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EMAGE:19748

Fbxw5 F-box and WD-40 domain protein 5 ( MGI:1354731)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19748 EMAGE:19748 EMAGE:19748 EMAGE:19748 EMAGE:19748
"Pseudo-wholemount" of euxassay_006218. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006218_01 euxassay_006218_02 euxassay_006218_03 euxassay_006218_04
EMAGE:19748 EMAGE:19748 EMAGE:19748 EMAGE:19748 EMAGE:19748
euxassay_006218_05 euxassay_006218_06 euxassay_006218_07 euxassay_006218_08 euxassay_006218_09
EMAGE:19748 EMAGE:19748 EMAGE:19748 EMAGE:19748 EMAGE:19748
euxassay_006218_10 euxassay_006218_11 euxassay_006218_12 euxassay_006218_13 euxassay_006218_14
EMAGE:19748 EMAGE:19748 EMAGE:19748 EMAGE:19748 EMAGE:19748
euxassay_006218_15 euxassay_006218_16 euxassay_006218_17 euxassay_006218_18 euxassay_006218_19
EMAGE:19748 EMAGE:19748 EMAGE:19748
euxassay_006218_20 euxassay_006218_21 euxassay_006218_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19748Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19748_wholemount_strong.wlz
19748_wholemount_moderate.wlz
19748_wholemount_weak.wlz
19748_wholemount_possible.wlz
19748_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19748_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T574
Entity Detected:Fbxw5, F-box and WD-40 domain protein 5 ( MGI:1354731)
Sequence:sense strand is shown

>T574
TCTCGAGNCTGTTGGCCTACTGGAGACTAGAGCGGCGGCGGAAGCCGGGCGCAGCGCAGCGGGCAGAGGC
ACCGCGGACCGTCTGGGCAGAATGTCACGATGGATGAGGGGGGCCTGCCCCTGCTCCCCGACAGCCTTGT
CTACCAGATCTTCCTGAGTTTGGGCCCTGCAGATGTGCTGGCTGCTGGGCTGGTATGCCGCCAATGGCAG
GCTGTGTCCCGGGATGAGTTCTTATGGAAGGAGCAATTCTACCGCTACTACCAGGTGGCTCGAGATGTGC
CCCGCCACCCAGCGGCCACATCCTGGTATGAGGAGTTCCGGCGGCTCTATGACATGGTACCCTGTGTGGA
GGTGCAGACACTGAAGGAGCACACTGACCAGGTCCTGCACCTCAGCTTCTCCCACTCGGGGTACCAGTTT
GCCTCCTGCTCCAAGGACTGCACGGTGAAGATCTGGAACAACGACCTGACCATCTCACTGCTGCACAGTG
CAGATATGAGGCCATACAACTGGAGTTACACCCAGTTTTCCCAGTTCAACCAGGATGACTCACTGCT
Notes:The probe template was PCR amplified from IMAGE:1885023 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1885023 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19745 same embryo
 EMAGE:19747 same embryo
 EMAGE:19744 same embryo
 EMAGE:19746 same embryo
 EurExpress:euxassay_006218 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS