EMAGE mouse gene expression spatial database

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EMAGE:20111

Fam84b family with sequence similarity 84, member B ( MGI:3026924)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012212. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012212_01	euxassay_012212_02	euxassay_012212_03	euxassay_012212_04

euxassay_012212_05	euxassay_012212_06	euxassay_012212_07	euxassay_012212_08	euxassay_012212_09

euxassay_012212_10	euxassay_012212_11	euxassay_012212_12	euxassay_012212_13	euxassay_012212_14

euxassay_012212_15	euxassay_012212_16	euxassay_012212_17	euxassay_012212_18	euxassay_012212_19

euxassay_012212_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20111_wholemount_strong.wlz

20111_wholemount_moderate.wlz

20111_wholemount_weak.wlz

20111_wholemount_possible.wlz

20111_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20111_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 15 weak expression: see section 05 06 07 08 16 17

vibrissa

weak

regional

weak expression: see section 03 18

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 15 16 17 18 weak expression: see section 01 02 03 19

lateral ventricle choroid plexus

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

stomach

weak

regional

weak expression: see section 06 07

stomach fundus

weak

regional

weak expression: see section 05

lower jaw molar

weak

regional

weak expression: see section 05 17

upper jaw molar

weak

regional

weak expression: see section 04 17

metanephros

weak

regional

weak expression: see section 06 07 09 10 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37655
Entity Detected:	Fam84b, family with sequence similarity 84, member B ( MGI:3026924)
Sequence:	sense strand is shown >T37655 TTGATGACAGTCCCAAACGTAGGTTGGTGGTGGCGGCATTCGGACAGGCTGAAGAGGTAGCAATTGTGTC TGCAACTGAACTTCCGTGCACTTTAGTACAAGGCTTTAAGACTGCGACAGCCTGGTGCCTGAACCGAAGG CAGACCTTTGACTGCCGAGGGCTTATAGGCAGTCTGTCAACTGCTTTCAAAAACTGAGCATACTTAAAGG CCTTTGCTGCTTCCACCAGTTTTTATAACACTGGAACTAGTTCTTAGATTGGAACAAAATCTTACTTGAG TGTCCTGAAGCCACGGTCATTGTTGGGCTTCTTGCTTGGGGAATCTTTGAACTCCCCCTCCCCCCTTTTT TTAATAAGCCTAAATCTTCATAGTACCTCAGTATGAAACGGTTTCGTTAGTTATAAAGGCATCAAATGCT CTTTCTGCTTTTATAATGCTTACCAAGCAAGTCCTTGTTCTTTGAATGTCAAAGATGAGCTACCTACACA CTAGCCTTGGTTTCAGACATTGGAGGAGAACCATTTTCTAGTTAGGAGTATGGATCTGATTTCCCTGTTT AATGAGCTCATAGGTATTTGATTACATAGTCTTGTCCATGGAACAGCAGAGAGAATAAACACAGAGGATA TTATTTAGGGAAACCTATGTCTAACAGATTTGTGAAAGTGGAAATTGATGCCCGCTGTGTAGACTGCAGG AGGGGAGGAATCAGAATGGCTGTCCACCAGCAGCTCTGGAATAGCAAACTCTCCCGGCTCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 73172. Forward Primer - name:073172_F_cDNA_D330050I23Rik, sequence:TTGATGACAGTCCCAAACGTAG; Reverse Primer - name:073172_N_SP6_cDNA_D330050I23Rik, sequence:AGAGCCGGGAGAGTTTGCTAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20108 same embryo
	EMAGE:20110 same embryo
	EMAGE:20107 same embryo
	EMAGE:20109 same embryo
	EMAGE:20106 same embryo
	EurExpress:euxassay_012212 same experiment
	MGI:4824770 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source