EMAGE mouse gene expression spatial database

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EMAGE:20214

Ppp2r1b protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform ( MGI:1920949)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012306. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012306_01	euxassay_012306_02	euxassay_012306_03	euxassay_012306_04

euxassay_012306_05	euxassay_012306_06	euxassay_012306_07	euxassay_012306_08	euxassay_012306_09

euxassay_012306_10	euxassay_012306_11	euxassay_012306_12	euxassay_012306_13	euxassay_012306_14

euxassay_012306_15	euxassay_012306_16	euxassay_012306_17	euxassay_012306_18	euxassay_012306_19

euxassay_012306_20	euxassay_012306_21	euxassay_012306_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20214_wholemount_strong.wlz

20214_wholemount_moderate.wlz

20214_wholemount_weak.wlz

20214_wholemount_possible.wlz

20214_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20214_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 11 12 13 14 15

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 16 17 18

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21

lower jaw incisor

weak

regional

weak expression: see section 12 13

lower jaw molar

moderate

regional

moderate expression: see section 06 07 18

upper jaw incisor

weak

regional

weak expression: see section 12

upper jaw molar

moderate

regional

moderate expression: see section 06 07 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30450
Entity Detected:	Ppp2r1b, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform ( MGI:1920949)
Sequence:	sense strand is shown >T30450 CCAGCCAGAGGAGGAAGAACATGGCGGGCGCAGCAGGGCCAGGGTCCGGTCCGGGGGCAGCGGGTGGAGA TGGAGACGATTCGCTCTACCCGATCGCGGTTTTAATCGACGAGCTCCGCAATGAGGACGTGCAGCTCCGT CTCAACAGTATTAAGAAGTTATCAACTATAGCTCTAGCACTCGGGGTAGAAAGGACTCGAACGGAACTGC TGCCTTTCCTTACAGATACTATTTATGATGAAGATGAGGTGCTGTTAGCTCTCGCCGAGCAGCTGGGAAA TTTCACTGGTTTGGTGGGCGGTCCTGACTTTGCCCACTGTCTGCTGCCTCCTTTGGAAAGTTTGGCTACG GTGGAGGAGACTGTCGTTCGAGACAAGGCTGTGGAGTCGCTGAGGCAGATCTCGCAGGAGCACACTCCTG TTGCCCTTGAAGCTCATTTTGTGCCTCTGGTGAAGCGCCTGGCAAGCGGGGACTGGTTCATGTCGCGCAC GTCTGCATGTGGCTTGTTCAGCGTTTGCTATCCCAGAGCTTCAAATGCTGTCAAGGCAGAGATTCGACAG CACTTCCGTTCTCTCTGCTCGGATGATACACCAATGGAGCGACGTGCTGCTGCTTCCAAATTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:30016074), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58905. Forward Primer - name:058905_F_IRAV106_a10_Ppp2r1b, sequence:CCAGCCAGAGGAGGAAGA; Reverse Primer - name:058905_R_SP6_IRAV106_a10_Ppp2r1b, sequence:CCCAATTTGGAAGCAGCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20213 same embryo
	EMAGE:20216 same embryo
	EMAGE:20215 same embryo
	EMAGE:20217 same embryo
	EurExpress:euxassay_012306 same experiment
	MGI:4827384 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source