EMAGE mouse gene expression spatial database

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EMAGE:19812

Arnt2 aryl hydrocarbon receptor nuclear translocator 2 ( MGI:107188)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006289. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006289_01	euxassay_006289_02	euxassay_006289_03	euxassay_006289_04

euxassay_006289_05	euxassay_006289_06	euxassay_006289_07	euxassay_006289_08	euxassay_006289_09

euxassay_006289_10	euxassay_006289_11	euxassay_006289_12	euxassay_006289_13	euxassay_006289_14

euxassay_006289_15	euxassay_006289_16	euxassay_006289_17	euxassay_006289_18	euxassay_006289_19

euxassay_006289_20	euxassay_006289_21	euxassay_006289_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19812_wholemount_strong.wlz

19812_wholemount_moderate.wlz

19812_wholemount_weak.wlz

19812_wholemount_possible.wlz

19812_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19812_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

strong

regional

strong expression: see section 07 08 09 14 15

submandibular gland primordium

weak

regional

weak expression: see section 06 07 08 09 16 17 18 19

brain

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

facial vii ganglion

strong

regional

strong expression: see section 04

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 moderate expression: see section 16

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 moderate expression: see section 09 16 17 18 19 20

vagus x ganglion

strong

regional

strong expression: see section 07 moderate expression: see section 15

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 16

spinal cord

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 13 14 15

renal cortex

weak

regional

weak expression: see section 06 07 08 09 10 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8813
Entity Detected:	Arnt2, aryl hydrocarbon receptor nuclear translocator 2 ( MGI:107188)
Sequence:	sense strand is shown >T8813 GGCGGCGCTGGCCCGCGCCGGGTCCCCGTCCCCGCCCCCGCCCCCGCCCGGCGCTCCGGTCCCTTCCCGG CAAGATGGCAACCCCGGCCGCCGTCAACCCTCCGGAGATGGCGTCAGACATACCAGGATCTGTGGCCTTG CCTGTTGCCCCCATGGCAGCCACCGGACAGGTGAGAATGGCAGGGGCCATGCCTGCCCGAGGAGGAAAGC GTCGATCCGGAATGGACTTCGATGACGAAGATGGTGAAGGTCCCAGTAAATTCTCAAGAGAGAACCACAG TGAGATTGAGCGGCGCAGGCGGAACAAGATGACTCAATATATTACGGAACTCTCCGACATGGTTCCCACC TGCAGTGCACTGGCTAGGAAGCCAGACAAGCTGACCATCCTGCGCATGGCGGTCTCGCACATGAAGTCCA TGAGGGGCACCGGCAACAAATCGACTGACGGCGCCTACAAGCCTTCCTTCCTCACTGAGCAGGAACTGAA GCATCTCATCCTGGAAGCCGCTGATGGATTTCTGTTTGTGGTGGCAGCTGAGACAGGGAGAGTCATCTAC GTGTCTGATTCGGTCACTCCTGTCCTGAACCAGCCACAGTCAGAGTGGTTTGGGAGCACGCTTTATGAGC AGGTGCACCCTGATGACGTGGAGAAACTGAGGGAACAGCTGTGCACTTCGGAAAACTCCATGACAGGCCG CATCCTGGACCTGAAGACTGGGACAGTGAAGAAGGAGGGACAGCAGTCATCCATGCGCATGTGTATGGGC TCTCGGCGCTCCTTCATCTGTAGGATGAGGTGTGGAAACGCTCCCTTGGACCACCTGCCTTTGAACAGAA TAACCACCATGAGGAAAAGGTTCAGGAATGGCCTTGGCCCTGTGAAAGAAGGAGAAGCCCAGTATGCTGT GGTCCACTGCACAGGTTACATCAAGGCTTGGCCACCAGCAGGAATGACCATACCCGAAGAAGACGCTGAT GTCGGACAAGGCAGTAAATATTGCCTCGTGGCAATTGGGAGGCTCCAGGTGACCAGCTCTCCTGTGTGCA TGGACATGAGCGGCATGTCAGTGCCCACAGAGTTCCTGTCACGGCACAACTCTGATGGGATTATCACGTT TGTGGACCCCAGATGCATCAGTGTGATTGGCTACCAGCCCCAGGACCTTCTGGGAAAGGATATTTTGGAA TTTTGCCACCCTGAGGATCAGAGCCACCTACGGGAGAGCTTCCAACAGGTGGTTAAGCTGAAGGGCCAAG TGCTGTCGGTCATGTATCGGTTCCGCACCAAGAACCGGGAGTGGCTGTTGATCCGTACCAGCAGCTTCAC CTTCCAGAACCCCTACTCTGATGAGATCGAGTACGTCACCTGCACCAACACCAATGTCAAGCAACTTCAG CAACAGCAGGCAGAACTGGAGGTACATCAGCGAGATGGGCTGTCGTCATATGACTTATCTCAGGTCCCAG TACCCAACCTACCCGCTGGTGTTCACGAGGCCGGGAAGTCTGTGGAAAAGGCAGATGCAATCTTCTCCCA AGAGAGAGACCCTCGTTTTGCTGAGATGTTTGCAGGCATCAGTGCATCTGAGAAGAAGATGATGAGCTCA GCCTCAGCATCAGGCAGCCAGCAGATCTACTCCCAAGGAAGTCCATTCCCTGCCGGGCACTCGGGCAAGG CCTTCAGCTCTTCCGTGGTCCATGTGCCTGGAGTGAATGACATTCAGTCCTCCTCCTCAACGGGACAGAA CATATCCCAGATCTCTCGGCAGCTGAACCAGGGCCAGGTGGCATGGACAGGCAGCCGTCCACCGTTCCCA GGGCAGCCCAGCAAGACGCAGTCATCTGCCTTCGGAATTGGATCAAGCCACCCTTACCCGGCTGACCCTT CATCCTACAGTCCTCTCTCCAGCCCAGCTGCCTCCTCACCAAGTGGAAACGCATACCCCAGTCTTGCCAA CAGGACTCCAGGGTTTGCTGAGAGTGGACAGAGTGGCGGGCAGTTCCAGGGCCGGCCCTCGGAGGTCTGG TCCCAGTGGCAGAGCCAGCATCACGGACAGCAGAGCGGTGAGCAGCACTCGCATCAGCAGCCTGGCCAGA CTGAAGTGTTCCAGGACATGCTACCCATGCCGGGCGACCCGACGCAGGGGACTGGCAACTATAACATCGA GGACTTTGCTGACCTGGGCATGTTCCCTCCATTTTCTGAGTAGCTTCAGGCAAAGCCAGGCTTCTACTGC CCAGACGCTATTATCATGCCATAGATGCCCATGTTCAAAGAGGGTCCCCCTCACCCAGCTTGCCCTGCTG TAGAACCCCCAGAAATCTCCCTTATTCCCCATCCTCCCTGGACATGGCATCACCTGGCTCTAACCCACAG CCCTGGCTTCTTGGGTTTGGGATCTTTGTATTTATTGTACTTTATCTGTGTGCTCGGAAGGGGGCCGCCA GGGTCTTAAATCCCATTGTGAATAATCTATTAATGGACAATTTGCACCCATCAAGTGTTTGCTTGATCCA CAATGGCTGGCAGTACCAGCAGGTCTGCCTGGTGCAGAAGCTGGGTTTCTTTCTGCTTTTTCACGGCTGC TGCCTCGGAGGCTTGGTGTCAGAGTGAAAGGCAGTGACCTCTGCATTCTGTGCTGGAGCCAGGAGCACAG GATGGGAGTCTCCCACACTAGCTGTCCTTGACGGCCTGGGGCCAGGTGTGCTTTAAACCTGAGAATCAAC GTTGTGGCTTGGTAGATGTACTGTGGCGTGTTGGTGTGTGTGGCCAGTGAGAGTGAGAAGCCGTCCCCAT TCAGCTGCATTTCAAAGGAAAGCGTGACTGGGGCCTGGGAGCTGGGCTGACTCCCTGGAAGCCAGGGCTG GGGAGCTGCCCACTCTGTAGTCAGTTCTTTGGTCCCCTGCCCTTTTACAGACATACTTTACTTCTTAAAG TCTCTAGTCCTTCTGTCCTATGCGAGCACTCACGCTTCTCTTACTAAGCCTGAGGGACAGTGTCCTGATT CCATAAAGGTCACTGCATCCTCCCTGCTCTGCATCTGAGCAGAGCTGTTTTCTGCCCAGCGCCTTCAGAA AGTCAGACCCAGTCCTCCTCTGTACAGGGTTAGGATACCTCGACTCTAAGTGTCCCTGACACCTTCTGCT CAGTAACTTGGAAAGAAGATGGGGCCACACTCTGGGTGGAAAAGGGTGCCTTGGGCCACCTGAACCTCTG TCCTCATCAGGCAGGAGAGGCAAGGGTACCTCAGGGCAGACACAGCCAGGCGCCTGTGAGGCCAGAGACT CTGTTCTGCCCAAGCTTCTACAAAGACCTGAATTCCTCAGTGGTCTTAAGGTAGAACTATGAACTAACTT CTAGAACAATGGTTCTCAGCCTGTGGGCTGTGTTGGGGATGCCAGACAACCATTTCACAGGGGCCGCATA TCAGATACCCTGCATATTAGGTAGTTACAAACGATACATACATAACAGTAGAAAATCACACTTATAAAAT AGCAATGAAAATGATCTTACGGTTAGGGTCACCACTACATGAAGAACTATATTAAAGGGTCACAGAGTTA GGAAGGTTGAGAACCACTGTTCTAGAAGTGTCTTGCTGGCTTATCTCTGACATCAGAGTCCCTGTGTCAG TCAGATTTAAAGAGCTTCAGATTCCACCCATTCCACCCTGCCCCTCCCTCACTCCATGTGCTGCTCTGAG TTCTCTCTGGCTTTTGGGAAGCTGGGAGTACAAGAGGCTCCAGGGTGCGCAGGGGCGGAATGAGCCTGGA GATTAAGAGCCTGGGGGCACCACTCCATTCAGAAGCTAAGCTCTGCCAGTGTTTGCACAGGACTCACTTG CAAAGTTTGCTTGGCTCAGCAGACTGCCCTTGGCAGCAACCCACCCTAGGGGATCCCGTTCCTGACAAAC TCCAGCCCCAGAGACCTTAGGCAGCTTTGAATCCTTCCATGGAGAGTAGTGACACAGTCAGAGCTTCATC CCAGACTCAGAACCCCCAAACAGTCTCTCCTGGAGCTGGCCCCAGGTGGTGAGCATAAGTTACAAAAGGG AAAACAAGGCCCAAAGCTGGCTTCAGGAGCAAAGAATGCTTTGTAGTGTAGAACAGCCCTTGACCTTTAG CTTGTGAATTAAGAGGTGTGGAAGCTATGGGTTTGGTTTTATTGCCTTGGGTGGGAAGTGGGGGCTTTTC TTGGGTGTGACAGCCCTGGGCTCAGGGCCCTAAGGTCTGCAGAAGAAATAGGACAGGATTCTTGCTTGCT ATCATTTCATGCTTTCTCTCTGTTAAGAAGTTGAGGGTAAAGCCAACTCAGTCCTTGGTCCTGGGAGCAG AGGTGGAGGATTGTTCGGCAACCCAGACAATAGCAAGCACTGCTTTCAATGGTTCCTGCGTCTCCCCAGT CTTATCTAGGGAGTTCCATGGGCTAGAAGGTGGGGATTTAGGCGGGCCTGAGTTCTGGATCTTTTCCATA CCTCTTCGGCTTCTATGTTGTGATTCTCCAAAACACATGTGGAGGAGGTGGTGGGGGGAGAAGTCAGAAA TGAAGAATCCCACCCCACAAGGACTTCTCTCACCCAAGTATTTTTTGAAACACACAGGAGTCAGAATCAA TGCCATTATCCTGCAAAAGATGTCCCTTCATATGTCCCCTGCAGAGGAAGCCAATATACTGGTCCTCCAG TTAATATCCTTCCAGCTACCTGTCCTGGTGATGGTCTTAGCAGGCTAGGGATGGATAGGGTATGCTGGCT TCCTGCAGTTCTATACCTCATCACTTCATTCGCTGCTGAAGTCCAATTGGCTCCTCGTGGGCAGAGCAAG CAGGAAAGGGACATGCAAGGACCACCTGAGTCATTGGTAAGGACACAGGAGCTCTAGAAGAATGGGCCAT ACATAGACTGCCCGGCCAGCCAAGGATGGTTCTAGAACCCTGCCTCCCTGTCACTCCTGTTTGTTTCACT GTGGTTTCAGGGAGCGGGTGAGCCAGCGTGCGCATCCACATTCATCAAGCTCAGCTCAGCGTGTTCTTGC ATCATAAAGTGACTATTGTGGGTAGTAGTATTTGGATTGGTGACATAACCTGCTGAGGAACTCCCACTCC CCTGTCATTAGGATAGTGAGGCCCCAGGTTAGCCCACTGGCAACCAAGCACACTCCCTTCAGCTCTGAGC TAGAGATCTGGCAGGTCCAGGAAGTGAGTTCTGCAATCTCCTTCTGCAACACAGTCCAGCCGATGAATAG TAGCCACTCCCAATGTGAGCTCAGCCTGTCTCACAAGCAAAAACAAGCCGTTTGCTAATCCCTAATAGCA AGGCAAAATTAAAGCCCTCTTTGTATGTGGCCATGGATTGCTATGTCCTAACTTGCCCATCCATCCTTCC CTCCCATCCCCTCCTGAGCTGATGCTCACCTAAAGTGCCACCACTGGAGATATTGAAGTAAGAAGGGTCA TGAGCTCAGGAGTTCAAGACCTACCTGGGCAACATAATGAAACTAATCTTAAAGACAAATATATAGCTGA GTAACTTGGCCACCTTTCATGTGGTGAGAAGTCACCAGCTCCCTGGCTGCCAGCTTCCTCCATTGCCTAG AATCTGGGACAGCTTTGAAGACATAGCTCCATGTGTGACATGCCATGTCTTGTGCACTAAGCTGGGCTAG TTCAGGTAGCCTGCAAATCCTTGTGGCTACTGGCTATAACAAAATGGAGCTTTTCACAGGTCATCCAGGG CCCAGCTGTTTGCTTTGTCTAACCGTCTCTTTCCTTAAAGAAAACTTCACAAGCAGGCTTCCAAGAACAG CAAGCACTTACTCGTCTCTCTCTTAGCCTTTCTTATGGATGTGAACTGTGCTGTGGGTAAATCATTTGTA TTTCTTGAATGTTCTGTATGACTAACAGGTATTAAGTCACTGGGTATATGTATAACTAATGTAACTGCCT TTTAAATTTTCATTACAATAAAAAATGACTTTGCTCTGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6409636 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6409636 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19813 same embryo
	EMAGE:19814 same embryo
	EurExpress:euxassay_006289 same experiment
	MGI:4823251 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source