EMAGE mouse gene expression spatial database

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EMAGE:19709

Gmcl1 germ cell-less homolog 1 (Drosophila) ( MGI:1345156)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006161. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006161_01	euxassay_006161_02	euxassay_006161_03	euxassay_006161_04

euxassay_006161_05	euxassay_006161_06	euxassay_006161_07	euxassay_006161_08	euxassay_006161_09

euxassay_006161_10	euxassay_006161_11	euxassay_006161_12	euxassay_006161_13	euxassay_006161_14

euxassay_006161_15	euxassay_006161_16	euxassay_006161_17	euxassay_006161_18	euxassay_006161_19

euxassay_006161_20	euxassay_006161_21	euxassay_006161_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19709_wholemount_strong.wlz

19709_wholemount_moderate.wlz

19709_wholemount_weak.wlz

19709_wholemount_possible.wlz

19709_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19709_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8876
Entity Detected:	Gmcl1, germ cell-less homolog 1 (Drosophila) ( MGI:1345156)
Sequence:	sense strand is shown >T8876 AGGGGGCGAGGTCTAGCGAAGCCGGTCCGCTGAGGCGCTACGGGCGGGGCTGAGGATGGAGGTGACTGCG TTCGCGGCGACGGTTGGGGCTGCGCGCGAGGCGGCAGCGGAGTAGGCGGTGGAGATACGGGCATGGCGGG GCGGCCCCTCGGTGCTCCCTAGGCGCCGGAGCCATGGGCGCTCTCAGCAGCCGGGTGCTGCGGCCCGCAG GGCGCACAGAGCAGCCCGAACCCACGCCCGGGGCTGGGGGCGCGGCCCGCAGGTCGGACGCCGGCGAAGA TGCGGGCCACAGCTTCTGTTACTGTCCGGGCGGCCGCAAGCGCAAGCGCAGCAGCGGCACATTCTGCTAC TGTCACCCCGACTCCGAGACAGACGACGACGAGGACGAGGGCGACGAGCAGCAGAGGCTGCTGAACACGC CGCGCAGAAAAAAATTAAAGAGCACATCAAAATACATCTACCAAACGCTGTTTTTGAATGGTGAAAACAG TGACATTAAGATCTGTGCTCTAGGTGAAGAGTGGAGCTTACACAAAATCTACTTATGTCAATCTGGCTAC TTTTCTAGTATGTTCAGTGGTTCTTGGAAAGAATCCAGCATGAATATTATTGAACTGGAGATTCCTGACC AGAACATTGATATAGAAGCACTGCAGGTCGCATTTGGATCACTGTATCGAGATGACGTCTTAATAAAGCC CAGCAGGGTCGTTGCCATTTTGGCAGCAGCTTGCATGCTGCAATTGGATGGTTTGATACAGCAGTGCGGT GAGACAATGAAGGAGACCATCTCTGTGAGAACTGTGTGTGGCTATTACACATCGGCAGGGACCTATGGAC TAGACTCTGTAAAGAAAAAGTGCCTCGAGTGGCTGCTGAACAACCTCATGACTCACCAGAGTGTGGAGCT TTTCAAAGAACTCAGTATAAACGTCATGAAACAGCTCATTGGTTCGTCTAACTTATTTGTGATGCAAGTG GAGATGGATGTATATACAGCTCTTAAAAAGTGGATGTTCCTTCAGCTGGTGCCTTCCTGGAATGGGTCTT TAAAGCAGCTTTTGACAGAAACAGATGTCTGGTTTTCAAAGTGGAAAAAAGACTTTGAAGGGACGACTTT CCTTGAAACTGAGCAGGGAAAACCATTTGCGCCCGTGTTCAGACATTTAAGGCTACAGTACATTATCAGT GATCTGGCTTCTGCAAGGATCATTGAGCAGGATTCTCTGGTACCTTCAGAATGGCTGGCGGCAGTGTATA AACAGCAGTGGCTGGCTATGCTACGGGCTGAACAAGACAGTGAAGTGGGGCCTCAAGAAATCAATAAAGA AGAACTTGAGGGAAACAGCATGAGGTGTGGTCGAAAGCTTGCCAAAGATGGTGAGTACTGCTGGCGCTGG ACAGGCTTCAATTTCGGCTTTGACCTCCTTGTGACTTACACCAATCGATACATCATTTTCAAACGCAATA CGCTGAACCAGCCATGTAGTGGATCTGTCAGCTTACAGCCTCGAAGGAGCATAGCATTTAGATTGCGCTT GGCTTCTTTTGACAGTAGTGGGAAACTCATATGCAGTAGAGCAACTGGCTACCAAATACTGACGCTTGAA AAAGACCAGGAGCAAGTGGTGATGAACTTGGACAGCAGACTTCTGATCTTCCCTCTGTACATCTGCTGTA ACTTCTTGTATATATCACCAGAAAAAAGAACTGAGAGTAATCGTCACCCAGAAAACCCAGGACACTGAGG CACTCATCAGTGGCCAGTTTTAACTTAATGACCTACTGCGTTCACGTCCAAGGTGACTAACAGTGACCGG CCTTATGAACTGTGGGACCCTGGAGATGTCCTCACCCTCATTACATTTCTATGCACATATGAAAAAGTTT TTTAAAACTGAGAAAGCATCTGTCAAACCATGTTAAAAGGATATCAACTCTTGCTTTAATTTAGTAGCAG TAAAAATTGCTGTAGGTAAATTTCTCATTTCTTTGCAACAAGATATAGATTTAATTTTGAGCTTGAATTT CGATCCTATCTAATGTTAGTGAGTTTACTCATCTGTAAATGTGTTCCTGTTTTGTTAAGAGAATGCTAAG GACGGAGTTTAAGTGGCCAATCATAAATGCTCTTTCAATTGGTGCCTTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6414943 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6414943 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19710 same embryo
	EMAGE:19706 same embryo
	EMAGE:19707 same embryo
	EMAGE:19708 same embryo
	EurExpress:euxassay_006161 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source