTG AAGGGGAGGAGATTGAAGTCAACTGTACTGCCATGGCCAGCAAGCCAGCGACGACCATCAGGTGGTTCAA AGGGAACAAGGAACTCAAAGGCAAATCAGAGGTGGAGGAGTGGTCGGACATGTACACTGTGACCAGTCAG CTGATGCTGAAGGTGCACAAGGAGGACGACGGGGTCCCGGTGATCTGCCAGGTGGAGCACCCTGCGGTCA CTGGAAACCTGCAGACCCAGCGCTATCTAGAAGTGCAGTATAAACCGCAAGTGCATATCCAGATGACTTA CCCTCTGCAAGGCCTAACCCGGGAAGGGGATGCATTTGAGTTAACGTGTGAAGCCATCGGGAAGCCCCAG CCTGTGATGGTAACTTGGGTGAGAGTCGATGATGAAATGCCTCAACATGCCGTACTGTCTGGGCCAAACC TGTTCATCAATAACCTAAACAAAACAGATAACGGTACTTACCGCTGTGAGGCTTCCAACATAGTGGGAAA GGCTCATTCGGACTATATGCTGTATGTATACGACACCACGGCGACGACAGAACCAGCAGTTCACGATTCT CGAGCAGGTGAAGAGGGGACCATTGGGGCAGTGGACCACGCAGTGATTGGTGGCGTCGTAGCCGTGGTGG TGTTTGCCATGCTATGCTTGCTCATCATTCTGGGCCGCTATTTTGCCAGACATAAAGGTACATACTTCAC TCATGAAGCCAAAGGAGCCGATGACGCAGCAGACGCAGACACAGCTATAATCAATGCAGAAGGAGGACAG AACAACTCCGAAGAAAAGAAAGAGTACTTCATCTAGATCAGCCTTTTTGTTCCAATGAGGTGTCCAACTG GCCTGTTTAGATGATAAAGAGACAGTGATACTGGAACTTTCGAGAAGCTCGTGTGGTTTTTTGTTTTGTT TTGTTTTTTTATGAGTGGGTGGAGAGATGCGAGACTGGGAAGGCTTGGGATTTGCAATGTACAAACAAAA ACAAAGAATGTTCTTTGAAAGTACACTCTGCTGTTTGACACCTCTTTTTAATCTGGTTTTAATTTGCTTT GGGTTTTGGGTTTTTTTGGTTTTTTGTTTTTTTCATTTATATTTCTTCCTACCAAGTCAAACTTGGGTAC TTGGATTTGGTTTCGGTAGATTGCAGAAAATTCTGTGCCTTGTTTTTCATTCGTTTGTTGTGTTTCTTCC CTCTTGCCCATTTATTTTTCCCAAAATCAAATTTGTTTTTTTCCCCCTCCCAAACCTCCCATTTTTTGGA ATTGACCTGCTGGAATTCCTAAGACTTTCTCCCTGTTGCCAGTTTCTTTTATTTGTGTTAACGGTGACTG CTTTCTGTTCCAAATTCAGTTTCATAAAAGGAAAACCAGCACAATTTAGATTTCATAGTTCAGAATTTAG TGTCTCCATGATGCATCCTTCTCTGTTGTTGTAAAGATTTGGGTGAAGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6414884 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6414884 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19710 same embryo
	EMAGE:19709 same embryo
	EMAGE:19707 same embryo
	EMAGE:19708 same embryo
	EurExpress:euxassay_006162 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source