EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:20279

Slc22a8 solute carrier family 22 (organic anion transporter), member 8 ( MGI:1336187)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012390. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012390_01	euxassay_012390_02	euxassay_012390_03	euxassay_012390_04

euxassay_012390_05	euxassay_012390_06	euxassay_012390_07	euxassay_012390_08	euxassay_012390_09

euxassay_012390_10	euxassay_012390_11	euxassay_012390_12	euxassay_012390_13	euxassay_012390_14

euxassay_012390_15	euxassay_012390_16	euxassay_012390_17	euxassay_012390_18	euxassay_012390_19

euxassay_012390_20	euxassay_012390_21	euxassay_012390_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20279_wholemount_strong.wlz

20279_wholemount_moderate.wlz

20279_wholemount_weak.wlz

20279_wholemount_possible.wlz

20279_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20279_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

single cell

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

diencephalon meninges

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

telencephalon meninges

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata alar plate mantle layer

moderate

single cell

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

medulla oblongata basal plate mantle layer

moderate

single cell

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

hindbrain meninges

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

rest of cerebellum mantle layer

moderate

single cell

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

pons mantle layer

moderate

single cell

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain meninges

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain mantle layer

moderate

single cell

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

spinal cord mantle layer

moderate

single cell

moderate expression: see section 10 11 12 13 14 15 16

spinal cord meninges

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31283
Entity Detected:	Slc22a8, solute carrier family 22 (organic anion transporter), member 8 ( MGI:1336187)
Sequence:	sense strand is shown >T31283 GTTGACATTCCCGCCAAGTTCATCACAATCCTCTCCATAAGTTATCTGGGCCGGCGCATCACTCAGGGCT TCCTCCTGATCCTGGCAGGAGTGGCCATCCTGGCCCTCATCTTTGTGTCTTCAGAAATGCAGCTCTTGAG AACAGCACTGGCTGTATTTGGGAAGGGATGCCTGTCTGGCTCCTTCAGCTGCCTCTTCCTCTACACAAGT GAGCTCTACCCTACAGTCCTCAGGCAAACAGGTATGGGTATCAGTAACATATGGGCTCGAGTGGGAAGTA TGATAGCCCCACTGGTGAAAATCACGGGAGAACTGCAGCCCTTCATCCCTAATGTCATCTTTGGGACCAT GACTCTACTGGGAGGCAGTGCTGCCTTCTTTCTGCTTGAGACCCTCAATCGGCCCTTACCAGAAACTATC GAGGACATACAAGACTGGTACCAGCAAACCAAGAAAACAAAGCAGGAGCCAGAAGCAGAAAAGGCATCCC AGACAATCCCGCTGAAGACTGGTGGACCCTAGCTAAGAACAACAGAATCCTCTTTCCTGCCTTCCAGAGA CTGATCCCAAGCAGGGCCCTTCCAAGGCTATTCGAGCACCTTAGGGGTTGGGTGGAGCCCCAGCTGGCTC CATGCTCTCAGAACAAAGACTTCTGAGAGTTCAGCAAAAGTGTTTTACCTTCACCACCTCCACCGTAGCC CACAACCCAGACCTGGCCTGTTCACAGCCCTAGCCATACTCACTCCTGCACTCATCCTCCCTGCAACCCA GGCCCTGCCATTTTTCTCTACCCTCTTTGTATTGGCCATTTCCTCCATTGTCCCACCTCCATTTCCCTTT GAGATTCCCTGGCAGTTCTAATGGTTTCCTCTTACCTTCCCCAAACTCTCTCCTTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4239544), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 55942. Forward Primer - name:055942_F_IRAV50_c08_Slc22a8, sequence:GTTGACATTCCCGCCAAG; Reverse Primer - name:055942_R_SP6_IRAV50_c08_Slc22a8, sequence:CCAAGGAGAGAGTTTGGGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20280 same embryo
	EMAGE:20278 same embryo
	EMAGE:20276 same embryo
	EMAGE:20277 same embryo
	EurExpress:euxassay_012390 same experiment
	MGI:4828140 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source