EMAGE mouse gene expression spatial database

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EMAGE:19923

Mpeg1 macrophage expressed gene 1 ( MGI:1333743)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009358. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009358_01	euxassay_009358_02	euxassay_009358_03	euxassay_009358_05

euxassay_009358_06	euxassay_009358_07	euxassay_009358_08	euxassay_009358_09	euxassay_009358_10

euxassay_009358_11	euxassay_009358_12	euxassay_009358_13	euxassay_009358_14	euxassay_009358_15

euxassay_009358_16	euxassay_009358_17	euxassay_009358_18	euxassay_009358_19	euxassay_009358_20

euxassay_009358_21	euxassay_009358_22	euxassay_009358_23	euxassay_009358_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19923_wholemount_strong.wlz

19923_wholemount_moderate.wlz

19923_wholemount_weak.wlz

19923_wholemount_possible.wlz

19923_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19923_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36642
Entity Detected:	Mpeg1, macrophage expressed gene 1 ( MGI:1333743)
Sequence:	sense strand is shown >T36642 CTGTCAGGCTCCCACCTTATACCAAACCACCTCTTATGAGCCAGGTTGCCACCAACACTGTCATAGTGAC CAATAGTGAGACTGCCAGATCCTGGATTAAGGATCCTCAGACCAACCAGTGGAAGCTGGGAGAACCTCTG GAGCTTCGTAGGGCCATGACAGTCATCCATGGGGACAGTAATGGAATGTCAGGAGGGGAAGCTGCTGGAA TCACTTTGGGAGTCACCATAGCACTAGGAGTTGTCATTACCTTGGCCATCTATGGTACTCGGAAGTACAA GAAGAAGGAATACCAGGAAATTGAGGAGCAGGAGAGTTTGGTTGGAAGCTTAGCAACAGATGCAACAGTC CTTAATGGAGAAGAGGATCCAAGTCCAGCTTAATTGTCTCCAAAGGAAACAGTTTCCAGCCACAGCTTCA AGCACATCTTTTGCTTTGTTTTCTCTACTTCTGCCTTCCTAAGTGACTGAAGTGACAGTCGCCATAGGAA GAAAGCAGCTATTACAACCCTGGCAGTATTTTCTTAGGCCTCTGCACCAGGAAATTAAAGGAGCCATTTG AAGTGAGGTGTGGGGAGGGGTATGATTCATTTGGAAAAAGCTGGACTGGGACTAAGCCTCGGGGTGTATG CGTCCATTCTCATAGTTGTCTGTACCTGAATGCATGTGTGTGGAACGAGGGGTGGGGGAGAAATTGGATT TGGTGCATTTAGTCTGAATTTGAAAGGCTTTCCAGTTTGTAAGCTGAAGAGGTCTGTGCTTTAAGTACAT TACTTCTTTTCTTGTGTTTTCAAAGAGGCCCTCAGGGTTTCAGTAAACAGTGCCCTGAAATGCCACAGGA GGCCTACATGCCCCCTACTCTTTAGCTTTAAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 86031. Forward Primer - name:086031_F_cDNA_Mpeg1, sequence:CTGTCAGGCTCCCACCTTATAC; Reverse Primer - name:086031_N_SP6_cDNA_Mpeg1, sequence:CTTAAAGCTAAAGAGTAGGGGGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19922 same embryo
	EMAGE:19921 same embryo
	EMAGE:19920 same embryo
	EurExpress:euxassay_009358 same experiment
	MGI:4826427 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source