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EMAGE:19839

Adora2b adenosine A2b receptor ( MGI:99403)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19839 EMAGE:19839 EMAGE:19839 EMAGE:19839 EMAGE:19839
"Pseudo-wholemount" of euxassay_006394. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006394_01 euxassay_006394_02 euxassay_006394_03 euxassay_006394_04
EMAGE:19839 EMAGE:19839 EMAGE:19839 EMAGE:19839 EMAGE:19839
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euxassay_006394_15 euxassay_006394_16 euxassay_006394_17 euxassay_006394_18 euxassay_006394_19
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euxassay_006394_20 euxassay_006394_21 euxassay_006394_22 euxassay_006394_23 euxassay_006394_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19839Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19839_wholemount_strong.wlz
19839_wholemount_moderate.wlz
19839_wholemount_weak.wlz
19839_wholemount_possible.wlz
19839_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19839_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35097
Entity Detected:Adora2b, adenosine A2b receptor ( MGI:99403)
Sequence:sense strand is shown

>T35097
AGTGCATGCCATCAACTGTATCACCCTCTTCCATCCAGCCCTGGCCAAGGACAAGCCCAAGTGGGTGATG
AATGTGGCCATCCTCCTGTCACATGCCAATTCAGTTGTCAACCCCATTGTCTATGCCTACAGGAACCGAG
ACTTCCGCTACAGTTTCCACAAGATCATCTCCAGATATGTTCTCTGCCAGGCCGAGACCAAGGGTGGGAG
TGGGCAGGCTGGGGCGCAGTCTACTCTCAGTCTGGGCTTATGACCTAGGCTCTGGCCTTTTGGAGAAGAA
AGCTTAAAATAAACAATGGGACTAGACACAACTGGCCGATCCTCACTGTGAAAGACAGCTGCACCTCCCA
AGCAAATGGCCCGTCTCCCCTGAATGCTTGCCTGGAGTTACACAAGTCTGGCTGACATGCAAATTGGAGG
CTCCGTGGCTAACAAATACATTTATGAATCTATTCAGCTGCTCTTACTGTGTGGATGGTGGTGGCAGCTT
GAATTGATTCCGGAAAACTGTTTAAGAATCTGCCTCATTCGTGGTAGAAAATGACTGAAACTTACCTTAC
TGTGAAACACTGTGAACTCTTATAATGTAAGTATTTTTCACTTACAGGAATAGAAAAATAAAAGTTGGCT
CTACTAATGAATACTTGTTCCTGGGGCAGCAACTCAGAAAACTGAAGTATAAATCTTCAGTCAAGATACA
GTAGTATTAATTTAGGGAATGATTCTATCCTAAGCTTTGAAGTAGAATTATAATAACAAAACATTTAAGT
TCAATATTTTCCCAGTACACAAAACACTAGAGGTAGAAACTGGAGAGACGAAGTCCTTCCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 71437. Forward Primer - name:071437_F_cDNA_Adora2b, sequence:AGTGCATGCCATCAACTGTATC; Reverse Primer - name:071437_N_SP6_cDNA_Adora2b, sequence:GAGGAAGGACTTCGTCTCTCCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19837 same embryo
 EMAGE:19840 same embryo
 EMAGE:19835 same embryo
 EMAGE:19838 same embryo
 EMAGE:19836 same embryo
 EurExpress:euxassay_006394 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS