EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19719

Uba2 ubiquitin-like modifier activating enzyme 2 ( MGI:1858313)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006173. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006173_01	euxassay_006173_02	euxassay_006173_03	euxassay_006173_04

euxassay_006173_05	euxassay_006173_06	euxassay_006173_07	euxassay_006173_08	euxassay_006173_09

euxassay_006173_10	euxassay_006173_11	euxassay_006173_12	euxassay_006173_13	euxassay_006173_14

euxassay_006173_15	euxassay_006173_16	euxassay_006173_17	euxassay_006173_18	euxassay_006173_19

euxassay_006173_20	euxassay_006173_21	euxassay_006173_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19719_wholemount_strong.wlz

19719_wholemount_moderate.wlz

19719_wholemount_weak.wlz

19719_wholemount_possible.wlz

19719_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19719_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8883
Entity Detected:	Uba2, ubiquitin-like modifier activating enzyme 2 ( MGI:1858313)
Sequence:	sense strand is shown >T8883 GGTTCTCCCGCCTCCGACTGTGAGGCGGCTCGTAGACGTCCCGCCATGGCACTGTCGCGGGGGTTGCCCC GGGAGCTGGCCGAGGCGGTGTCCGGCGGCCGGGTGCTGGTGGTTGGCGCGGGTGGGATCGGCTGTGAGCT GCTCAAGAACCTGGTGCTCACTGGCTTCTCCCACATCGACCTGATTGATCTGGACACTATCGATGTCAGC AACCTCAACAGGCAGTTCTTGTTTCAAAAGAAACATGTGGGAAGATCAAAGGCTCAGGTTGCCAAAGAAA GTGTGCTCCAGTTCCACCCCCAAGCCAACATTGAGGCTCACCATGACAGCATCATGAACCCAGACTATAA TGTGGAGTTTTTTCGACAGTTTATATTGGTCATGAATGCATTAGATAACAGAGCTGCACGAAACCACGTG AATAGGATGTGTCTGGCCGCTGATGTGCCTCTCATTGAGAGCGGGACTGCTGGGTATCTCGGACAGGTGA CCACTATCAAGAAGGGTGTGACTGAGTGTTACGAATGTCACCCTAAGCCTACCCAGAGGACGTTCCCTGG CTGTACGATTCGGAACACACCTTCAGAACCTATCCATTGCATCGTATGGGCCAAGTATTTGTTCAACCAG CTGTTTGGAGAGGAAGATGCTGATCAAGAAGTGTCTCCTGACAGAGCTGACCCTGAGGCTGCTTGGGAAC CAACAGAGGCTGAAGCTAGAGCTCGAGCATCTAATGAAGACGGAGACATTAAGCGTATTTCCACTAAGGA ATGGGCTAAGTCAACTGGATATGATCCAGTTAAACTTTTTACCAAGCTTTTTAAAGATGATATTAGATAT CTGCTGACAATGGACAAACTCTGGCGCAAGAGGAAACCTCCAGTTCCACTGGACTGGGCTGAAGTGCAGA GTCAAGGAGAAGCCAATGCCGATCAACAAAATGAGCCCCAGTTGGGTTTGAAAGACCAGCAGGTTCTGGA TGTAAAGAGCTATGCGAGTCTGTTTTCAAAGAGCATCGAGACTCTGCGAGTCCATTTAGCGGAGAAGGGG GATGGAGCTGAGCTCATCTGGGATAAGGACGACCCACCTGCAATGGATTTTGTTACATCTGCTGCAAACC TCAGGATGCACATTTTCAGCATGAATATGAAGAGCAGGTTTGATATAAAATCAATGGCAGGGAATATCAT TCCTGCTATAGCTACTACTAATGCAGTGATCGCCGGCTTGATAGTGTTGGAAGGATTAAAGATTTTATCT GGAAAAATAGACCAGTGTAGAACTATTTTTTTGAATAAGCAGCCAAACCCAAGAAAGAAGCTCTTGGTGC CCTGTGCACTGGATCCTCCCAACACCAACTGTTACGTTTGTGCCAGCAAGCCAGAGGTGACTGTGCGGCT GAATGTCCACAAAGTGACTGTCCTCACCTTACAGGATAAGATAGTGAAAGAAAAGTTTGCTATGGTGGCA CCAGATGTCCAAATTGAAGATGGGAAAGGCACAATCCTGATATCTTCAGAAGAGGGAGAGACAGAAGCCA ATAACCCCAAGAAGTTGTCTGATTTTGGAATTAGAAACGGCAGCCGGCTTCAAGCAGACGACTTCCTCCA GGACTATACTTTGCTGATCAACATTCTTCATAGTGAAGATCTTGGAAAGGATGTTGAATTTGAAGTAGTT GGTGACAGCCCTGAAAAAGTGGGGCCAAAACAAGCTGAAGATGCTGCCAAAAGCATTGCCAACGGCAGCG ATGACGGTGCCCAGCCTTCCACCTCCACAGCACAAGAGCAAGATGATGTGCTCATAGTTGATTCAGACGA AGAAGGCCCTTCAAATAGCACCGACTGCAGCGGGGACGACAAGGCCCGCAAGCGGAAGCTGGAAGAGAAT GAGGCTGCCAGTACCAAGAAGTGCCGCTTGGAGCAGATGGAAGATCCAGATGACGTCATAGCGTTAGACT GAGCAGGCTCCTGCCCTTGGTCTGGTCGGAACCAAAAGTTAGATCCTTTGTGTGGCTTTGATGGTTCCGT TCAGCGTTCATTTGCTGAGACTGTTCGAGACTGTTGCTGTGTTGAAGTAGGAATAGAATTTGCAACCCTT TGAGCAAAGCTGGGTGATGAGCTGAAAGAACGCGGGCGTGCATGTTGCCTTCCCTGTAAATAGCCTTAGC TATCATTTCCTAGTGGAAAACACTGTGGGGTAGGAACGCTGCAACCTGGGTATAAATATTATGCCTTTAT TTTTGGCTAGAGAAAAGTTATTTTTAGCCTAGATCTAACCATTTTCATACTCTTACCTGAAAGATTCACA GAAGTGTCAAGTGCTATGCATTGAAACTTGTTTTTTAAACATTACCTGGCACTGTGTATATTAATGTAAA ACAATGTTAATTTACCCAGATTTTCAGTTTGTACTGCCTGGTATGTCTGTGTAAGAAGCCAATTTTTGTG TATTTGTTACAGTTTCAAGTTATTTATATTTGATGTTTTGTAAACTCAAACAACAAATATACTGTACTTA TGGACCAAATAAATGATGTCTGCATACTTGTCATCTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6415071 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6415071 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19718 same embryo
	EMAGE:19721 same embryo
	EMAGE:19720 same embryo
	EMAGE:19717 same embryo
	EMAGE:19722 same embryo
	EurExpress:euxassay_006173 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source