EMAGE mouse gene expression spatial database

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EMAGE:19920

Nwd1 NACHT and WD repeat domain containing 1 ( MGI:2442268)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012570. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012570_01	euxassay_012570_02	euxassay_012570_03	euxassay_012570_04

euxassay_012570_05	euxassay_012570_06	euxassay_012570_07	euxassay_012570_08	euxassay_012570_09

euxassay_012570_10	euxassay_012570_11	euxassay_012570_12	euxassay_012570_13	euxassay_012570_14

euxassay_012570_15	euxassay_012570_16	euxassay_012570_17	euxassay_012570_18	euxassay_012570_19

euxassay_012570_20	euxassay_012570_21	euxassay_012570_22	euxassay_012570_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19920_wholemount_strong.wlz

19920_wholemount_moderate.wlz

19920_wholemount_weak.wlz

19920_wholemount_possible.wlz

19920_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19920_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon floor plate

moderate

regional

moderate expression: see section 13

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 moderate expression: see section 13 14

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 10 11 15 16 17

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 16 17 18 19 20 21 22 23

medulla oblongata floor plate

moderate

regional

moderate expression: see section 12 13

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 08 09 10 11 14 15 16

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 14 15 16 17 18

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 11 14

metencephalon floor plate

moderate

regional

moderate expression: see section 12 13

pons mantle layer

strong

regional

strong expression: see section 06

pons ventricular layer

strong

regional

strong expression: see section 07 08 09 11 14 16 17 18 19

midbrain floor plate

moderate

regional

moderate expression: see section 13

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 14 15 16 17

spinal cord ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35806
Entity Detected:	Nwd1, NACHT and WD repeat domain containing 1 ( MGI:2442268)
Sequence:	sense strand is shown >T35806 CCACTCTTACTGGGTGTCACAAAGGCATCACCGCCATAGCATGGAGCCTGGAGGAGAAACTCCTGGTGGT GGGCACCCAGGATGGTGCCATGGTCGTGTGGGACGTGGAGGAACAACAGGTGGTACACGTTCTAATGGGA CACACAGCTGAAGTGAAGTGTGTGCGAGTGTTTGCCCAAGGGACTCTTGCCATCTCTGCATCAAAGGACC ATACCCTGCGTCTGTGGAGTCTGCTTTCCGGCCAGGAGAAAGTCACCATTTTGGATGGAGGGTCACAAAA TCCCACAGAGCCCCAGAGTTGGGACCTTCATGTGGATGAGAGAAACAATGTTGTATACTCGACATCTGGT GCAAGGATCAACATGTGGAATCTGGAAACTTCGAAGCTGGTTTTCTGTATCACAGGAGATGTGTCTGATC CCTGGGTCTGTGTGGCTTTGTTGGCTGCCCAGGGTTTGCTGCTTGCCCTGTCCAAGGGTGGCCAGGTCAG TCTGTGGAGCTCAGCCATGGGAAAACTGCAGGAGAAACACCAGTTATCCAGCATCAAAGAAGAGACACCC ACCTGTGCCGTCTCCATCCAGTCCCGGGCAAGGCTGGTCGCTGGCTTCAGCAGTGGCTCCATTGCCCTGG TCTCCGCTGGAGAAGACAGGCTGCTGGAGAAGCTCCCAGAAGCTGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 69082. Forward Primer - name:069082_F_cDNA_A230063L24Rik, sequence:CCACTCTTACTGGGTGTCACAA; Reverse Primer - name:069082_N_SP6_cDNA_A230063L24Rik, sequence:CACAGCTTCTGGGAGCTTCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19922 same embryo
	EMAGE:19923 same embryo
	EMAGE:19921 same embryo
	EurExpress:euxassay_012570 same experiment
	MGI:4826838 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source