EMAGE mouse gene expression spatial database

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EMAGE:19784

Spice1 spindle and centriole associated protein 1 ( MGI:1196252)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006294. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006294_01	euxassay_006294_02	euxassay_006294_03	euxassay_006294_04

euxassay_006294_05	euxassay_006294_06	euxassay_006294_07	euxassay_006294_08	euxassay_006294_09

euxassay_006294_10	euxassay_006294_11	euxassay_006294_12	euxassay_006294_13	euxassay_006294_14

euxassay_006294_15	euxassay_006294_16	euxassay_006294_17	euxassay_006294_18	euxassay_006294_19

euxassay_006294_20	euxassay_006294_21	euxassay_006294_22	euxassay_006294_23	euxassay_006294_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19784_wholemount_strong.wlz

19784_wholemount_moderate.wlz

19784_wholemount_weak.wlz

19784_wholemount_possible.wlz

19784_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19784_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15

adenohypophysis

moderate

regional

moderate expression: see section 11 12 13 14 15 16

not examined

regional

not examined expression: see section 13

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15 16

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 12 13 18 19

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 13 weak expression: see section 08 09 10

rest of cerebellum ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 17 18 19 20 21 22 weak expression: see section 06 07 08 09 10 15 16

pons ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 weak expression: see section 08 09 10 13

midbrain ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 weak expression: see section 06 07 08 09 10 13 14 15 16

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 16 17 18 19 20 21

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 15 18

testis

moderate

regional

moderate expression: see section 05 06 07 08 09 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8470
Entity Detected:	Spice1, spindle and centriole associated protein 1 ( MGI:1196252)
Sequence:	sense strand is shown >T8470 GTTACCATTCTGGAGGCTGAACTTTTAGTCGGGGCGGAGGCACAGTCGGCCAGCGGCAAGATGTCATTGA TCAGAGTGAACCGCTTTGGTCCCCGGGGAGGTGGAAGAAAAACTCTGAAAGTAAAGAAGAAAGCTGCAGT GAGGCAAGAATGGGATAATACTGTGAATGATCTAACAGTTCACCGGGCAACTCCTGAAGATCTAGTGCGC CGTCATGAGATGCACAAGTCAAAGAATAGAGCATTGGTGCACTGGGAACTTCAAGAAAAAGCTTTGAAGA GAAAATGGAAGAAGCAGAAACCAGAAACTTCAAGTCTTGAAAAAAGAAAGTTATCTATCATGAAGGAGAT TCTCTCTGATCAATACCTGACACAGGATGTGCTGGAAAAGTCCGATCATCTGATGGCTGCAGCCAAAGGT CTGTTTGCTGATGTTCCTCGTAAGCGCACAGGGTTTCCAAATGTAACAAGGGCTCCAGATTCCTCACAGA GCCACACTGGAATAAATCAAGACCCTGTCACCCAGTCTATCCTTAATGAATCAATCATAGAGCCTCAGGC TCTTAATGAAGTCGACGATGCAGGGGAGCAGAGCACTGCTCACAGCCAGTCAGAAGACAGCGAGAATGAA TTGCCTAACTCCCTCAGCCAGCATTCTAACAGGAGTACAGAGAGGTTTCTTCACCAACTAAAGGAAGAGA ATTCCGAGTTAATCAATCAACTGTGGACTGATATTCAGCAGAAAATCGCTACCCAGTCACAAAGAACTCC TCCAGGATCTCCGTCCTCGGAGCTTTCAGCCGAGGATCAGAAAGCTGCTCTGAATGCAACGGATGCTGTC AAGAGAATCCAGGCTGGGCCTCAGCCTGAGGAGGCTGCTGAGCCTGTAGACTTCAGCTCCAGCTACCTGG GACAGGTGCTAAACACAAGGAAGCAAAAGCCGCTGCTAGCTAAAGTGAAAAGAAAACAAGATATGCATGC TGCCTCCAAGCAGAAGACAAACATGCTGTCCTCCAGCACAGCCTCTGCAGACAGACCCAGTAGCACTGGC TCCAGCCTGGACGTTCTCAAACACGTGATCCATGAAGTGGAGCACGAGATGGAAGAGTATGAGCGGTGCA CAGGGCGTGAGGTCACGGGGCTGCAGGGTGGTCAGGGACTCACAGGCTTCACCCTGTCCCTGGTGAGCTC TCTCTGCCGCCTGGTTCGGTACCTTAAAGAGAGTGAAATCCAACTTCGGAAAGAAGTAGAGACAAGGCAG CAGCTGGAACAGATGTTAGGTGATCACCGAGAGCTCATTGATGCCCTGACAGCTGAAATCCTCTCCCTCA GAGAAGAGAATAGTACCATGCAGGCGAGACTTCAGCAGTACATGGTTACCACAGATGAGCAGCTCATATC GCTCACACATGCCATTAAGAACTGTCCTGTGATAAATAACTCCAGACAAGAAAGTCAAGCACCAGAAAGG GCAGCTATGGGTAGAAGACTTGTGGATAATGTAGAGGGTCCTGTTATAAGCAGTAATGGCTCCATGCCAT TGATGTTCAGAGGGGAAGAAGTGGTTGAATTCCCACAGGAAGAGTTGCCTGTTAAACTATCTCAGGGGCC AACCCCCACAGAAAACTTGAATCTGGCCAATAATTTCCCAACCCATATTTTCGAGCCAGCTGTGATGCTA ACACCACCCAGGCAGAAGAGCAACTCAGAATTCTCTCCTCTCCAGGATGTTCTGAGGAGGACTGTTCAGA CTCGTCCGGCCCCACGAATTCCCCCCACTGTGGAAGTTATAGAGAAGGAGCAGAACTGGGAAAAGAAGGC CCTGCCTATTGATCCAGACATTCAGAACTCAAGTGAGGAAAACCGTCTCTTCACTCAGAGGTGGAGAGTC TCGCACATGGGAGAAGACCTGGAGAACAAAGGGCAGCCAGCATTTGTTAGCCTGTCACAGCCGCCTTGTA GTTCCCTTCCCAGCACTCAGCAGCCAAGAAACCCCGTGTTGTCAGAGGAGCCCACTGTGCTTGGAGATGG GCAGCAGCTCAGAACATCGGAGGCCCTGGTGCAGAGGAAGGACATTATGGCCCGGATCGCCGAGCTCACA CTGCAGAATTCAGCCATCAAGGCCCACCTGAACAATATTACCAGCTCAGGGGGAGAGCAAGGTGATGGAC TGCGGGAGCCACGCAAACAGGGAAGTGCCAGTGAAGTGTCTACTAATTTCCCAGCAGTTCAGTCTTTAAC GCCAAGTAGCATGGAGGAGCGGATTGCAGAGTTGAACCGACAGAGCATGGAGGCGCGCAGCAAACTGCTC CAGCTGATAGAGCAGCAGAAACTTGTGGGCTTGAACCTTTCCTCATCACCGGTGTCACCCGTGGAGTCAC CTCTCAGAGCGTGGGCCGAAGAAGGAAAGCGAACCATCGAGGTGTCTGTTCCAGGAATGGAGGCTTCAGA GAGTTCAAAATGCAATACTGTGTCTCCTGTCAGTGGGAACAGTTCAAGAAGATCGTCAGGGGCGATCAGC AATTCTTGTTCTCCACTAAACGCCACCTCAGGAAGTGGGAAGTTCACACCAGTTAATCCAAGAACAAAGA CTGAAAAAAAAAATGAAGAAGGTTGGTTTGCTCTCTCTGCCCACATACCGTAAGTGAAGCCCCAGCAGAG AGTGTCCTCAGTGACTTATTCCTGGCCTTTCCTTCGCACTTCACAGATTCCATTTCAAGTTAATAACATT TTTCTTCAGATAAAACCTGCATAGGTATCCTGTATTTATGGAAACAGAAAAAAGAATTATTTTAATATTT TACCTTTTCAAATGTTTTTTCAACAAAGCACCTCTAATTCCCTGTGAGTAAAGCTTTGCAGGAGTTTAAG TTTTATATTTGTTTAGCCTTGTTAAATGCATTAGTGATACATTGTTACTTTTGTCATTTTGGTTGAATGT TTGTTTGTTATTTAGTGCATCTTAAATGCAATCACCTTCCCACAAGAACTAATTGGCTTTATGTATAACT TAAAATAAAGTTGTTCTGGCTTTCAGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5703283 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5703283 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19786 same embryo
	EMAGE:19785 same embryo
	EMAGE:19788 same embryo
	EMAGE:19789 same embryo
	EMAGE:19787 same embryo
	EurExpress:euxassay_006294 same experiment
	MGI:4823679 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source