EMAGE mouse gene expression spatial database

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EMAGE:19956

Pard3b par-3 partitioning defective 3 homolog B (C. elegans) ( MGI:1919301)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009412. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009412_01	euxassay_009412_02	euxassay_009412_03	euxassay_009412_04

euxassay_009412_05	euxassay_009412_06	euxassay_009412_07	euxassay_009412_08	euxassay_009412_09

euxassay_009412_10	euxassay_009412_11	euxassay_009412_12	euxassay_009412_13	euxassay_009412_14

euxassay_009412_15	euxassay_009412_16	euxassay_009412_17	euxassay_009412_18	euxassay_009412_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19956_wholemount_strong.wlz

19956_wholemount_moderate.wlz

19956_wholemount_weak.wlz

19956_wholemount_possible.wlz

19956_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19956_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

weak

regional

weak expression: see section 10 11 12 13 14

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 10 11 12 13

telencephalon ventricular layer

weak

regional

weak expression: see section 01 02 03 04 05 06 08 09 10 11 13 14 15 16 17 18 19

medulla oblongata alar plate ventricular layer

weak

regional

weak expression: see section 07 08 09 10 14 15

medulla oblongata basal plate ventricular layer

weak

regional

weak expression: see section 11 12 13

rest of cerebellum ventricular layer

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

pons ventricular layer

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16

midbrain ventricular layer

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 14 weak expression: see section 10

vomeronasal organ

moderate

regional

moderate expression: see section 11 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36816
Entity Detected:	Pard3b, par-3 partitioning defective 3 homolog B (C. elegans) ( MGI:1919301)
Sequence:	sense strand is shown >T36816 AGGGAGAGACAGTGTCATTGGTCATTGCCCGCCAGGAAGGGAGCTTCCTGCCCCGAGAGCTGAAAGGAGA ACCTGATTGTTATGCTCTCTCCCTGGAGTCAAGCGAGCAGCTCACCTTGGAGATCCCCCTGAATGATTCT GGCTCCGCTGGCCTGGGGGTTAGCCTGAAAGGGAATAAGTCTCGAGAAACTGGAACTGACTTGGGGATTT TTATCAAATCCATCATCCATGGAGGTGCTGCTTTCAAGGATGGCCGTCTCCGAATGAATGACCAACTGAT TGCTGTTAACGGGGAAACTCTTCTGGGAAAGTCCAACCACGAAGCCATGGAAACACTCAGGCGATCCATG TCCATGGAGGGAAACATCCGGGGCATGATCCAGTTGGTGATTCTGAGGAGACCTGAGAGACCACTGGAGG AGCTTTCAGAATGTGGAGCACTTTCCAGACCAGGCTTTGAGAACTGTCAAGAAGCTCTGAGCACCTCCAG GCGAAATGATAGTAGTATATTATATCCGTTTGGCACCTATAGTCCACAAGATAAAAGGAAAGACCTATTG CTTCCCAGTGATGGGTGGGCTGAGAATGAAGTACCACCGTCCCCGCCACCACATCCTGCTCTGGAATGGG GCCTTGAAGATTTCAGTCACAGCTCTGGAGTGGATTCCACAGGATATTTTCCAGATCAACATGTCAACTT CAGAACGGTGACACCAGTCAGGCAGCCCGAATTGATTAACCTTAAAGCCTCCAAGAGCATGGACCTTGTG CCAGATGAAGGCAAAGTTCAGTCGCTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 75657. Forward Primer - name:075657_F_cDNA_2010002N16Rik, sequence:AGGGAGAGACAGTGTCATTGGT; Reverse Primer - name:075657_N_SP6_cDNA_2010002N16Rik, sequence:CAGCGACTGAACTTTGCCTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19957 same embryo
	EMAGE:19954 same embryo
	EMAGE:19953 same embryo
	EMAGE:19952 same embryo
	EMAGE:19955 same embryo
	EurExpress:euxassay_009412 same experiment
	MGI:4827050 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source