EMAGE mouse gene expression spatial database

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EMAGE:19721

Slc7a10 solute carrier family 7 (cationic amino acid transporter, y+ system), member 10 ( MGI:1858261)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006170. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006170_01	euxassay_006170_02	euxassay_006170_03	euxassay_006170_04

euxassay_006170_05	euxassay_006170_06	euxassay_006170_07	euxassay_006170_08	euxassay_006170_09

euxassay_006170_10	euxassay_006170_11	euxassay_006170_12	euxassay_006170_13	euxassay_006170_14

euxassay_006170_15	euxassay_006170_16	euxassay_006170_17	euxassay_006170_18	euxassay_006170_19

euxassay_006170_20	euxassay_006170_21	euxassay_006170_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19721_wholemount_strong.wlz

19721_wholemount_moderate.wlz

19721_wholemount_weak.wlz

19721_wholemount_possible.wlz

19721_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19721_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 10 11 moderate expression: see section 12

diencephalon roof plate

moderate

regional

moderate expression: see section 12

choroid invagination

strong

regional

strong expression: see section 06 07 08 09 10 14 15 16 17

telencephalon marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11

pons mantle layer

strong

single cell

strong expression: see section 05 06 07 13 14 15 16

pons ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13

spinal cord ventricular layer

strong

regional

strong expression: see section 08 09 10 11

naris

moderate

regional

moderate expression: see section 11 12 15 16 weak expression: see section 10 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8880
Entity Detected:	Slc7a10, solute carrier family 7 (cationic amino acid transporter, y+ system), member 10 ( MGI:1858261)
Sequence:	sense strand is shown >T8880 GGCTGCGGGCCGGACAGACGGGGTAGCGCAGGGAACTGGGATGAGGCGGGACAGCGACATGGCAAGCCAC ATACAACAGCCAGGCGGGCACGGGAACCCCGGCCCTGCGCCCTCGCCTTCCCCGGGCCCTGGTCCCGGCC CGGGCGCCTCGGAGCGGGTGGCACTCAAGAAAGAGATCGGGCTGGTGAGCGCTTGCACCATCATCATCGG GAACATCATTGGCTCAGGCATCTTCATCTCACCCAAGGGTGTCCTGGAACACTCGGGCTCCGTGGGTTTG GCCCTCTTCGTCTGGGTCCTGGGTGGGGGCGTGACAGCTCTGGGCTCTCTCTGCTATGCAGAGCTGGGTG TCGCCATCCCCAAGTCTGGTGGGGACTACGCCTATGTCACTGAGATCTTCGGGGGCCTGGCTGGATTCCT ACTGCTCTGGAGTGCTGTCCTCATCATGTACCCCACCAGCCTGGCTGTCATCTCCATGACCTTCTCCAAC TATGTGCTTCAGCCTGTCTTTCCCAACTGTATCCCCCCAGCCACAGCCTCTCGAGTACTCTCCATGGCCT GCCTGATGCTCCTGACGTGGGTGAACAGCTCCAGCGTACGCTGGGCCACGCGCATCCAGGTTATCTTCAC TGGTGGGAAGCTGCTGGCGCTGTCTCTCATCATCACTGTTGGCTTTGTCCAGATCTTCCAAGGACACTTT GAAGAGCTGAGACCCACCAATGCCTTCGCCTTCTGGATGACACCGTCTGTGGGTCACCTGGCCCTGGCTT TCCTCCAAGGTTCTTTTGCCTTCAGTGGCTGGAACTTCCTCAACTATGTCACGGAGGAGCTGGTTGACCC ACGCAAGAACCTACCTCGTGCCATCTTCATTTCCATCCCACTGGTCACCTTTGTGTACACATTCACCAAT GTCGCCTACTTCACTGCCATGTCCCCCCAGGAGTTGCTGTCCTCCAACGCCGTGGCGGTGACCTTCGGCG AGAAGCTGCTGGGCTACTTTTCGTGGGTCATGCCCGTCTCTGTGGCCCTCTCTACTTTTGGAGGGATCAA TGGCTACCTGTTCACCTCATCCAGGCTATGCTTCTCTGGAGCCCGAGAGGGACACTTACCCAGCTTCCTG GCCATGATTCATGTCAGACGCTGCACCCCAATCCCTGCCCTCCTTGTCTGTTGCGGGGCCACAGCGGTCA TCATGCTCGTGGGTGACACATACACACTCATCAACTATGTGTCCTTCATCAACTACCTCTGCTACGGAGT CACTATCCTGGGCCTGCTTGTGCTGCGCTGGAGACGGCCGGCACTCCACAGGCCCATTAAGGTGAACCTC CTCGTTCCTGTTGTGTACTTGGTGTTCTGGGCATTCCTACTGGTCTTCAGCTTCATCTCGGAGCCCATGG TCTGTGGGGTCGGCATCATCATTATCCTCACTGGGGTTCCCATCTTCTTCCTGGGAGTGTTCTGGAGAAG CAAACCAAAGTGTGTACACAGATTCACAGAGTCCATGACACGCTGGGGCCAGGAGCTGTGTTTCGTGGTT TACCCCCAGGGCTCCCTAGAGGAGGAGGAAAATGGCCCCATGGGCCAGCCCTCCCCATTGCCCATCACGG ACAAGCCCTTGAAGACACAATGAGACCTTGTAGAGACTGGAACAGCCGATTCTGTTTACATGTTGTTTAT TGAGAAGGGGGTTGTGTTTTGTTTTGTTTTCAAAAATTTTTTTTCTGCAAAAAAAGAGAGAGAGAGAGAG AGAGAGAGAGAGAAAGAGAGACCCAACCCTTAGAGGCCTATTGTAAGGGACTGGCCACTGGGGTCGGGCT TCCTGCCTTAGCAATGCCCTGCTAGCTTCTCCAGAAATGCCTGTAAATAAACAGGGCTGGTTGTTGCTGT GTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6414759 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6414759 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19718 same embryo
	EMAGE:19720 same embryo
	EMAGE:19719 same embryo
	EMAGE:19717 same embryo
	EMAGE:19722 same embryo
	EurExpress:euxassay_006170 same experiment
	MGI:4828272 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source