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EMAGE:19992

Sgca sarcoglycan, alpha (dystrophin-associated glycoprotein) ( MGI:894698)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19992 EMAGE:19992 EMAGE:19992 EMAGE:19992 EMAGE:19992
"Pseudo-wholemount" of euxassay_009460. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009460_01 euxassay_009460_02 euxassay_009460_03 euxassay_009460_04
EMAGE:19992 EMAGE:19992 EMAGE:19992 EMAGE:19992 EMAGE:19992
euxassay_009460_05 euxassay_009460_06 euxassay_009460_07 euxassay_009460_08 euxassay_009460_09
EMAGE:19992 EMAGE:19992 EMAGE:19992 EMAGE:19992 EMAGE:19992
euxassay_009460_10 euxassay_009460_11 euxassay_009460_12 euxassay_009460_13 euxassay_009460_14
EMAGE:19992 EMAGE:19992 EMAGE:19992 EMAGE:19992 EMAGE:19992
euxassay_009460_15 euxassay_009460_16 euxassay_009460_17 euxassay_009460_18 euxassay_009460_19
EMAGE:19992 EMAGE:19992 EMAGE:19992
euxassay_009460_20 euxassay_009460_21 euxassay_009460_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19992Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19992_wholemount_strong.wlz
19992_wholemount_moderate.wlz
19992_wholemount_weak.wlz
19992_wholemount_possible.wlz
19992_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19992_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37011
Entity Detected:Sgca, sarcoglycan, alpha (dystrophin-associated glycoprotein) ( MGI:894698)
Sequence:sense strand is shown

>T37011
GTACCAAGTCATCGAGGTCACAGCCTACAATCGAGACAGTTTTGACACCACTAGACAGAGGCTGCTGCTG
CTGATTGGGGACCCCGAAGGTCCCCGGTTGCCATACCAAGCTGAGTTCCTGGTGCGCAGCCATGATGTGG
AGGAGGTGCTGCCCACCACACCTGCCAACCGCTTCCTCACCGCCTTGGGGGGACTGTGGGAGCCAGGAGA
GCTTCAGCTGCTCAACATCACTTCCGCCTTGGACCGGGGAGGCCGAGTCCCTCTTCCTATTGAGGGACGG
AAGGAAGGGGTATACATTAAGGTAGGCTCTGCCACACCCTTCTCCACCTGCCTGAAGATGGTGGCGTCGC
CCGACAGCTATGCCCGTTGTGCCCAGGGACAGCCTCCACTACTGTCCTGCTACGACACTTTGGCACCCCA
CTTCCGCGTTGACTGGTGCAATGTGTCTCTGGTAGACAAGTCAGTACCCGAGCCCCTGGATGAGGTACCT
ACTCCAGGCGATGGGATCTTGGAGCACGACCCGTTCTTCTGCCCACCCACTGAAGCCACAGACCGAGACT
TCCTGACAGATGCCTTGGTGACCCTCTTGGTGCCTTTGTTGGTGGCTCTGCTGCTTACTCTGTTGCTGGC
TTACATCATGTGCTTTCGGCGTGAAGGACGGCTGAAGAGAGACATGGCCACCTCTGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89661. Forward Primer - name:089661_F_cDNA_Sgca, sequence:GTACCAAGTCATCGAGGTCACA; Reverse Primer - name:089661_N_SP6_cDNA_Sgca, sequence:TCAGAGGTGGCCATGTCTCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19989 same embryo
 EMAGE:19991 same embryo
 EMAGE:19988 same embryo
 EMAGE:19990 same embryo
 EurExpress:euxassay_009460 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS