EMAGE mouse gene expression spatial database

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EMAGE:19723

Zc3h3 zinc finger CCCH type containing 3 ( MGI:2663721)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006202. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006202_01	euxassay_006202_02	euxassay_006202_03	euxassay_006202_04

euxassay_006202_05	euxassay_006202_06	euxassay_006202_07	euxassay_006202_08	euxassay_006202_09

euxassay_006202_10	euxassay_006202_11	euxassay_006202_12	euxassay_006202_13	euxassay_006202_14

euxassay_006202_15	euxassay_006202_16	euxassay_006202_17	euxassay_006202_18	euxassay_006202_19

euxassay_006202_20	euxassay_006202_21	euxassay_006202_22	euxassay_006202_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19723_wholemount_strong.wlz

19723_wholemount_moderate.wlz

19723_wholemount_weak.wlz

19723_wholemount_possible.wlz

19723_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19723_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8903
Entity Detected:	Zc3h3, zinc finger CCCH type containing 3 ( MGI:2663721)
Sequence:	sense strand is shown >T8903 CGGACTCCGGAGATGGAGGAAAAGGAGCAGCTGCGGCGGCAGATACGCCTCCTGCAGGGTCTAATTGATG ACTATAAAACACTCCACGGCAATGGCCCTGCCCTGGGCAACTCATCAGCTACTCGGTGGCAGCCACCCAT GTTCCCGGGTGGCAGGACCTTTGGCGCCCGCTACTCCCGTCCAAGTCGGAGGGGCTTCTCCTCACACCAT GGCCCTTCGTGGCGCAAGAAATACTCCCTTGTGAATCAGCCTGTGGAATCTTCTGACCCAGCCAGCGATC CTGCTTTTCAGACATCCCTCAGGTCTGAGGATAGCCAGCATCCTGAACCCCAGCAGTATGTACTGGAGAG ACAGGTCCAGCTCAGTCCAGATCAGAATATGGTTATTAAGATCAAGCCACCATCAAAGTCAGGTGCCATC AATGCTTCAGGGGTCCAGCGGGGGTCCTTGGAAGGCTGTGATGACCCCTCTTGGAGTGGCCAAAGACCCC AAGGAAGTGAGGTTGAGGTCCCTGGTGGACAACTGCAGCCTGCAAGGCCAGGAAGAACCAAGGTGGGTTA CAGTGTGGACGACCCCCTCTTGGTCTGCCAGAAGGAGCCTGGCAAGCCTCGGGTAGTGAAGTCTGTGGGC AGGGTGAGTGACAGCTCTCCCGAGCATCGGCGGACAGTCAGTGAAAATGAAGTGGCCCTCAGGGTACACT TCCCATCTGTCCTGCCCCATCACACTGCTGTGGCTCTGGGCAGGAAGGTAGGCCCTCATTCTACCAGCTA TTCTGAACAGTTCATTGGAGACCAAAGAGCAAACACTGGCCACTCAGACCAGCCAGCTTCCTTGGGGCCA GTGGTGGCTTCAGTCAGACCAGCAACAGCCAGGCAGGTCAGGGAGGCCTCACTGCTCGTGTCCTGTCGAA CCAGCAAGTTTCGGAAAAACAACTACAAATGGGTAGCTGCCTCAGAAAAGAGCCCACGGGTCGCTCGGAG AGCCCTCAGTCCCAGAACAACTCTGGAGAGCGGGAACAAGGCCACTTTGGGTACAGTTGGAAAGACAGAG AAGCCACAGCCTAAAGTTGACCCAGAGGTGAGGCCGGAGAAACTGGCCACACCATCCAAGCCTGGCCTCT CTCCCAGCAAGTACAAGTGGAAGGCTTCCAGCCCGTCTGCTTCCTCCTCTTCCTCTTTCCGTTGGCAGTC TGAGGCTGGCAGCAAGGACCATACTTCTCAGCTCTCCCCAGTCCCATCTAGGCCCACATCAGGGGACAGA CCAGCAGGGGGACCCAGCAGCTTGAAGCCCCTCTTTGGAGAGTCACAGCTCTCAGCTTACAAAGTGAAGA GCCGGACCAAGATTATCCGGAGGCGGGGCAATACCAGCATTCCTGGGGACAAGAAGAACAGCCCTACAAC TGCCACCACCAGCAAAAACCATCTTACCCAGCGACGGAGACAGGCCCTCCGGGGGAAGAATAGCCCGGTT CTAAGGAAGACTCCCCACAAGGGTCTGATGCAGGTCAACAGGCACCGGCTCTGCTGCCTGCCGTCCAGCC GGACCCACCTCTCCACCAAGGAAGCTTCCAGTGTGCACATGGGGATTCCACCCTCCAATAAGGTGATCAA GACCCGCTACCGCATTGTTAAGAAGACCCCAAGCTCTTCCTTTGGTGCTCCATCCTTCCCCTCATCTCTA CCCTCCTGGCGGGCCCGGCGCATCCCATTATCCAGGTCCCTAGTGCTAAACCGCCTTCGTCCAGCAATCA CTGGGGGAGGGAAAGCCCCACCTGGTACCCCTCGATGGCGCAACAAAGGCTACCGCTGCATTGGAGGGGT TCTGTACAAGGTGTCTGCCAACAAGCTCTCCAAAACTTCTAGCAGGCCCAGTGATGGCAACAGGACCCTC CTCCGCACAGGACGCCTGGACCCTGCTACCACCTGCAGTCGTTCCTTGGCCAGCCGGGCCATCCAGCGGA GCCTGGCTATCATCCGGCAGGCGAAGCAGAAGAAAGAGAAGAAGAGAGAGTACTGCATGTACTACAACCG CTTTGGCAGGTGTAACCGTGGCGAATGCTGCCCCTACATCCATGACCCTGAGAAGGTGGCCGTGTGCACC AGATTTGTCCGAGGCACATGCAAGAAGACAGATGGGTCCTGCCCTTTCTCTCACCATGTGTCCAAGGAAA AGATGCCTGTGTGCTCCTACTTTCTGAAGGGGATCTGCAGCAACAGCAACTGCCCCTACAGCCATGTGTA CGTGTCCCGCAAGGCTGAAGTCTGCAGTGACTTCCTCAAAGGCTACTGCCCATTGGGTGCAAAGTGCAAG AAGAAGCACACGCTGCTGTGTCCTGACTTTGCCCGCAGGGGTATTTGTCCCCGTGGCTCCCAGTGCCAGC TGCTCCATCGTAACCAGAAGCGACATGGCCGGCGGACAGCTGCACCTCCTATCCCTGGGCCCAGTGATGG AGCCCCCAGAAGCAAGGCCTCAGCTGGCCACGTACTCAGGAAGCCTACTACTACTCAGCGCTCTGTCAGA CAGATGTCCAGTGGTCTGGCTTCCGGAGCTGAGGCCCCAGCCTCCCCACCTCCCTCCCCAAGGGTATTAG CCTCCACCTCTACCCTGTCTTCAAAGGCCACCGCTGCCTCCTCTCCTTCCCCCTCTCCCTCTACTAGCTC CCCAGCCCCTTCCTTGGAGCAGGAAGAAGCTGTCTCTGGGACAGGCTCAGGAACAGGCTCCAGTGGCCTC TGCAAGCTGCCATCCTTCATCTCCCTGCACTCCTCCCCAAGCCCAGGAGGACAGACTGAGACTGGGCCCC AGGCCCCCAGGAGCCCTCGCACCAAGGACTCAGGGAAGCCGCTACACATCAAACCACGCCTGTGAGGCCC CCTGAGGACCAGCCCGCACCTACCTCAGACCCTCACCCCTGGAGAGGATGAAGGCTCTACCCACAACTGC TCCTGAGGAGAAGCAGCCTGCCACTGAGCCCAGCTGGGGCTCTAACCAGGAGTCATAGCTCCTCCCACTT GGCTCCTAGCCTCTGGAGGCCTTTGTGTGCCCAGGCCTTGTTCTCCCTCAAGCCAAGGCCCTGTCCATTC CCTGTTCCTCTCCCTCCCCTTCTCCCCTCCTCCCCTCTGCCCCTCCTCCCCTCCAAGGCACCCAGCCTCA CTCTAGGACTTGTCCCTGGCTCCCCATCCATCAGGGTGCCATGTGAGGTTCATCCCAGCCTGTCTCCTGT GGTGGCATTGGCAGCTGTAGGACTGGTGCCCCCAACCCTCCCTACCCAGGACTCCTGCCCAGGGAAGAGG CTAATGGCCAGGCCTCTCTGGTGGGTATTTTATGTTCAGCAATAAAGGTTTTATCCTGTGAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6416246 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6416246 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19725 same embryo
	EMAGE:19728 same embryo
	EMAGE:19724 same embryo
	EMAGE:19726 same embryo
	EMAGE:19727 same embryo
	EurExpress:euxassay_006202 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source