EMAGE mouse gene expression spatial database

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EMAGE:19789

Oprl1 opioid receptor-like 1 ( MGI:97440)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006301. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006301_01	euxassay_006301_02	euxassay_006301_03	euxassay_006301_04

euxassay_006301_05	euxassay_006301_06	euxassay_006301_07	euxassay_006301_08	euxassay_006301_09

euxassay_006301_10	euxassay_006301_11	euxassay_006301_12	euxassay_006301_13	euxassay_006301_14

euxassay_006301_15	euxassay_006301_16	euxassay_006301_17	euxassay_006301_18	euxassay_006301_19

euxassay_006301_20	euxassay_006301_21	euxassay_006301_22	euxassay_006301_23	euxassay_006301_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19789_wholemount_strong.wlz

19789_wholemount_moderate.wlz

19789_wholemount_weak.wlz

19789_wholemount_possible.wlz

19789_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19789_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

diencephalon lateral wall marginal layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

lateral ventricle

strong

regional

strong expression: see section 04 05 06

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon marginal layer

strong

regional

strong expression: see section 01 02 03 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

medulla oblongata basal plate marginal layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

rest of cerebellum mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

rest of cerebellum marginal layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

pons mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

pons marginal layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain marginal layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

facial vii ganglion

strong

homogeneous

strong expression: see section 04 05 06 07 08 19 20 21 22

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 06 07 18 19

trigeminal v ganglion

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vagus x ganglion

strong

homogeneous

strong expression: see section 07 18

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 06 07 08 18 19 20

trigeminal v nerve

strong

homogeneous

strong expression: see section 10 11 18 19

spinal cord mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 14 15 16

spinal cord marginal layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

spinal cord sulcus limitans

strong

regional

strong expression: see section 13

peripheral nervous system

strong

regional

strong expression: see section 16

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 10 14 15 16

cervical ganglion

strong

regional

strong expression: see section 08 09 17

thoracic ganglion

strong

regional

strong expression: see section 11 12 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 15 16 17 18

neural retina

strong

regional

strong expression: see section 02 03 04 05 moderate expression: see section 24

atrioventricular canal

moderate

regional

moderate expression: see section 13 14 15

tongue

moderate

spotted

moderate expression: see section 14 16

stomach

moderate

regional

moderate expression: see section 06 07 08 09 weak expression: see section 04 05

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8471
Entity Detected:	Oprl1, opioid receptor-like 1 ( MGI:97440)
Sequence:	sense strand is shown >T8471 GCCATTCCCAGCCGGAGCAGACCCCAAGCTAGAGTGAGAAGCATTACTCAGTTCATTGTGCTCCTGCCTG CCTTTCTGCTAAGCATTAGGGTCTGTTTTGGCCCAGCTTCTGAAGAGGTTGTGTGTGCTGTTGGAGGAAC TGTACTGAGTGGCTTTGCAGGGTGACAGCATGGAGTCCCTCTTTCCTGCCCCATTCTGGGAGGTCTTGTA TGGCAGCCACTTTCAAGGGAACCTGTCTCTCCTAAATGAGACCGTACCCCATCACCTGCTCCTCAATGCT AGCCACAGTGCCTTCCTGCCCCTTGGACTCAAGGTCACCATCGTGGGGCTCTACTTGGCTGTGTGCATCG GGGGGCTCCTGGGGAACTGCCTCGTCATGTATGTCATCCTCAGGCACACCAAGATGAAGACTGCTACCAA CATTTACATATTTAATCTGGCACTGGCTGATACCCTGGTCTTGCTGACACTGCCCTTCCAGGGCACAGAC ATCCTTCTGGGCTTCTGGCCATTTGGGAATGCACTGTGCAAGACGGTCATTGCTATCGACTACTACAACA TGTTTACCAGCACTTTCACTTTGACTGCCATGAGTGTAGACCGTTATGTAGCTATCTGCCACCCTATCCG TGCCCTTGATGTTCGGACATCCAGTAAAGCCCAGGCCGTTAATGTGGCCATATGGGCCCTGGCTTCGGTG GTTGGTGTTCCTGTTGCCATCATGGGCTCAGCACAAGTGGAGGATGAAGAGATCGAGTGCCTGGTGGAGA TCCCCGCCCCTCAGGACTATTGGGGCCCTGTATTTGCCATCTGCATCTTCCTTTTTTCCTTCATCATCCC GGTTCTGATCATCTCTGTCTGCTACAGCCTCATGATTCGACGACTTCGTGGTGTCCGGCTGCTTTCAGGC TCCCGAGAGAAGGACCGGAACCTGCGACGCATCACACGGCTGGTACTGGTAGTTGTGGCTGTGTTTGTGG GCTGCTGGACACCTGTGCAGGTCTTTGTCCTGGTTCAAGGACTGGGTGTTCAGCCAGGTAGTGAGACTGC AGTAGCCATTCTGCGCTTCTGCACAGCCCTGGGCTATGTCAACAGTTGTCTCAATCCCATTCTCTATGCT TTCTTGGATGAGAACTTCAAGGCCTGCTTTAGAAAGTTCTGCTGTGCTTCTGCCCTGCACCGGGAGATGC AGGTTTCTGATCGTGTGCGCAGCATTGCCAAGGATGTAGGCCTTGGTTGCAAGACCTCTGAGACAGTACC ACGGCCGGCATGACTAGGCGTGGACCTGCCCATGGTGCCTGTCAGTCCACAGAGCCCATCTACACCCAAC ACGGAGCTCACACAGGTCACTGCTCTCTAGGTTGACCCTGAACTGAGCGTCTGGGGCCTTGAATGGCTTT TCTTTTGGTTCAGGATGCTCAGTCCTAGAGGAAGACCTTTTAGCACCATGGGACAGGTCAAAGCATCAAG GTGGCCTCCATGGCCTCTGTCAGATTAAGTTTCCTCCCTGGTATAGGACCAGAGAGAACCAAAGGAACTG CATGGAAACATCCACAACTCAGTGGACATGCCTGGTGAACCCATGTAGGTATTCATGGTTCACTTGACTC TTCTCTGGTTTCTCCCTGCTGCCCTGGTTCTAGGTGGGCTCAGCCTGAGGTATTGTAGTTGTCATGTAGT CACTATTGTGACTACCTGTTGTGTGCTATTGCCCTCAGCCTTTCAGTGTTTGCACAGAACTGGTGATCAT ACCCAGTGTTGCCTGGCCCTTAAGCTTGGAGTTGCCTTGGAGCATCTAGTTCTGACTCCACTGATGCATT CAGATTACCTGAGGTGGGTGAGCATCAGTGGGTTCTTGGATGACTGTTTCCTGACGATTCTTTTCATGCT GTACTATGGAGTATATGAAGGGGACTTCACACTTCATCTGGTACTGCCACTGCCTGCTCTACCAACCTGG ACCACCTTCTCAGCAAGAGGCTAGCAGGGGGACAAGACACAAAGCTTCCCTAAGGCTCTTTCCCTCCAAA ACCACTGTGAACTCTTATTCTACAGACTGTTTGGCAAGCCCTGCTTCTAACTGTGTGGGAAGTAATCAGG AGAAAATTCTGTGGCCTCTGTAGGCTGCTCACAGCATGGAGGCACCACATGCTGGTCTTGGGTATGTGTC TTGGCTGCTCAGTATGGGCAGGGCAGGGCACGAGACTATGCTCTCTCCTTATTCTCCACAGCCTCCCTCA GCTCTCCAGCAGTCGCTCTTTTACTTGACAGTAGAGGTCAGCAGTTGTACTGCTAGAAACACACTTGTAG CCCGGGAAGACTGGAGTCAGGATGTGTTCTATTCTATACCCACAGTGACCACCTGCTTCATTTATAGGGT TAGGACATATCCAAGCAAGGCCTGGGCTTGGCATCAAATGAAGAGCTGGTATGAGAGCTGAAGCCTAAAA TGGCTCATTTGAGCCAATCTGCAAGGACTATTACGGTTTTGGGGACATTGGAAGAAGAGTCGATACCTTG GAGATATATTGTTGGTTCACAGAAGAAGAGGCTTTGTAAATGCCCTTTCTATGGGTCAGATAAAAAAATA CCAGCAACCTTGGATAGAATCTGATCTTACACAGAGACCTTGGTTAGGTGGTTTCATGCCATGCAGAACC CAGGCTGGTTCCTGTCTCAGAGAGACCTGGCCTTAACAATCTTTCTGGGGAGACCAGGGTTCTTTCAATG ACACTTGTGCAGGAGCCATTAGCTCTAGTGTTTTCTTGCTAATGCTAGAAACTTTGTGAATTTGTATATC TTTATGTATTTAAGCATGCAACAAATGTCATTTTCGTGCATGTAGCTAGCCCTGAACCTGCCTCTGGGGT AATGAAGGAGTTCTCATAATAAACACTTACCAGTGACCAGTAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5703258 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5703258 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19786 same embryo
	EMAGE:19785 same embryo
	EMAGE:19784 same embryo
	EMAGE:19788 same embryo
	EMAGE:19787 same embryo
	EurExpress:euxassay_006301 same experiment
	MGI:4826978 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source