EMAGE mouse gene expression spatial database

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EMAGE:19745

Atp6v1g1 ATPase, H+ transporting, lysosomal V1 subunit G1 ( MGI:1913540)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006208. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006208_01	euxassay_006208_02	euxassay_006208_03	euxassay_006208_04

euxassay_006208_05	euxassay_006208_06	euxassay_006208_07	euxassay_006208_08	euxassay_006208_09

euxassay_006208_10	euxassay_006208_11	euxassay_006208_12	euxassay_006208_13	euxassay_006208_14

euxassay_006208_15	euxassay_006208_16	euxassay_006208_17	euxassay_006208_18	euxassay_006208_19

euxassay_006208_20	euxassay_006208_21	euxassay_006208_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19745_wholemount_strong.wlz

19745_wholemount_moderate.wlz

19745_wholemount_weak.wlz

19745_wholemount_possible.wlz

19745_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19745_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 08 09 10 11 12

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 07 08 15 16 17 18

vibrissa

moderate

regional

moderate expression: see section 07 08 09 10 21 22

facial vii ganglion

moderate

regional

moderate expression: see section 02 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 05 06 14

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05

dorsal root ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 10 11 12 13

not examined

regional

not examined expression: see section 01 02 03 04

neural retina

strong

regional

strong expression: see section 01 02 03 04

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T699
Entity Detected:	Atp6v1g1, ATPase, H+ transporting, lysosomal V1 subunit G1 ( MGI:1913540)
Sequence:	sense strand is shown >T699 TCCTCGAGNCTGTTGGCCTACTGGGACGGTGGGGCGGGGCTCCTGCGCGCTGCGCAGGCGCAGAGTCTGG GTGGTGGATCAGGTGATCCGAGGGGCTTCGGAGGGTGTTTGAGCAGTTGACTTAGGCCGTTCTCTCAGCC CAAAGTCCCCTCAGCAATGGCGAGTCAGTCGCAGGGCATCCAGCAGCTACTGCAGGCCGAGAAGCGGGCC GCCGAGAAGGTGTCCGAGGCCCGCAAGCGAAAGAACCGGAGGCTGAAGCAGGCCAAAGAAGAAGCCCAGG CTGAAATTGAACAGTACCGCCTGCAGAGGGAGAAGGAGTTCAAGGCCAAGGAAGCTGCGGCATTGGGCTC CCATGGCAGCTGCAGCAGTGAGGTGGAAAAGGAGACCCGGGAGAAGATGACCGTCCTCCAGAACTACTTC GAGCAGAACAGGGATGAAGTCCTGGATAACCTCTTGGCCTTTGTGTGTGACATCCGGCCGGAAATCCATG AAAACTACCGCATAAACGGATAGGAGGTGGAAGCGCCTGCTCTGTGGGTTGGCATGAGATCTGCCCCCAT AGA
Notes:	The probe template was PCR amplified from IMAGE:1889247 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1889247 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19748 same embryo
	EMAGE:19747 same embryo
	EMAGE:19744 same embryo
	EMAGE:19746 same embryo
	EurExpress:euxassay_006208 same experiment
	MGI:4823336 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source