EMAGE mouse gene expression spatial database

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EMAGE:20252

Ccdc28b coiled coil domain containing 28B ( MGI:1913514)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012331. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012331_01	euxassay_012331_02	euxassay_012331_03	euxassay_012331_04

euxassay_012331_05	euxassay_012331_06	euxassay_012331_07	euxassay_012331_08	euxassay_012331_09

euxassay_012331_10	euxassay_012331_11	euxassay_012331_12	euxassay_012331_13	euxassay_012331_14

euxassay_012331_15	euxassay_012331_16	euxassay_012331_17	euxassay_012331_18	euxassay_012331_19

euxassay_012331_20	euxassay_012331_21	euxassay_012331_22	euxassay_012331_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20252_wholemount_strong.wlz

20252_wholemount_moderate.wlz

20252_wholemount_weak.wlz

20252_wholemount_possible.wlz

20252_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20252_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 13 17 weak expression: see section 14 15 16

thyroid gland

moderate

regional

moderate expression: see section 17

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 07 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 09 19

spinal cord

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 17 18 weak expression: see section 11

cervical ganglion

moderate

regional

moderate expression: see section 18 19 weak expression: see section 10 11

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 12 14 15 16 17 weak expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31251
Entity Detected:	Ccdc28b, coiled coil domain containing 28B ( MGI:1913514)
Sequence:	sense strand is shown >T31251 CCAGACCCCAAACTTCCAGACCTGAGGTCCAACACACCACCCAATGGAAGACAAAAAGAAGAAACGGAGT CCTAAGCCCTGCCTGACCCAACAAGCACAGGCCCCAGGCACACTGCGCAGAGTCCCTGTGCCCACCAGCC ACAGTGGCTCCTTGGCTCTGGGACTCCCTCATTTGCCATCACCTAAGCAGCGAGCCAAGTTCAAGAGAGC AGGCAAGGAGAAATGCCGACCGGTGCTGGCTGGAGGTGGAGGCGGCTCCGCAGGCACACCCCTGCAACAC TCCTTCCTGACAGAGGTGACAGATGTTTATGAGATGGAGGGAGGACTGCTGAACTTGCTCAATGATTTTC ATTCAGGCCGCCTGCAGGCCTTTGGGAAGGAATGTTCCTTCGAGCAGCTGGAGCATGTTCGGGAGATGCA GGAGAAGTTGGCCAGACTGCACTTCAGCCTGGATGTGTGTGGGGAGGAAGAGGACGAGGAAGAAGAGGAG GATGGGGTCACAGAAGGCTTGCCTGAGGAGCAGAAGAAGACAATGGCTGACCGCAACCTGGACCAGCTGC TTAGCAATCTGGAAGATCTTAGCAATTCCATACAGAAGCTACACTTGGCAGAGAATGCTGAGCCTGAGGA CCAGCCAGCTGCGTAGGTGTCGCCTAAACTCTTCTGCTACTCTCCTCTTCAAACTGTGAATTTTACGGAT TCGGAAGCCCCTTGGTGCTATGGGGTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4220355), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 55725. Forward Primer - name:055725_F_IRAV49_d01_1810010N17Rik, sequence:CCAGACCCCAAACTTCCA; Reverse Primer - name:055725_R_SP6_IRAV49_d01_1810010N17Rik, sequence:CGACCCCATAGCACCAAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20255 same embryo
	EMAGE:20254 same embryo
	EMAGE:20253 same embryo
	EurExpress:euxassay_012331 same experiment
	MGI:4823672 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source