EMAGE mouse gene expression spatial database

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EMAGE:20306

Prom2 prominin 2 ( MGI:2138997)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012556. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012556_01	euxassay_012556_02	euxassay_012556_03	euxassay_012556_04

euxassay_012556_05	euxassay_012556_06	euxassay_012556_07	euxassay_012556_08	euxassay_012556_09

euxassay_012556_10	euxassay_012556_11	euxassay_012556_12	euxassay_012556_13	euxassay_012556_14

euxassay_012556_15	euxassay_012556_16	euxassay_012556_17	euxassay_012556_18	euxassay_012556_19

euxassay_012556_20	euxassay_012556_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20306_wholemount_strong.wlz

20306_wholemount_moderate.wlz

20306_wholemount_weak.wlz

20306_wholemount_possible.wlz

20306_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20306_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

anterior naris epithelium

moderate

regional

moderate expression: see section 13 14

external naris epithelium

moderate

regional

moderate expression: see section 14

esophagus epithelium

moderate

regional

moderate expression: see section 12

stomach

moderate

regional

moderate expression: see section 02 03 04 05 06 07

bladder

moderate

regional

moderate expression: see section 11 12

urethra of male

moderate

regional

moderate expression: see section 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31430
Entity Detected:	Prom2, prominin 2 ( MGI:2138997)
Sequence:	sense strand is shown >T31430 CACTTTCCTGGTGGGTGGCAATGTGCAGACGCTGGTGTGCCGGAGCTGGGAGAGTGGAGAGCTGTATGAG TTTGCAGACACTCCGGGGAACTTGCCACCATCCATGAACCTATCCTATCTTCTTGGCCTGAAGAAAAACA TCAGTATCGTCCAGGCCTATCGACAGTGTAAGGCAGGGGCAGTACTCTGGAAGGTTCTACAGCTCAATGA CTCGTATGACCTGGATAAGCACCTGGATATCAAACAGTATACCCACAAGATTCAGCAGGAGCTTCAGAGC TTCCAAGTAGATTTGAAGGAGCTAGACCTGTTGAGCCCCACTGCCCGCCAGGACCTGGAGGCTTTGCAGA GGAGCGGGCTGGAGAAAATCCACTACAGAGGCTTCCTTGTGCAGATCCAGAAGCCTGTGGTGAACACTGA CATGTGGCAGCTGGCGCAGGAGCTGGAAGGACTGGCCCAGGCCCAAAATGACTCTTTACTGAGGCAGCAG CTACAGGAGGAGGCCAGAGAACTTCGAAGCCTCTACCAGGAGAAGGTTGTCCCCCAGGAGAGCCTTGTGA CCAAGCTCAACTTCAGTGTCAAGACCTTGGAGTCCTTGGCCCCAAGTCTGCAGGTGAATACCTCAGACTT TCTTGACAGTGTCACCCGTCTAAAAGGAGAGCTGCCTGTGCAGATCAACCACATCCTGAGGAATGCCACT GAGTGTTTCCTGACCAGGGAAATGGGCTACTTCTCTCAGTATGTGACCTGGGTGAGAGCAGAGGTGACTC AGCGCATCGCCACCTGCCAGCCCTTCTCTACAGCCCTGGACAATGGTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4188940), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 26411. Forward Primer - name:026411_F_IRAV59-62_D23_Prom2, sequence:CACTTTCCTGGTGGGTGG; Reverse Primer - name:026411_R_SP6_IRAV59-62_D23_Prom2, sequence:TGACCATTGTCCAGGGCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20304 same embryo
	EMAGE:20307 same embryo
	EMAGE:20308 same embryo
	EMAGE:20303 same embryo
	EMAGE:20305 same embryo
	EurExpress:euxassay_012556 same experiment
	MGI:4827438 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source