EMAGE mouse gene expression spatial database

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EMAGE:20310

Fgfr1op2 FGFR1 oncogene partner 2 ( MGI:1914779)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012453. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012453_01	euxassay_012453_02	euxassay_012453_03	euxassay_012453_04

euxassay_012453_05	euxassay_012453_06	euxassay_012453_07	euxassay_012453_08	euxassay_012453_09

euxassay_012453_10	euxassay_012453_11	euxassay_012453_12	euxassay_012453_13	euxassay_012453_14

euxassay_012453_15	euxassay_012453_16	euxassay_012453_17	euxassay_012453_18	euxassay_012453_19

euxassay_012453_20	euxassay_012453_21	euxassay_012453_22	euxassay_012453_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20310_wholemount_strong.wlz

20310_wholemount_moderate.wlz

20310_wholemount_weak.wlz

20310_wholemount_possible.wlz

20310_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20310_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31336
Entity Detected:	Fgfr1op2, FGFR1 oncogene partner 2 ( MGI:1914779)
Sequence:	sense strand is shown >T31336 AGTTGGTGCTCACCTGCCCATTTGCTCGGCCCTCATCAAAGGCAGTTCATGTTTAAAGACTATAGGAATA CGTCACTTGCATCACAAACAGAAGGAACATACTTACACCTAATTCCCATAACTCTTAGCTAGCAGTTATT CACGACTTGCTTAAGTGTATGCATCTCAGGAAAGAAAGACAAAGAATATACTTTCTAAGAAAGACAGCCA TATATAGACACATTTAGTAGGTTGAACTACTTTGAAAGATTATGTTTTGGTTCTCTGTTAACAATGAACC TGGTTCTTCCTCCTCTTTTCATCATGTTTAAGGATACAAGACAGTTCACCAATAGACAAGAGTTGACAAT TTAATGTAATGAGATAGGTATGGTCATTTCCAAGTTAATTTGGAATTCCTTTATTGTTATTTAAGAAATT ACTCTTCATACACTTACAAAGTAGATACAGGTTCCAAAAATCAAAGTTCTGAGTCTGAGTCTAAGCCTCA TCTAGTTCTTATAACCTGTGAGGTTTTTCCTACAACCTGTGACTCAGAAACTGGCAGTGAAGTGTGGACA CAAAGGGATTGTGCTTTGCACTTTAAAAAAAAAAAAAGCTTCTTAAGGCCACAGGATACAGAAGTGTGCT TTCATACCAACTGCAACCTAAACTTTTCTGTAGTGATAAGAATAAAAGATTATGAGATATGGCTGAAAAT ACACTATACTTGTATCTGAAGTCCATACTGAAATTAGCACAGACAGAAAGTTAGACTTTTCAATATATCA TTTGTTAAGTACATATTACTTTGCAGTGTAATTTTATGTGTAATTTCATGTATTGGCAAAATTAAAAGGA CTTTTCACTCCAGAACCTTTCATTCTGAAGTTTGAGGTGAGTGGGGTCATAGGTCAGTTAGGAAAGGGCC AGTTCCCCAGATGTTAAACCGTTCTCATGCAGAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4504364), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 20230. Forward Primer - name:020230_F_IRAV51-54_K11_Fgfr1op2, sequence:AGTTGGTGCTCACCTGCC; Reverse Primer - name:020230_R_SP6_IRAV51-54_K11_Fgfr1op2, sequence:GCCTCTGCATGAGAACGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20309 same embryo
	EMAGE:20312 same embryo
	EMAGE:20311 same embryo
	EurExpress:euxassay_012453 same experiment
	MGI:4824847 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source