EMAGE mouse gene expression spatial database

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EMAGE:20050

Card6 caspase recruitment domain family, member 6 ( MGI:3032959)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012170. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012170_01	euxassay_012170_02	euxassay_012170_03	euxassay_012170_04

euxassay_012170_05	euxassay_012170_06	euxassay_012170_07	euxassay_012170_08	euxassay_012170_09

euxassay_012170_10	euxassay_012170_11	euxassay_012170_12	euxassay_012170_13	euxassay_012170_14

euxassay_012170_15	euxassay_012170_16	euxassay_012170_17	euxassay_012170_18	euxassay_012170_19

euxassay_012170_20	euxassay_012170_21	euxassay_012170_22	euxassay_012170_23	euxassay_012170_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20050_wholemount_strong.wlz

20050_wholemount_moderate.wlz

20050_wholemount_weak.wlz

20050_wholemount_possible.wlz

20050_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20050_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

spotted

strong expression: see section 23 24

upper leg muscle

strong

spotted

strong expression: see section 04 05 06 07 22 23 24

vertebral axis musculature

strong

spotted

strong expression: see section 04 08 16 23 24 moderate expression: see section 03 05

heart ventricle

strong

spotted

strong expression: see section 06 07 08 09 10 11 moderate expression: see section 12 13 14 15 16

lung

strong

spotted

strong expression: see section 04 05 06 07 08 12 15 16 20 moderate expression: see section 14 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37619
Entity Detected:	Card6, caspase recruitment domain family, member 6 ( MGI:3032959)
Sequence:	sense strand is shown >T37619 AGTCTATAAGGACACTGCCCCACATTAAATATCCTCACCCTCAGCCCTGTCAGCCAGCAGGAGCCAGTCA AGAACGGATAATGCCAGTCTCTCATCAAGGAGCCCAACAAACAACACAGGGAAGGCCTGCGGATTTTGCT TTCAAACCAGGGTCTCAATCTACATCTGGGAGTAAACTTTCATCTACCTCCCAGTCCAGCGCCCATCAAC CCAAATTCCAGAGCAAACACTTCCAGCCTCAGCCGTTTCAACCCGTGCCTTCTCAGAAAAAACCGTCTCA CTCCCGGCCCTCCCAAGCTAAACCCCCTCATCTGGATCCCTCTCATGCAAACCTTACTCAGGGGCAGCCT TCCCAAGCTACACCCACTCACTCCCAGGCCTCCCAAGCTAAACCCACTCACTCTCAAGCTAACTCTCATC ATCCACATCCCTCCCATGCTAAACCTTCTCATCAGAATCCCTCTCATGCTAACCCTACTCATCCGCAGTC CTCCCATGCTAAGCCCTCTCATCCGCAGTCCTCCCATGCTAAGCCCTCTCATCCACAGTCCTCCCATGCT AAGCCCTCTCATCCACAGTCCTCCCATGCTAAGCCCTCTCATCCACAGTCCTCCCAAGCTAAGCCCTCTC ATCCACAGTCCTCCCAAGCTAAGCCCACTCATCCACAGTCCTCCCAAGCTAACTCCCATCATCCACAGGC CTCCCAAGCTAAGCCCTCTCATCCACAGTCCTCCCATGCTAAGCCCTCTCATCCGCATCCCTCCCATGCT AAGCCCTCCCCATCCCAGTCTACTCAGTGTAAGGCACATAAAGCCCATCAGTCCCAACCTAAGCCTTTTC AA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 84926. Forward Primer - name:084926_F_cDNA_Card6, sequence:AGTCTATAAGGACACTGCCCCA; Reverse Primer - name:084926_N_SP6_cDNA_Card6, sequence:TTGAAAAGGCTTAGGTTGGGAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20049 same embryo
	EMAGE:20052 same embryo
	EMAGE:20051 same embryo
	EMAGE:20053 same embryo
	EMAGE:20054 same embryo
	EurExpress:euxassay_012170 same experiment
	MGI:4823637 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source