Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19944

Rc3h2 ring finger and CCCH-type zinc finger domains 2 ( MGI:2442789)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19944 EMAGE:19944 EMAGE:19944 EMAGE:19944 EMAGE:19944
"Pseudo-wholemount" of euxassay_009447. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009447_01 euxassay_009447_02 euxassay_009447_03 euxassay_009447_04
EMAGE:19944 EMAGE:19944 EMAGE:19944 EMAGE:19944 EMAGE:19944
euxassay_009447_05 euxassay_009447_06 euxassay_009447_07 euxassay_009447_08 euxassay_009447_09
EMAGE:19944 EMAGE:19944 EMAGE:19944 EMAGE:19944 EMAGE:19944
euxassay_009447_10 euxassay_009447_11 euxassay_009447_12 euxassay_009447_13 euxassay_009447_14
EMAGE:19944 EMAGE:19944 EMAGE:19944 EMAGE:19944 EMAGE:19944
euxassay_009447_15 euxassay_009447_16 euxassay_009447_17 euxassay_009447_18 euxassay_009447_19
EMAGE:19944 EMAGE:19944 EMAGE:19944
euxassay_009447_20 euxassay_009447_21 euxassay_009447_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19944Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19944_wholemount_strong.wlz
19944_wholemount_moderate.wlz
19944_wholemount_weak.wlz
19944_wholemount_possible.wlz
19944_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19944_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36637
Entity Detected:Rc3h2, ring finger and CCCH-type zinc finger domains 2 ( MGI:2442789)
Sequence:sense strand is shown

>T36637
AAGTCCCACAATACCCACAAACAGGATACTACCCACCACCTCCAACGGTACCAGCTGGTGTGACTCCCTG
TGTTCCTCGCTTTGTGAGGTCCAGTAATGTTCCAGAGTCCTCCCTCCCACCTGCTTCCATGCCATATGCT
GATCATTACAGTACATTCTCCCCTCGAGATCGAATGAATTCTTCTCCTTACCAACCTCCTCCTCCGCAGC
AGTATGGACCAGTTCCTCCAGTACCCTCTGGAATGTATGCTCCGGTGTATGACAGCAGGCGCATCTGGCG
CCCAGCTATGTACCAACGAGATGACATTATTAGAAGCAATTCTTTACCTCCAATGGATGTGATGCACTCA
TCTGTCTATCAGACATCTTTACGGGAAAGATACAACTCATTAGATGGATATTATTCAGTGGCTTGTCAGC
CACCGAATGACCCAAGGACAACTGTGCCTTTACCAAGGGAACCTTGTGGTCATTTGAAGACCAGTTGTGA
GGAACAGTTAAGAAGAAAGCCAGATCAGTGGACACAGTATCATACCCAGAAAACACCTGTCTCTTCAACT
CTTCCTGTGGCCACACAGTCACCAACACCACCTTCTCCTCTATTCAGTGTAGACTTCCG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 76462. Forward Primer - name:076462_F_cDNA_Mnab, sequence:AAGTCCCACAATACCCACAAAC; Reverse Primer - name:076462_N_SP6_cDNA_Mnab, sequence:CGGAAGTCTACACTGAATAGAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19945 same embryo
 EMAGE:19943 same embryo
 EMAGE:19942 same embryo
 EurExpress:euxassay_009447 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS